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Article: Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells

TitleIndividual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells
Authors
KeywordsDeoxynivalenol
Nivalenol
Zearalenone
Fumonisin B1
Cytotoxicity
Intestinal epithelial cells
Issue Date2013
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/foodchemtox
Citation
Food and Chemical Toxicology, 2013, v. 57, p. 276-283 How to Cite?
AbstractFusarium mycotoxins occur worldwide in foods such as cereals and animal forages, leading to acute and chronic exposures in human and animals. Intestinal epithelial cells (IECs) are an important first target site for these dietary toxins. This study investigated the cytotoxicity of four common Fusarium mycotoxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA) and fumonisin B1 (FB1) on a normal porcine jejunal epithelial cell line, IPEC-J2. A dose response relationship between individual mycotoxins and cell viability (MTT assay) was initially investigated, and subsequently cytotoxic and non-cytotoxic concentrations were selected to investigate combinations of two, three and all four of the mycotoxins. For individual mycotoxins, a dose response was observed with cell viability, such that the potency ranking was NIV > DON > ZEA > FB1. At cytotoxic doses of individual mycotoxins, all mixtures gave reduced cell viability compared to control. At noncytotoxic concentrations of individual mycotoxins, all mixtures were cytotoxic with DON-NIV, DON-ZEA, DON-NIV-FB1, DON-ZEA-FB1, NIV-ZEA-FB1 and all four mixed causing the greatest loss of cell viability. The latter observation in particular raises concerns over safety margins based on single toxin species, and suggests that the effects of multiple complex mixtures need to be better understood to assess health risks.
Persistent Identifierhttp://hdl.handle.net/10722/183945
ISSN
2020 Impact Factor: 6.023
2020 SCImago Journal Rankings: 0.951
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWan, LYM-
dc.contributor.authorTurner, PC-
dc.contributor.authorEl-Nezamy, HS-
dc.date.accessioned2013-06-18T04:31:02Z-
dc.date.available2013-06-18T04:31:02Z-
dc.date.issued2013-
dc.identifier.citationFood and Chemical Toxicology, 2013, v. 57, p. 276-283-
dc.identifier.issn0278-6915-
dc.identifier.urihttp://hdl.handle.net/10722/183945-
dc.description.abstractFusarium mycotoxins occur worldwide in foods such as cereals and animal forages, leading to acute and chronic exposures in human and animals. Intestinal epithelial cells (IECs) are an important first target site for these dietary toxins. This study investigated the cytotoxicity of four common Fusarium mycotoxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA) and fumonisin B1 (FB1) on a normal porcine jejunal epithelial cell line, IPEC-J2. A dose response relationship between individual mycotoxins and cell viability (MTT assay) was initially investigated, and subsequently cytotoxic and non-cytotoxic concentrations were selected to investigate combinations of two, three and all four of the mycotoxins. For individual mycotoxins, a dose response was observed with cell viability, such that the potency ranking was NIV > DON > ZEA > FB1. At cytotoxic doses of individual mycotoxins, all mixtures gave reduced cell viability compared to control. At noncytotoxic concentrations of individual mycotoxins, all mixtures were cytotoxic with DON-NIV, DON-ZEA, DON-NIV-FB1, DON-ZEA-FB1, NIV-ZEA-FB1 and all four mixed causing the greatest loss of cell viability. The latter observation in particular raises concerns over safety margins based on single toxin species, and suggests that the effects of multiple complex mixtures need to be better understood to assess health risks.-
dc.languageeng-
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/foodchemtox-
dc.relation.ispartofFood and Chemical Toxicology-
dc.subjectDeoxynivalenol-
dc.subjectNivalenol-
dc.subjectZearalenone-
dc.subjectFumonisin B1-
dc.subjectCytotoxicity-
dc.subjectIntestinal epithelial cells-
dc.titleIndividual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells-
dc.typeArticle-
dc.identifier.emailTurner, PC: pturner3@umd.edu-
dc.identifier.emailEl-Nezamy, HS: elnezami@hkucc.hku.hk-
dc.identifier.authorityEl-Nezamy, HS=rp00694-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fct.2013.03.034-
dc.identifier.pmid23562706-
dc.identifier.scopuseid_2-s2.0-84877112029-
dc.identifier.hkuros214786-
dc.identifier.hkuros248234-
dc.identifier.hkuros248357-
dc.identifier.volume57-
dc.identifier.spage276-
dc.identifier.epage283-
dc.identifier.isiWOS:000320498300035-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0278-6915-

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