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Article: Overexpression of arabidopsis acbp3 enhances npr1-dependent plant resistance to pseudomonas syringe pv tomato dc3000
Title | Overexpression of arabidopsis acbp3 enhances npr1-dependent plant resistance to pseudomonas syringe pv tomato dc3000 |
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Authors | |
Issue Date | 2011 |
Publisher | American Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org |
Citation | Plant Physiology, 2011, v. 156 n. 4, p. 2069-2081 How to Cite? |
Abstract | ACBP3 is one of six Arabidopsis (Arabidopsis thaliana) genes, designated ACBP1 to ACBP6, that encode acyl-coenzyme A (CoA)-binding proteins (ACBPs). These ACBPs bind long-chain acyl-CoA esters and phospholipids and are involved in diverse cellular functions, including acyl-CoA homeostasis, development, and stress tolerance. Recombinant ACBP3 binds polyunsaturated acyl-CoA esters and phospholipids in vitro. Here, we show that ACBP3 plays a role in the plant defense response to the bacterial pathogen Pseudomonas syringae pv tomato DC3000. ACBP3 mRNA was up-regulated upon pathogen infection and treatments using pathogen elicitors and defense-related phytohormones. Transgenic Arabidopsis ACBP3 overexpressors (ACBP3-OEs) showed constitutive expression of pathogenesis-related genes (PR1, PR2, and PR5), cell death, and hydrogen peroxide accumulation in leaves. Consequently, ACBP3-OEs displayed enhanced resistance to the bacterial pathogen P. syringae DC3000. In contrast, the acbp3 T-DNA insertional mutant was more susceptible and exhibited lower PR gene transcript levels upon infection. Using the ACBP3 OE-1 line in combination with nonexpressor of PR genes1 (npr1-5) or coronatine-insensitive1 (coi1-2), we concluded that the enhanced PR gene expression and P. syringae DC3000 resistance in the ACBP3-OEs are dependent on the NPR1-mediated, but not the COI1-mediated, signaling pathway. Given that ACBP3-OEs showed greater susceptibility to infection by the necrotrophic fungus Botrytis cinerea while the acbp3 mutant was less susceptible, we suggest that ACBP3 plays a role in the plant defense response against biotrophic pathogens that is distinct from necrotrophic pathogens. ACBP3 function in plant defense was supported further by bioinformatics data showing up-regulation of many biotic and abiotic stress-related genes in ACBP3 OE-1 in comparison with the wild type. © 2011 American Society of Plant Biologists. |
Persistent Identifier | http://hdl.handle.net/10722/179248 |
ISSN | 2023 Impact Factor: 6.5 2023 SCImago Journal Rankings: 2.101 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xiao, S | en_US |
dc.contributor.author | Chye, ML | en_US |
dc.date.accessioned | 2012-12-19T09:53:22Z | - |
dc.date.available | 2012-12-19T09:53:22Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Plant Physiology, 2011, v. 156 n. 4, p. 2069-2081 | en_US |
dc.identifier.issn | 0032-0889 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179248 | - |
dc.description.abstract | ACBP3 is one of six Arabidopsis (Arabidopsis thaliana) genes, designated ACBP1 to ACBP6, that encode acyl-coenzyme A (CoA)-binding proteins (ACBPs). These ACBPs bind long-chain acyl-CoA esters and phospholipids and are involved in diverse cellular functions, including acyl-CoA homeostasis, development, and stress tolerance. Recombinant ACBP3 binds polyunsaturated acyl-CoA esters and phospholipids in vitro. Here, we show that ACBP3 plays a role in the plant defense response to the bacterial pathogen Pseudomonas syringae pv tomato DC3000. ACBP3 mRNA was up-regulated upon pathogen infection and treatments using pathogen elicitors and defense-related phytohormones. Transgenic Arabidopsis ACBP3 overexpressors (ACBP3-OEs) showed constitutive expression of pathogenesis-related genes (PR1, PR2, and PR5), cell death, and hydrogen peroxide accumulation in leaves. Consequently, ACBP3-OEs displayed enhanced resistance to the bacterial pathogen P. syringae DC3000. In contrast, the acbp3 T-DNA insertional mutant was more susceptible and exhibited lower PR gene transcript levels upon infection. Using the ACBP3 OE-1 line in combination with nonexpressor of PR genes1 (npr1-5) or coronatine-insensitive1 (coi1-2), we concluded that the enhanced PR gene expression and P. syringae DC3000 resistance in the ACBP3-OEs are dependent on the NPR1-mediated, but not the COI1-mediated, signaling pathway. Given that ACBP3-OEs showed greater susceptibility to infection by the necrotrophic fungus Botrytis cinerea while the acbp3 mutant was less susceptible, we suggest that ACBP3 plays a role in the plant defense response against biotrophic pathogens that is distinct from necrotrophic pathogens. ACBP3 function in plant defense was supported further by bioinformatics data showing up-regulation of many biotic and abiotic stress-related genes in ACBP3 OE-1 in comparison with the wild type. © 2011 American Society of Plant Biologists. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org | en_US |
dc.relation.ispartof | Plant Physiology | en_US |
dc.subject.mesh | Arabidopsis - Drug Effects - Genetics - Immunology - Microbiology | en_US |
dc.subject.mesh | Arabidopsis Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Arachidonic Acid - Pharmacology | en_US |
dc.subject.mesh | Botrytis - Drug Effects - Physiology | en_US |
dc.subject.mesh | Carrier Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Cell Death - Drug Effects | en_US |
dc.subject.mesh | Disease Resistance - Drug Effects - Immunology | en_US |
dc.subject.mesh | Gene Expression Regulation, Plant - Drug Effects | en_US |
dc.subject.mesh | Gene Knockout Techniques | en_US |
dc.subject.mesh | Genes, Plant - Genetics | en_US |
dc.subject.mesh | Green Fluorescent Proteins - Metabolism | en_US |
dc.subject.mesh | Hydrogen Peroxide - Metabolism | en_US |
dc.subject.mesh | Intracellular Space - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Oligonucleotide Array Sequence Analysis | en_US |
dc.subject.mesh | Phenotype | en_US |
dc.subject.mesh | Plant Diseases - Immunology - Microbiology | en_US |
dc.subject.mesh | Plant Growth Regulators - Pharmacology | en_US |
dc.subject.mesh | Pseudomonas Syringae - Drug Effects - Physiology | en_US |
dc.subject.mesh | Rna, Messenger - Genetics - Metabolism | en_US |
dc.subject.mesh | Salicylic Acid - Metabolism | en_US |
dc.title | Overexpression of arabidopsis acbp3 enhances npr1-dependent plant resistance to pseudomonas syringe pv tomato dc3000 | en_US |
dc.type | Article | en_US |
dc.identifier.email | Xiao, S: xiaoshi@graduate.hku.hk | en_US |
dc.identifier.email | Chye, ML: mlchye@hkucc.hku.hk | en_US |
dc.identifier.authority | Xiao, S=rp00817 | en_US |
dc.identifier.authority | Chye, ML=rp00687 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1104/pp.111.176933 | en_US |
dc.identifier.pmid | 21670223 | - |
dc.identifier.pmcid | PMC3149925 | - |
dc.identifier.scopus | eid_2-s2.0-79961174645 | en_US |
dc.identifier.hkuros | 198562 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79961174645&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 156 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 2069 | en_US |
dc.identifier.epage | 2081 | en_US |
dc.identifier.eissn | 1532-2548 | - |
dc.identifier.isi | WOS:000293568800032 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Xiao, S=7402022635 | en_US |
dc.identifier.scopusauthorid | Chye, ML=7003905460 | en_US |
dc.identifier.issnl | 0032-0889 | - |