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Conference Paper: Pitx2 Overexpression: Effects on the Early Development of Zebrefish Teeth
Title | Pitx2 Overexpression: Effects on the Early Development of Zebrefish Teeth |
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Authors | |
Keywords | Gene expression and Regeneration |
Issue Date | 2013 |
Publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 |
Citation | The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3495 How to Cite? |
Abstract | Objectives:
To address the effects of pitx2 overexpression on the early development of teeth in zebrafish.
Methods:
The mRNA of pitx2 was synthesized in vitro via cloning the CDS of pitx2 by RT-PCR. The DNA fragment was subjected to adding in restriction enzyme sites to become a template. The zebrafish embryos were divided into four experimental groups, with each embryo accepting a microinjection of pitx2 mRNA to establish the overexpression model. Development of embryos within 72hr was observed under microscope. Semi-quantitative RT-PCR and grayscale value analysis were adapted to detect possible changes in the gene expression related to the tooth development.
Results: Morphological observation of 72-hour embryos of zebrafish revealed arrest developments, pericardium edema, notochord deformation, and eye malformation. Semi-quantitative RT-PCR and grayscale value analysis indicated that over-expression of pitx2 down-regulated bmp4, while up-regulated fgf8, dlx2b, and lef1. No significant effect was detected in the expression levels of runx2a.
Conclusions:
Over-expression of pitx2 could result in morphological changes in the embryos of zebrafish. Genes related to early development of teeth of zebrafish were up- or down-regulated, or not effected by pitx2 overexpression. The changes were similar as what we observed in mice. |
Description | Poster Presentation Session 399: Pulp Biology II |
Persistent Identifier | http://hdl.handle.net/10722/183220 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.909 |
DC Field | Value | Language |
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dc.contributor.author | Lu, Q | en_US |
dc.contributor.author | Jin, L | en_US |
dc.date.accessioned | 2013-05-15T01:48:19Z | - |
dc.date.available | 2013-05-15T01:48:19Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3495 | en_US |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | http://hdl.handle.net/10722/183220 | - |
dc.description | Poster Presentation | - |
dc.description | Session 399: Pulp Biology II | - |
dc.description.abstract | Objectives: To address the effects of pitx2 overexpression on the early development of teeth in zebrafish. Methods: The mRNA of pitx2 was synthesized in vitro via cloning the CDS of pitx2 by RT-PCR. The DNA fragment was subjected to adding in restriction enzyme sites to become a template. The zebrafish embryos were divided into four experimental groups, with each embryo accepting a microinjection of pitx2 mRNA to establish the overexpression model. Development of embryos within 72hr was observed under microscope. Semi-quantitative RT-PCR and grayscale value analysis were adapted to detect possible changes in the gene expression related to the tooth development. Results: Morphological observation of 72-hour embryos of zebrafish revealed arrest developments, pericardium edema, notochord deformation, and eye malformation. Semi-quantitative RT-PCR and grayscale value analysis indicated that over-expression of pitx2 down-regulated bmp4, while up-regulated fgf8, dlx2b, and lef1. No significant effect was detected in the expression levels of runx2a. Conclusions: Over-expression of pitx2 could result in morphological changes in the embryos of zebrafish. Genes related to early development of teeth of zebrafish were up- or down-regulated, or not effected by pitx2 overexpression. The changes were similar as what we observed in mice. | - |
dc.language | eng | en_US |
dc.publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 | - |
dc.relation.ispartof | Journal of Dental Research | en_US |
dc.rights | Journal of Dental Research. Copyright © Sage Publications, Inc. | - |
dc.subject | Gene expression and Regeneration | - |
dc.title | Pitx2 Overexpression: Effects on the Early Development of Zebrefish Teeth | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | en_US |
dc.identifier.authority | Jin, L=rp00028 | en_US |
dc.identifier.hkuros | 214399 | en_US |
dc.identifier.volume | 92 | en_US |
dc.identifier.issue | Special Issue A: abstract no. 3495 | en_US |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-0345 | - |