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- PMID: 15593292
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Article: Insect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenes
Title | Insect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenes |
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Authors | |
Keywords | Cnaphalocrocis medinalis Gna gene Inheritance Insect resistance Nilapavata lugens Sbti gene Transgenic rice |
Issue Date | 2005 |
Publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/pestmanagementscience |
Citation | Pest Management Science, 2005, v. 61 n. 4, p. 390-396 How to Cite? |
Abstract | Molecular genetic analysis and insect bioassay of transgenic indica rice 'Zhuxian B' plants carrying snowdrop lectin gene (gna) and soybean trypsin inhibitor gene (sbti) were investigated in detail. PCR, 'dot' blot and PCR-Southern blot analysis showed that both transgenes had been incorporated into the rice genome and transmitted up to R3 progeny in most lines tested. Some transgenic lines exhibited Mendelian segregation, but the other showed either 1:1 (positive: negative for the transgenes) or other aberrant segregation patterns. The segregation patterns of gna gene crossed between R2 and R3 progeny. In half of transgenic R3 lines, gna and sbti transgenes co-segregated. Two independent homozygous lines expressing double transgenes were identified in R3 progeny. Southern blot analysis demonstrated that the copy numbers of integrated gna and sbti transgenes varied from one to ten in different lines. Insect bioassay data showed that most transgenic plants had better resistance to both Nilaparvata lugens (Ståhl) and Cnaphalocrocis medinalis (Guenée) than wild-type plants. The insect resistance of transgenic lines increased with the increase in transgene positive ratio in most of the transgenic lines. In all, we obtained nine lines of R3 transgenic plants, including one pure line, which had better resistance to both N lugens and C medinalis than wild-type plants. © 2004 Society of Chemical Industry. |
Persistent Identifier | http://hdl.handle.net/10722/179781 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.950 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, G | en_US |
dc.contributor.author | Xu, X | en_US |
dc.contributor.author | Xing, H | en_US |
dc.contributor.author | Zhu, H | en_US |
dc.contributor.author | Fan, Q | en_US |
dc.date.accessioned | 2012-12-19T10:04:33Z | - |
dc.date.available | 2012-12-19T10:04:33Z | - |
dc.date.issued | 2005 | en_US |
dc.identifier.citation | Pest Management Science, 2005, v. 61 n. 4, p. 390-396 | en_US |
dc.identifier.issn | 1526-498X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179781 | - |
dc.description.abstract | Molecular genetic analysis and insect bioassay of transgenic indica rice 'Zhuxian B' plants carrying snowdrop lectin gene (gna) and soybean trypsin inhibitor gene (sbti) were investigated in detail. PCR, 'dot' blot and PCR-Southern blot analysis showed that both transgenes had been incorporated into the rice genome and transmitted up to R3 progeny in most lines tested. Some transgenic lines exhibited Mendelian segregation, but the other showed either 1:1 (positive: negative for the transgenes) or other aberrant segregation patterns. The segregation patterns of gna gene crossed between R2 and R3 progeny. In half of transgenic R3 lines, gna and sbti transgenes co-segregated. Two independent homozygous lines expressing double transgenes were identified in R3 progeny. Southern blot analysis demonstrated that the copy numbers of integrated gna and sbti transgenes varied from one to ten in different lines. Insect bioassay data showed that most transgenic plants had better resistance to both Nilaparvata lugens (Ståhl) and Cnaphalocrocis medinalis (Guenée) than wild-type plants. The insect resistance of transgenic lines increased with the increase in transgene positive ratio in most of the transgenic lines. In all, we obtained nine lines of R3 transgenic plants, including one pure line, which had better resistance to both N lugens and C medinalis than wild-type plants. © 2004 Society of Chemical Industry. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/pestmanagementscience | en_US |
dc.relation.ispartof | Pest Management Science | en_US |
dc.subject | Cnaphalocrocis medinalis | - |
dc.subject | Gna gene | - |
dc.subject | Inheritance | - |
dc.subject | Insect resistance | - |
dc.subject | Nilapavata lugens | - |
dc.subject | Sbti gene | - |
dc.subject | Transgenic rice | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Galanthus - Genetics | en_US |
dc.subject.mesh | Gene Expression | en_US |
dc.subject.mesh | Hemiptera | en_US |
dc.subject.mesh | Insects | en_US |
dc.subject.mesh | Mannose-Binding Lectins - Genetics - Metabolism | en_US |
dc.subject.mesh | Oryza Sativa - Genetics - Parasitology | en_US |
dc.subject.mesh | Plant Lectins - Genetics - Metabolism | en_US |
dc.subject.mesh | Plants, Genetically Modified - Parasitology | en_US |
dc.subject.mesh | Soybeans - Genetics | en_US |
dc.subject.mesh | Transgenes | en_US |
dc.subject.mesh | Trypsin Inhibitors - Genetics - Metabolism | en_US |
dc.title | Insect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Zhu, H: zhuhch@hku.hk | en_US |
dc.identifier.authority | Zhu, H=rp01535 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/ps.990 | en_US |
dc.identifier.pmid | 15593292 | - |
dc.identifier.scopus | eid_2-s2.0-16244394834 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-16244394834&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 61 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 390 | en_US |
dc.identifier.epage | 396 | en_US |
dc.identifier.isi | WOS:000228017000008 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Li, G=7407051300 | en_US |
dc.identifier.scopusauthorid | Xu, X=35228207200 | en_US |
dc.identifier.scopusauthorid | Xing, H=8519673700 | en_US |
dc.identifier.scopusauthorid | Zhu, H=25724029300 | en_US |
dc.identifier.scopusauthorid | Fan, Q=36098887700 | en_US |
dc.identifier.citeulike | 130508 | - |
dc.identifier.issnl | 1526-498X | - |