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- Publisher Website: 10.1007/s00216-005-0062-1
- Scopus: eid_2-s2.0-27744522318
- PMID: 16172881
- WOS: WOS:000233874900026
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Conference Paper: Determination of vitamin B 1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection
Title | Determination of vitamin B 1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection |
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Authors | |
Keywords | Fermentation Media High-Performance Liquid Chromatography Seawater Solid-Phase Extraction Vitamin B 1 |
Issue Date | 2005 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm |
Citation | Analytical And Bioanalytical Chemistry, 2005, v. 383 n. 5, p. 875-879 How to Cite? |
Abstract | A highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed for determination of vitamin B 1. Vitamin B 1 was converted into a fluorescent compound by treatment with hydrogen peroxide-horseradish peroxidase and the derivative was subsequently analyzed by HPLC on a Waters Spherisorb ODS2 column (250 mm×4.6 mm ID, 5 μm) with 40:60 methanol-pH 8.5 acetate buffer solution as mobile phase and fluorescence detection at 440 nm (with excitation at 375 nm). The calibration graph was linear from 5.00×10 -10 mol L -1 to 5.00×10 -7 mol L -1 for vitamin B 1 with a correlation coefficient of 0.9991 (n=9). The detection limit was 1.0×10 -10 mol L -1. The method was successfully used for determination of vitamin B 1 at pg mL -1 levels in microalgal fermentation media and seawater after solid-phase extraction. Recovery was from 89 to 110% and the relative standard deviation was in the range 1.1 to 4.3%. © Springer-Verlag 2005. |
Persistent Identifier | http://hdl.handle.net/10722/179589 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.686 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | He, HZ | en_US |
dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T10:00:03Z | - |
dc.date.available | 2012-12-19T10:00:03Z | - |
dc.date.issued | 2005 | en_US |
dc.identifier.citation | Analytical And Bioanalytical Chemistry, 2005, v. 383 n. 5, p. 875-879 | en_US |
dc.identifier.issn | 1618-2642 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179589 | - |
dc.description.abstract | A highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed for determination of vitamin B 1. Vitamin B 1 was converted into a fluorescent compound by treatment with hydrogen peroxide-horseradish peroxidase and the derivative was subsequently analyzed by HPLC on a Waters Spherisorb ODS2 column (250 mm×4.6 mm ID, 5 μm) with 40:60 methanol-pH 8.5 acetate buffer solution as mobile phase and fluorescence detection at 440 nm (with excitation at 375 nm). The calibration graph was linear from 5.00×10 -10 mol L -1 to 5.00×10 -7 mol L -1 for vitamin B 1 with a correlation coefficient of 0.9991 (n=9). The detection limit was 1.0×10 -10 mol L -1. The method was successfully used for determination of vitamin B 1 at pg mL -1 levels in microalgal fermentation media and seawater after solid-phase extraction. Recovery was from 89 to 110% and the relative standard deviation was in the range 1.1 to 4.3%. © Springer-Verlag 2005. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm | en_US |
dc.relation.ispartof | Analytical and Bioanalytical Chemistry | en_US |
dc.subject | Fermentation Media | en_US |
dc.subject | High-Performance Liquid Chromatography | en_US |
dc.subject | Seawater | en_US |
dc.subject | Solid-Phase Extraction | en_US |
dc.subject | Vitamin B 1 | en_US |
dc.title | Determination of vitamin B 1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/s00216-005-0062-1 | en_US |
dc.identifier.pmid | 16172881 | - |
dc.identifier.scopus | eid_2-s2.0-27744522318 | en_US |
dc.identifier.hkuros | 122501 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-27744522318&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 383 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 875 | en_US |
dc.identifier.epage | 879 | en_US |
dc.identifier.isi | WOS:000233874900026 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | He, HZ=55214936100 | en_US |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 1618-2642 | - |