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Article: Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism

TitleDifferentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism
Authors
Fu, RZWang, JZhang, YBWang, ZTBut, PPHLi, NShaw, PC
KeywordsC. modesta
C. pilosula
C. tangshen
Campanulaceae
Campanumoea javania
Chinese medicine
Codonopsis
Dangshen
Internal transcribed spacer (ITS)
PCR
Platycodon grandiflorus
RDNA
RFLP
Issue Date1999
PublisherGeorg Thieme Verlag. The Journal's web site is located at http://www.thieme.de/plantamedica/index.html
Citation
Planta Medica, 1999, v. 65 n. 7, p. 648-650 How to Cite?
DOI: http://dx.doi.org/10.1055/s-1999-14091
AbstractDNA sequence analysis of rDNA internal transcribed spacer (ITS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were exploited for their applications in differentiating medicinal species Codonopsis pilosula, C. tangshen, C. modesta, and C. nervosa var. macrantha, from two related adulterants Campanumoea javania and Platycodon grandiflorus. The data demonstrated that the rDNA ITSI and ITSII sequences of the four Codonopsis are highly homologous but not identical, and are significantly different from those of the two adulterants. The sequence difference allows effective and reliable differentiation of Codonopsis from the adulterants by PCR-RFLP.
Persistent Identifierhttp://hdl.handle.net/10722/179403
ISSN
0032-0943
2023 Impact Factor: 2.1
2023 SCImago Journal Rankings: 0.445
ISI Accession Number ID
WOS:000083635300013
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorFu, RZen_US
dc.contributor.authorWang, Jen_US
dc.contributor.authorZhang, YBen_US
dc.contributor.authorWang, ZTen_US
dc.contributor.authorBut, PPHen_US
dc.contributor.authorLi, Nen_US
dc.contributor.authorShaw, PCen_US
dc.date.accessioned2012-12-19T09:56:12Z-
dc.date.available2012-12-19T09:56:12Z-
dc.date.issued1999en_US
dc.identifier.citationPlanta Medica, 1999, v. 65 n. 7, p. 648-650en_US
dc.identifier.issn0032-0943en_US
dc.identifier.urihttp://hdl.handle.net/10722/179403-
dc.description.abstractDNA sequence analysis of rDNA internal transcribed spacer (ITS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were exploited for their applications in differentiating medicinal species Codonopsis pilosula, C. tangshen, C. modesta, and C. nervosa var. macrantha, from two related adulterants Campanumoea javania and Platycodon grandiflorus. The data demonstrated that the rDNA ITSI and ITSII sequences of the four Codonopsis are highly homologous but not identical, and are significantly different from those of the two adulterants. The sequence difference allows effective and reliable differentiation of Codonopsis from the adulterants by PCR-RFLP.en_US
dc.languageengen_US
dc.publisherGeorg Thieme Verlag. The Journal's web site is located at http://www.thieme.de/plantamedica/index.htmlen_US
dc.relation.ispartofPlanta Medicaen_US
dc.subjectC. modesta-
dc.subjectC. pilosula-
dc.subjectC. tangshen-
dc.subjectCampanulaceae-
dc.subjectCampanumoea javania-
dc.subjectChinese medicine-
dc.subjectCodonopsis-
dc.subjectDangshen-
dc.subjectInternal transcribed spacer (ITS)-
dc.subjectPCR-
dc.subjectPlatycodon grandiflorus-
dc.subjectRDNA-
dc.subjectRFLP-
dc.subject.meshAsteraceae - Chemistry - Geneticsen_US
dc.subject.meshDna, Ribosomal - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMedicine, Chinese Traditionalen_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshPlant Roots - Geneticsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshRna, Plant - Geneticsen_US
dc.titleDifferentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphismen_US
dc.typeArticleen_US
dc.identifier.emailZhang, YB: ybzhang@hku.hken_US
dc.identifier.authorityZhang, YB=rp01410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1055/s-1999-14091en_US
dc.identifier.pmid10575378-
dc.identifier.scopuseid_2-s2.0-0032739778en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032739778&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume65en_US
dc.identifier.issue7en_US
dc.identifier.spage648en_US
dc.identifier.epage650en_US
dc.identifier.isiWOS:000083635300013-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridFu, RZ=7203054356en_US
dc.identifier.scopusauthoridWang, J=9637013000en_US
dc.identifier.scopusauthoridZhang, YB=23483121900en_US
dc.identifier.scopusauthoridWang, ZT=22836797600en_US
dc.identifier.scopusauthoridBut, PPH=7005593430en_US
dc.identifier.scopusauthoridLi, N=7403609459en_US
dc.identifier.scopusauthoridShaw, PC=35599523600en_US
dc.identifier.issnl0032-0943-

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