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- Publisher Website: 10.1111/j.1750-3841.2011.02422.x
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- PMID: 22416710
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Article: Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature
Title | Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature |
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Authors | |
Keywords | Alginate-based microencapsulation Bifidobacteria Long-term storage Low a w Probiotic |
Issue Date | 2011 |
Publisher | Wiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147 |
Citation | Journal of Food Science, 2011, v. 76 n. 9, p. M592-M599 How to Cite? |
Abstract | Survival, acid and bile tolerance, and surface hydrophobicity of microencapsulated Bifidobacterium animalis ssp. lactis Bb12 were studied during storage at room temperature (25 °C) at low water activity (0.07, 0.1, and 0.2). Two types of alginate-based systems were prepared with and without mannitol as microencapsulant of B. animalis ssp. lactis Bb12. Formation of gel beads containing cells was achieved by dropping each emulsion into CaCl 2 solution; then, the beads were freeze dried. Survival, acid tolerance during 2-h exposure in de Man, Rogosa, Sharpe (MRS) broth at pH 2.0, bile tolerance during 8-h exposure in MRS broth containing taurocholic acid at pH 5.8, and retention of surface hydrophobicity were determined after freeze drying and during storage. The result showed that neither alginate nor alginate-mannitol formulation was effective in protecting B. animalis ssp. lactis Bb12 during freezing and freeze drying. The viability in alginate-mannitol and alginate formulations after freeze drying was 6.61 and 6.34 log CFU/g, respectively. Storage at low a w improved survival, acid tolerance, bile tolerance, and surface hydrophobicity retention of microencapsulated B. animalis ssp. lactis Bb12 when compared with controlled storage in an aluminum foil (with a w of 0.38 and 0.40 for alginate-mannitol and alginate formulations, respectively). Alginate mannitol was more effective than the alginate system during a short period of storage, but its effectiveness decreased during a long period of storage (80% survival at 10 wk). Nevertheless, storage of microencapsulated B. animalis ssp. lactis Bb12 in an aluminum foil without a w adjustment during 10 wk at room temperature was not effective (survival was 64% to 65%). © 2011 Institute of Food Technologists ®. |
Persistent Identifier | http://hdl.handle.net/10722/179247 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.783 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dianawati, D | en_US |
dc.contributor.author | Shah, NP | en_US |
dc.date.accessioned | 2012-12-19T09:53:22Z | - |
dc.date.available | 2012-12-19T09:53:22Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Journal of Food Science, 2011, v. 76 n. 9, p. M592-M599 | en_US |
dc.identifier.issn | 0022-1147 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179247 | - |
dc.description.abstract | Survival, acid and bile tolerance, and surface hydrophobicity of microencapsulated Bifidobacterium animalis ssp. lactis Bb12 were studied during storage at room temperature (25 °C) at low water activity (0.07, 0.1, and 0.2). Two types of alginate-based systems were prepared with and without mannitol as microencapsulant of B. animalis ssp. lactis Bb12. Formation of gel beads containing cells was achieved by dropping each emulsion into CaCl 2 solution; then, the beads were freeze dried. Survival, acid tolerance during 2-h exposure in de Man, Rogosa, Sharpe (MRS) broth at pH 2.0, bile tolerance during 8-h exposure in MRS broth containing taurocholic acid at pH 5.8, and retention of surface hydrophobicity were determined after freeze drying and during storage. The result showed that neither alginate nor alginate-mannitol formulation was effective in protecting B. animalis ssp. lactis Bb12 during freezing and freeze drying. The viability in alginate-mannitol and alginate formulations after freeze drying was 6.61 and 6.34 log CFU/g, respectively. Storage at low a w improved survival, acid tolerance, bile tolerance, and surface hydrophobicity retention of microencapsulated B. animalis ssp. lactis Bb12 when compared with controlled storage in an aluminum foil (with a w of 0.38 and 0.40 for alginate-mannitol and alginate formulations, respectively). Alginate mannitol was more effective than the alginate system during a short period of storage, but its effectiveness decreased during a long period of storage (80% survival at 10 wk). Nevertheless, storage of microencapsulated B. animalis ssp. lactis Bb12 in an aluminum foil without a w adjustment during 10 wk at room temperature was not effective (survival was 64% to 65%). © 2011 Institute of Food Technologists ®. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147 | en_US |
dc.relation.ispartof | Journal of Food Science | en_US |
dc.subject | Alginate-based microencapsulation | - |
dc.subject | Bifidobacteria | - |
dc.subject | Long-term storage | - |
dc.subject | Low a w | - |
dc.subject | Probiotic | - |
dc.subject.mesh | Alginates - Metabolism | en_US |
dc.subject.mesh | Bifidobacterium - Growth & Development - Isolation & Purification | en_US |
dc.subject.mesh | Bile - Microbiology | en_US |
dc.subject.mesh | Colony Count, Microbial | en_US |
dc.subject.mesh | Drug Compounding | en_US |
dc.subject.mesh | Food Microbiology | en_US |
dc.subject.mesh | Food Preservation - Methods | en_US |
dc.subject.mesh | Food Storage - Methods | en_US |
dc.subject.mesh | Freeze Drying | en_US |
dc.subject.mesh | Gastric Acid - Metabolism | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Hydrophobic And Hydrophilic Interactions | en_US |
dc.subject.mesh | Mannitol - Metabolism | en_US |
dc.subject.mesh | Microbial Viability | en_US |
dc.subject.mesh | Probiotics | en_US |
dc.subject.mesh | Temperature | en_US |
dc.title | Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shah, NP: npshah@hku.hk | en_US |
dc.identifier.authority | Shah, NP=rp01571 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1750-3841.2011.02422.x | en_US |
dc.identifier.pmid | 22416710 | - |
dc.identifier.scopus | eid_2-s2.0-79961128260 | en_US |
dc.identifier.hkuros | 205695 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79961128260&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 76 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.spage | M592 | en_US |
dc.identifier.epage | M599 | en_US |
dc.identifier.isi | WOS:000297457000027 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Dianawati, D=40761270000 | en_US |
dc.identifier.scopusauthorid | Shah, NP=7401823907 | en_US |
dc.identifier.issnl | 0022-1147 | - |