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- Publisher Website: 10.1017/S0950268800068114
- Scopus: eid_2-s2.0-0027323901
- PMID: 8472764
- WOS: WOS:A1993KX49900002
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Article: A longitudinal study of vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka
Title | A longitudinal study of vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka |
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Authors | |
Issue Date | 1993 |
Publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=HYG |
Citation | Epidemiology And Infection, 1993, v. 110 n. 2, p. 197-208 How to Cite? |
Abstract | Two cohorts of 10 and 16 calves were followed at weekly or fortnightly intervals from 4-28 and 1-9 weeks respectively to determine whether natural infection by Vero cytotoxin (VT) producing Escherichia coli (VTEC) occurred. Ninety-one of 171 (53%) faecal specimens were VTEC positive and 20-80% of animals at any given time excreted VTEC. Of 104 VTEC strains studies further, 6 different serogroups (O 22.H16; O 25.H5; O 49.H-; O 86.H26; O 88.H25; O 153.H12) and an untypable strain (O @?.H21) were identified. All strains belonging to the same serotype had identical profiles of reactivity with DNA probes to toxins VT1 or 2. LTI or II and a probe (CVD419) derived from a plasmid carried by entero-haemorrhagic Escherichia coli O 157.H7. Four of these serotypes were found in the faecal flora of the calves, taken as a group, throughout the 4-month study period. Sixty percent of the strains hybridized with the probe for VT1, 4% with the probe for VT2, and 36% with both probes. Faecal VTEC were significantly associated with overt diarrhoeal illness in animals < 10 weeks of age, but no characterisitic profile of markers (serotype or hybridization pattern) in E. coli isolates was associated with diarrhoea. A serological response to VT1 was detected in some animals, but faecal VT1 VTEC excretion persisted in spite of seroconversion. VT1 seroconversion was not associated with diarrhoea. A serological response to VT2 was not detected even in those animals excreting VT2 VTEC in the faeces. |
Persistent Identifier | http://hdl.handle.net/10722/179747 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.830 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Tokhi, AM | en_US |
dc.contributor.author | Peiris, JSM | en_US |
dc.contributor.author | Scotland, SM | en_US |
dc.contributor.author | Willshaw, GA | en_US |
dc.contributor.author | Smith, HR | en_US |
dc.contributor.author | Cheasty, T | en_US |
dc.date.accessioned | 2012-12-19T10:04:16Z | - |
dc.date.available | 2012-12-19T10:04:16Z | - |
dc.date.issued | 1993 | en_US |
dc.identifier.citation | Epidemiology And Infection, 1993, v. 110 n. 2, p. 197-208 | en_US |
dc.identifier.issn | 0950-2688 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179747 | - |
dc.description.abstract | Two cohorts of 10 and 16 calves were followed at weekly or fortnightly intervals from 4-28 and 1-9 weeks respectively to determine whether natural infection by Vero cytotoxin (VT) producing Escherichia coli (VTEC) occurred. Ninety-one of 171 (53%) faecal specimens were VTEC positive and 20-80% of animals at any given time excreted VTEC. Of 104 VTEC strains studies further, 6 different serogroups (O 22.H16; O 25.H5; O 49.H-; O 86.H26; O 88.H25; O 153.H12) and an untypable strain (O @?.H21) were identified. All strains belonging to the same serotype had identical profiles of reactivity with DNA probes to toxins VT1 or 2. LTI or II and a probe (CVD419) derived from a plasmid carried by entero-haemorrhagic Escherichia coli O 157.H7. Four of these serotypes were found in the faecal flora of the calves, taken as a group, throughout the 4-month study period. Sixty percent of the strains hybridized with the probe for VT1, 4% with the probe for VT2, and 36% with both probes. Faecal VTEC were significantly associated with overt diarrhoeal illness in animals < 10 weeks of age, but no characterisitic profile of markers (serotype or hybridization pattern) in E. coli isolates was associated with diarrhoea. A serological response to VT1 was detected in some animals, but faecal VT1 VTEC excretion persisted in spite of seroconversion. VT1 seroconversion was not associated with diarrhoea. A serological response to VT2 was not detected even in those animals excreting VT2 VTEC in the faeces. | en_US |
dc.language | eng | en_US |
dc.publisher | Cambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=HYG | en_US |
dc.relation.ispartof | Epidemiology and Infection | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Bacterial - Immunology | en_US |
dc.subject.mesh | Bacterial Toxins - Biosynthesis - Immunology | en_US |
dc.subject.mesh | Cattle | en_US |
dc.subject.mesh | Cattle Diseases - Epidemiology - Microbiology | en_US |
dc.subject.mesh | Cytotoxins - Biosynthesis - Immunology | en_US |
dc.subject.mesh | Dna - Genetics | en_US |
dc.subject.mesh | Dna Probes | en_US |
dc.subject.mesh | Diarrhea - Epidemiology - Veterinary | en_US |
dc.subject.mesh | Enterotoxins - Biosynthesis - Immunology | en_US |
dc.subject.mesh | Escherichia Coli - Genetics - Immunology - Metabolism | en_US |
dc.subject.mesh | Escherichia Coli Infections - Epidemiology - Veterinary | en_US |
dc.subject.mesh | Feces - Microbiology | en_US |
dc.subject.mesh | Longitudinal Studies | en_US |
dc.subject.mesh | Nucleic Acid Hybridization | en_US |
dc.subject.mesh | Phenotype | en_US |
dc.subject.mesh | Prevalence | en_US |
dc.subject.mesh | Shiga Toxin 1 | en_US |
dc.subject.mesh | Sri Lanka - Epidemiology | en_US |
dc.subject.mesh | Virulence | en_US |
dc.title | A longitudinal study of vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka | en_US |
dc.type | Article | en_US |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_US |
dc.identifier.authority | Peiris, JSM=rp00410 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1017/S0950268800068114 | - |
dc.identifier.pmid | 8472764 | - |
dc.identifier.scopus | eid_2-s2.0-0027323901 | en_US |
dc.identifier.volume | 110 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 197 | en_US |
dc.identifier.epage | 208 | en_US |
dc.identifier.isi | WOS:A1993KX49900002 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Tokhi, AM=6506577935 | en_US |
dc.identifier.scopusauthorid | Peiris, JSM=7005486823 | en_US |
dc.identifier.scopusauthorid | Scotland, SM=7004747820 | en_US |
dc.identifier.scopusauthorid | Willshaw, GA=7005289886 | en_US |
dc.identifier.scopusauthorid | Smith, HR=10339051700 | en_US |
dc.identifier.scopusauthorid | Cheasty, T=7007147387 | en_US |
dc.identifier.issnl | 0950-2688 | - |