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Article: Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries

TitleAntibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries
Authors
Issue Date2013
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.ann-clinmicrob.com/home/
Citation
Annals of Clinical Microbiology and Antimicrobials, 2013, v. 12, article no. 4 How to Cite?
AbstractBackgrounds: Silver diamine fluoride (SDF) has clinical success in arresting dentin caries, this study aimed to investigate its mechanism of action. Methods: Using a computer-controlled artificial mouth, we studied the effect of 38% SDF on cariogenic biofilms and dentin carious lesions. We used five common cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Actinomyces naeslundii) to form a cariogenic biofilm that generated carious lesions with a depth of approximately 70 um on human dentin blocks. We applied 38% SDF to the lesions in the test group and water to those in the control group. The blocks were incubated in the artificial mouth for 21 days before evaluation. Microbial kinetics, architecture, viability and distribution were evaluated every 7 days using colony forming unit (CFU), scanning electron microscopy and confocal laser scanning microscopy. The physical properties of the carious lesions were evaluated with microhardness testing, energy dispersive spectroscopy (EDS) and Fourier transform infra-red spectroscopy (FTIR). Results: The CFU results revealed fewer colony forming units in the test group compared with the control group (p < 0.01). Scanning electron microscopy and confocal microscopy showed less bacterial growth in the test group, and confluent cariogenic biofilm in the control group (p < 0.01). The microhardness and weight percentages of calcium and phosphorus in the test group from the outermost 50mum were higher than in the control group (p < 0.05). EDS showed that calcium and phosphous were higher in outer 50 mum in test groups than in the control FTIR revealed less exposed collagen I in the test lesions compared with the control group (p < 0.01). Conclusions: 38% SDF inhibits multi-species cariogenic biofilm formation on dentin carious lesions and reduces the demineralization process.
Persistent Identifierhttp://hdl.handle.net/10722/181971
ISSN
2021 Impact Factor: 6.781
2020 SCImago Journal Rankings: 1.194
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMei, L-
dc.contributor.authorLi, QL-
dc.contributor.authorChu, CH-
dc.contributor.authorLo, ECM-
dc.contributor.authorSamaranayake, LP-
dc.date.accessioned2013-04-17T07:15:31Z-
dc.date.available2013-04-17T07:15:31Z-
dc.date.issued2013-
dc.identifier.citationAnnals of Clinical Microbiology and Antimicrobials, 2013, v. 12, article no. 4-
dc.identifier.issn1476-0711-
dc.identifier.urihttp://hdl.handle.net/10722/181971-
dc.description.abstractBackgrounds: Silver diamine fluoride (SDF) has clinical success in arresting dentin caries, this study aimed to investigate its mechanism of action. Methods: Using a computer-controlled artificial mouth, we studied the effect of 38% SDF on cariogenic biofilms and dentin carious lesions. We used five common cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Actinomyces naeslundii) to form a cariogenic biofilm that generated carious lesions with a depth of approximately 70 um on human dentin blocks. We applied 38% SDF to the lesions in the test group and water to those in the control group. The blocks were incubated in the artificial mouth for 21 days before evaluation. Microbial kinetics, architecture, viability and distribution were evaluated every 7 days using colony forming unit (CFU), scanning electron microscopy and confocal laser scanning microscopy. The physical properties of the carious lesions were evaluated with microhardness testing, energy dispersive spectroscopy (EDS) and Fourier transform infra-red spectroscopy (FTIR). Results: The CFU results revealed fewer colony forming units in the test group compared with the control group (p < 0.01). Scanning electron microscopy and confocal microscopy showed less bacterial growth in the test group, and confluent cariogenic biofilm in the control group (p < 0.01). The microhardness and weight percentages of calcium and phosphorus in the test group from the outermost 50mum were higher than in the control group (p < 0.05). EDS showed that calcium and phosphous were higher in outer 50 mum in test groups than in the control FTIR revealed less exposed collagen I in the test lesions compared with the control group (p < 0.01). Conclusions: 38% SDF inhibits multi-species cariogenic biofilm formation on dentin carious lesions and reduces the demineralization process.-
dc.languageeng-
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.ann-clinmicrob.com/home/-
dc.relation.ispartofAnnals of Clinical Microbiology and Antimicrobials-
dc.rightsAnnals of Clinical Microbiology and Antimicrobials. Copyright © BioMed Central Ltd.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleAntibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries-
dc.typeArticle-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1476-0711&volume=12&issue=4&spage=1&epage=7&date=2013&atitle=Antibacterial+effects+of+silver+diamine+fluoride+on+multi-species+cariogenic+biofilm+on+cariesen_US
dc.identifier.emailMei, L: leimei@hkusua.hku.hk-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hk-
dc.identifier.emailSamaranayake, LP: lakshman@hku.hk-
dc.identifier.authorityMei, L=rp01840-
dc.identifier.authorityChu, CH=rp00022-
dc.identifier.authorityLo, ECM=rp00015-
dc.identifier.authoritySamaranayake, LP=rp00023-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1476-0711-12-4-
dc.identifier.pmid23442825-
dc.identifier.pmcidPMC3599989-
dc.identifier.scopuseid_2-s2.0-84874214463-
dc.identifier.hkuros213952-
dc.identifier.volume12-
dc.identifier.spagearticle no. 4-
dc.identifier.epagearticle no. 4-
dc.identifier.isiWOS:000316174200001-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1476-0711-

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