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- Publisher Website: 10.1111/j.1750-3841.2011.02246.x
- Scopus: eid_2-s2.0-79961128183
- PMID: 21696390
- WOS: WOS:000293633100062
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Article: Enzyme Stability of Microencapsulated Bifidobacterium animalis ssp. lactis Bb12 after Freeze Drying and during Storage in Low Water Activity at Room Temperature
Title | Enzyme Stability of Microencapsulated Bifidobacterium animalis ssp. lactis Bb12 after Freeze Drying and during Storage in Low Water Activity at Room Temperature |
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Authors | |
Keywords | Long-term storage Low a w Mannitol Sodium alginate-based system Stability of enzymes activities |
Issue Date | 2011 |
Publisher | Wiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147 |
Citation | Journal Of Food Science, 2011, v. 76 n. 6, p. M463-M471 How to Cite? |
Abstract | Stability of enzymes such as β-galactosidase (β-gal), β-glucosidase (β-glu), lactate dehydrogenase (LDH), pyruvate kinase (PK), hexokinase (HK), and ATPase of microencapsulatedBifidobacterium animalisssp.lactisBb12 after freeze-drying and after 10 wk of storage at low water activity (a w) at room temperature was studied. Bacteria were microencapsulated using alginate formulation with or without mannitol fortification (sodium alginate and mannitol [SAM] and sodium alginate [SA], respectively) by creating gel beads followed by freeze drying. Two types of dried gel beads were then stored at low a w, such as 0.07, 0.1, and 0.2; storage in an aluminum foil was used as control. All storage was carried out at room temperature of 25 °C for 10 wk. Measurement of β-gal, β-glu, LDH, PK, HK, and ATPase (with or without exposure to pH 2.0 for 2 h) activities was carried out before freeze drying, after freeze drying, and after 10 wk of storage. There was a significant decrease in almost all enzyme activities, except that of PK. SAM and SA showed no different effect on maintaining enzyme activities during freeze drying. Storage for 10 wk at room temperature at various low a w using SAM and SA system had a significant effect on retention of most enzymes studied, except that of PK and LDH. Storage at a w of 0.07 and 0.1 was more effective in maintaining enzyme activities than storage at a w of 0.2 and in an aluminum foil. However, mannitol fortification into alginate system did not significantly improve retention of enzymes during 10 wk of storage. © 2011 Institute of Food Technologists ®. |
Persistent Identifier | http://hdl.handle.net/10722/179246 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.783 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Dianawati, D | en_US |
dc.contributor.author | Shah, NP | en_US |
dc.date.accessioned | 2012-12-19T09:53:21Z | - |
dc.date.available | 2012-12-19T09:53:21Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Journal Of Food Science, 2011, v. 76 n. 6, p. M463-M471 | en_US |
dc.identifier.issn | 0022-1147 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179246 | - |
dc.description.abstract | Stability of enzymes such as β-galactosidase (β-gal), β-glucosidase (β-glu), lactate dehydrogenase (LDH), pyruvate kinase (PK), hexokinase (HK), and ATPase of microencapsulatedBifidobacterium animalisssp.lactisBb12 after freeze-drying and after 10 wk of storage at low water activity (a w) at room temperature was studied. Bacteria were microencapsulated using alginate formulation with or without mannitol fortification (sodium alginate and mannitol [SAM] and sodium alginate [SA], respectively) by creating gel beads followed by freeze drying. Two types of dried gel beads were then stored at low a w, such as 0.07, 0.1, and 0.2; storage in an aluminum foil was used as control. All storage was carried out at room temperature of 25 °C for 10 wk. Measurement of β-gal, β-glu, LDH, PK, HK, and ATPase (with or without exposure to pH 2.0 for 2 h) activities was carried out before freeze drying, after freeze drying, and after 10 wk of storage. There was a significant decrease in almost all enzyme activities, except that of PK. SAM and SA showed no different effect on maintaining enzyme activities during freeze drying. Storage for 10 wk at room temperature at various low a w using SAM and SA system had a significant effect on retention of most enzymes studied, except that of PK and LDH. Storage at a w of 0.07 and 0.1 was more effective in maintaining enzyme activities than storage at a w of 0.2 and in an aluminum foil. However, mannitol fortification into alginate system did not significantly improve retention of enzymes during 10 wk of storage. © 2011 Institute of Food Technologists ®. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147 | en_US |
dc.relation.ispartof | Journal of Food Science | en_US |
dc.rights | The definitive version is available at www3.interscience.wiley.com | - |
dc.subject | Long-term storage | - |
dc.subject | Low a w | - |
dc.subject | Mannitol | - |
dc.subject | Sodium alginate-based system | - |
dc.subject | Stability of enzymes activities | - |
dc.subject.mesh | Adenosine Triphosphatases - Chemistry - Metabolism | en_US |
dc.subject.mesh | Alginates - Chemistry | en_US |
dc.subject.mesh | Bacterial Proteins - Chemistry - Metabolism | en_US |
dc.subject.mesh | Bifidobacterium - Enzymology | en_US |
dc.subject.mesh | Emulsions | en_US |
dc.subject.mesh | Enzyme Stability | en_US |
dc.subject.mesh | Food Technology | en_US |
dc.subject.mesh | Freeze Drying | en_US |
dc.subject.mesh | Gels | en_US |
dc.subject.mesh | Glucuronic Acid - Chemistry | en_US |
dc.subject.mesh | Glycoside Hydrolases - Chemistry - Metabolism | en_US |
dc.subject.mesh | Hexuronic Acids - Chemistry | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Mannitol - Chemistry | en_US |
dc.subject.mesh | Microspheres | en_US |
dc.subject.mesh | Phosphotransferases (Alcohol Group Acceptor) - Chemistry - Metabolism | en_US |
dc.subject.mesh | Probiotics - Chemistry - Metabolism | en_US |
dc.subject.mesh | Temperature | en_US |
dc.subject.mesh | Time Factors | en_US |
dc.subject.mesh | Water - Analysis | en_US |
dc.title | Enzyme Stability of Microencapsulated Bifidobacterium animalis ssp. lactis Bb12 after Freeze Drying and during Storage in Low Water Activity at Room Temperature | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shah, NP: npshah@hku.hk | en_US |
dc.identifier.authority | Shah, NP=rp01571 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1750-3841.2011.02246.x | en_US |
dc.identifier.pmid | 21696390 | - |
dc.identifier.scopus | eid_2-s2.0-79961128183 | en_US |
dc.identifier.hkuros | 205694 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79961128183&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 76 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | M463 | en_US |
dc.identifier.epage | M471 | en_US |
dc.identifier.isi | WOS:000293633100062 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Dianawati, D=40761270000 | en_US |
dc.identifier.scopusauthorid | Shah, NP=7401823907 | en_US |
dc.identifier.issnl | 0022-1147 | - |