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Article: Polyadenylation of influenza virus mRNA transcribed in vitro from model virion RNA templates: Requirement for 5' conserved sequences

TitlePolyadenylation of influenza virus mRNA transcribed in vitro from model virion RNA templates: Requirement for 5' conserved sequences
Authors
Issue Date1998
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 1998, v. 72 n. 2, p. 1280-1286 How to Cite?
AbstractHere we report the development of two independent assays which demonstrate for the first time that exogenous model RNA templates based on influenza virus virion RNA (vRNA) are transcribed in vitro to produce polyadenylated mRNA. We investigated the activities of mutated templates with known polymerase binding properties to test our model that polyadenylation occurs when a polymerase complex, which is bound to conserved 5' sequences of vRNA, prevents read-through of the U track at which polyadenylation subsequently occurs by reiterative copying. Mutated templates with perturbed polymerase binding sites (i.e., a deletion mutant lacking the first 4 5' residues and a U→A point mutant at the third residue) initiated transcription in the in vitro assay but failed to produce polyadenylated transcripts, whereas an A→U point mutant at the fourth residue, which retained polymerase binding properties similar to those of the wild type, produced polyadenylated transcripts. Our results show that nucleotides within the conserved 5' sequence are required for polyadenylation and support the hypothesis that polymerase binding to 5' sequences of the template is required for mRNA synthesis.
Persistent Identifierhttp://hdl.handle.net/10722/179760
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.378
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPritlove, DCen_US
dc.contributor.authorPoon, LLMen_US
dc.contributor.authorFodor, Een_US
dc.contributor.authorSharps, Jen_US
dc.contributor.authorBrownlee, GGen_US
dc.date.accessioned2012-12-19T10:04:23Z-
dc.date.available2012-12-19T10:04:23Z-
dc.date.issued1998en_US
dc.identifier.citationJournal Of Virology, 1998, v. 72 n. 2, p. 1280-1286en_US
dc.identifier.issn0022-538Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179760-
dc.description.abstractHere we report the development of two independent assays which demonstrate for the first time that exogenous model RNA templates based on influenza virus virion RNA (vRNA) are transcribed in vitro to produce polyadenylated mRNA. We investigated the activities of mutated templates with known polymerase binding properties to test our model that polyadenylation occurs when a polymerase complex, which is bound to conserved 5' sequences of vRNA, prevents read-through of the U track at which polyadenylation subsequently occurs by reiterative copying. Mutated templates with perturbed polymerase binding sites (i.e., a deletion mutant lacking the first 4 5' residues and a U→A point mutant at the third residue) initiated transcription in the in vitro assay but failed to produce polyadenylated transcripts, whereas an A→U point mutant at the fourth residue, which retained polymerase binding properties similar to those of the wild type, produced polyadenylated transcripts. Our results show that nucleotides within the conserved 5' sequence are required for polyadenylation and support the hypothesis that polymerase binding to 5' sequences of the template is required for mRNA synthesis.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_US
dc.relation.ispartofJournal of Virologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshConserved Sequenceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOrthomyxoviridae - Geneticsen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshRna, Viral - Geneticsen_US
dc.subject.meshTemplates, Geneticen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshVirion - Geneticsen_US
dc.titlePolyadenylation of influenza virus mRNA transcribed in vitro from model virion RNA templates: Requirement for 5' conserved sequencesen_US
dc.typeArticleen_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/JVI.72.2.1280-1286.1998-
dc.identifier.pmid9445028-
dc.identifier.scopuseid_2-s2.0-0031883261en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031883261&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume72en_US
dc.identifier.issue2en_US
dc.identifier.spage1280en_US
dc.identifier.epage1286en_US
dc.identifier.isiWOS:000071440200049-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPritlove, DC=6602418386en_US
dc.identifier.scopusauthoridPoon, LLM=7005441747en_US
dc.identifier.scopusauthoridFodor, E=7006539874en_US
dc.identifier.scopusauthoridSharps, J=6602899431en_US
dc.identifier.scopusauthoridBrownlee, GG=35994351900en_US
dc.identifier.issnl0022-538X-

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