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- Publisher Website: 10.1038/289189a0
- Scopus: eid_2-s2.0-0019831472
- PMID: 7453820
- WOS: WOS:A1981KY74600048
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Article: Monoclonal-anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages
Title | Monoclonal-anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages |
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Authors | |
Issue Date | 1981 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/nature |
Citation | Nature, 1981, v. 289 n. 5794, p. 189-191 How to Cite? |
Abstract | Flaviviruses, when complexed with antibody at subneutralizing concentrations, show enhanced replication in human and simian peripheral blood leukocytes and in P388 D1 and other macrophage cell lines. A comparable phenomenon has been demonstrated with alphaviruses and Bunyaviruses in P388 D1 cells, but cells lacking macrophage characteristics fail to show antibody-dependent enhancement (ADE) of viral replication. It has been suggested that the macrophage Fc receptor (FcR) provides an efficient route of entry of virus through the attachment of non-neutralized virus-antibody complexes and that for those viruses that escape destruction by the phagocyte, antibody results in a paradoxical increase in virus replication. Wst Nile virus (WNV) replication in the P388 D1 macrophage cell line provides a reproducible model system for studying ADE of viral replication. Mouse macrophages have two FcRs-FcR1, which is trypsin-sensitive and binds IgG2a, and FcRII, which is trypsin-resistant and binds IgG2b and IgG1 complexes. The FcR has been purified using rat anti-mouse FcR monoclonal antibody which blocks FcRII. We show here that anti-FcR IgG and its Fab fragment block ADE of virus replication by anti-WNV monoclonal antibodies. |
Persistent Identifier | http://hdl.handle.net/10722/179728 |
ISSN | 2023 Impact Factor: 50.5 2023 SCImago Journal Rankings: 18.509 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Peiris, JSM | en_US |
dc.contributor.author | Gordon, S | en_US |
dc.contributor.author | Unkeless, JC | en_US |
dc.contributor.author | Porterfield, JS | en_US |
dc.date.accessioned | 2012-12-19T10:04:06Z | - |
dc.date.available | 2012-12-19T10:04:06Z | - |
dc.date.issued | 1981 | en_US |
dc.identifier.citation | Nature, 1981, v. 289 n. 5794, p. 189-191 | en_US |
dc.identifier.issn | 0028-0836 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179728 | - |
dc.description.abstract | Flaviviruses, when complexed with antibody at subneutralizing concentrations, show enhanced replication in human and simian peripheral blood leukocytes and in P388 D1 and other macrophage cell lines. A comparable phenomenon has been demonstrated with alphaviruses and Bunyaviruses in P388 D1 cells, but cells lacking macrophage characteristics fail to show antibody-dependent enhancement (ADE) of viral replication. It has been suggested that the macrophage Fc receptor (FcR) provides an efficient route of entry of virus through the attachment of non-neutralized virus-antibody complexes and that for those viruses that escape destruction by the phagocyte, antibody results in a paradoxical increase in virus replication. Wst Nile virus (WNV) replication in the P388 D1 macrophage cell line provides a reproducible model system for studying ADE of viral replication. Mouse macrophages have two FcRs-FcR1, which is trypsin-sensitive and binds IgG2a, and FcRII, which is trypsin-resistant and binds IgG2b and IgG1 complexes. The FcR has been purified using rat anti-mouse FcR monoclonal antibody which blocks FcRII. We show here that anti-FcR IgG and its Fab fragment block ADE of virus replication by anti-WNV monoclonal antibodies. | en_US |
dc.language | eng | en_US |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/nature | en_US |
dc.relation.ispartof | Nature | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Viral | en_US |
dc.subject.mesh | Antigen-Antibody Complex | en_US |
dc.subject.mesh | Antigens, Viral | en_US |
dc.subject.mesh | Clone Cells - Immunology | en_US |
dc.subject.mesh | Immunoglobulin Fab Fragments | en_US |
dc.subject.mesh | Immunoglobulin G | en_US |
dc.subject.mesh | Macrophages - Immunology - Microbiology | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Receptors, Fc - Metabolism | en_US |
dc.subject.mesh | Virus Replication | en_US |
dc.title | Monoclonal-anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages | en_US |
dc.type | Article | en_US |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_US |
dc.identifier.authority | Peiris, JSM=rp00410 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1038/289189a0 | - |
dc.identifier.pmid | 7453820 | - |
dc.identifier.scopus | eid_2-s2.0-0019831472 | en_US |
dc.identifier.volume | 289 | en_US |
dc.identifier.issue | 5794 | en_US |
dc.identifier.spage | 189 | en_US |
dc.identifier.epage | 191 | en_US |
dc.identifier.isi | WOS:A1981KY74600048 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Peiris, JSM=7005486823 | en_US |
dc.identifier.scopusauthorid | Gordon, S=35391350600 | en_US |
dc.identifier.scopusauthorid | Unkeless, JC=7006626093 | en_US |
dc.identifier.scopusauthorid | Porterfield, JS=7005608715 | en_US |
dc.identifier.issnl | 0028-0836 | - |