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Article: Overexpressing AtPAP15 enhances phosphorus efficiency in soybean
Title | Overexpressing AtPAP15 enhances phosphorus efficiency in soybean |
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Authors | |
Issue Date | 2009 |
Publisher | American Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org |
Citation | Plant Physiology, 2009, v. 151 n. 1, p. 233-240 How to Cite? |
Abstract | Low phosphorus (P) availability is a major constraint to crop growth and production, including soybean (Glycine max), on a global scale. However, 50% to 80% of the total P in agricultural soils exists as organic phosphate, which is unavailable to plants unless hydrolyzed to release inorganic phosphate. One strategy for improving crop P nutrition is the enhanced activity of acid phosphatases (APases) to obtain or remobilize inorganic phosphate from organic P sources. In this study, we overexpressed an Arabidopsis (Arabidopsis thaliana) purple APase gene (AtPAP15) containing a carrot (Daucus carota) extracellular targeting peptide in soybean hairy roots and found that the APase activity was increased by 1.5-fold in transgenic hairy roots. We subsequently transformed soybean plants with AtPAP15 and studied three homozygous overexpression lines of AtPAP15. The three transgenic lines exhibited significantly improved P efficiency with 117.8%, 56.5%, and 57.8% increases in plant dry weight, and 90.1%, 18.2%, and 62.6% increases in plant P content, respectively, as compared with wild-type plants grown on sand culture containing phytate as the sole P source. The transgenic soybean lines also exhibited a significant level of APase and phytase activity in leaves and root exudates, respectively. Furthermore, the transgenic lines exhibited improved yields when grown on acid soils, with 35.9%, 41.0%, and 59.0% increases in pod number per plant, and 46.0%, 48.3%, and 66.7% increases in seed number per plant. Taken together, to our knowledge, our study is the first report on the improvement of P efficiency in soybean through constitutive expression of a plant APase gene. These findings could have significant implications for improving crop yield on soils low in available P, which is a serious agricultural limitation worldwide. © 2009 American Society of Plant Biologists. |
Persistent Identifier | http://hdl.handle.net/10722/179158 |
ISSN | 2023 Impact Factor: 6.5 2023 SCImago Journal Rankings: 2.101 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Wang, X | en_US |
dc.contributor.author | Wang, Y | en_US |
dc.contributor.author | Tian, J | en_US |
dc.contributor.author | Lim, BL | en_US |
dc.contributor.author | Yan, X | en_US |
dc.contributor.author | Liao, H | en_US |
dc.date.accessioned | 2012-12-19T09:52:26Z | - |
dc.date.available | 2012-12-19T09:52:26Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Plant Physiology, 2009, v. 151 n. 1, p. 233-240 | en_US |
dc.identifier.issn | 0032-0889 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179158 | - |
dc.description.abstract | Low phosphorus (P) availability is a major constraint to crop growth and production, including soybean (Glycine max), on a global scale. However, 50% to 80% of the total P in agricultural soils exists as organic phosphate, which is unavailable to plants unless hydrolyzed to release inorganic phosphate. One strategy for improving crop P nutrition is the enhanced activity of acid phosphatases (APases) to obtain or remobilize inorganic phosphate from organic P sources. In this study, we overexpressed an Arabidopsis (Arabidopsis thaliana) purple APase gene (AtPAP15) containing a carrot (Daucus carota) extracellular targeting peptide in soybean hairy roots and found that the APase activity was increased by 1.5-fold in transgenic hairy roots. We subsequently transformed soybean plants with AtPAP15 and studied three homozygous overexpression lines of AtPAP15. The three transgenic lines exhibited significantly improved P efficiency with 117.8%, 56.5%, and 57.8% increases in plant dry weight, and 90.1%, 18.2%, and 62.6% increases in plant P content, respectively, as compared with wild-type plants grown on sand culture containing phytate as the sole P source. The transgenic soybean lines also exhibited a significant level of APase and phytase activity in leaves and root exudates, respectively. Furthermore, the transgenic lines exhibited improved yields when grown on acid soils, with 35.9%, 41.0%, and 59.0% increases in pod number per plant, and 46.0%, 48.3%, and 66.7% increases in seed number per plant. Taken together, to our knowledge, our study is the first report on the improvement of P efficiency in soybean through constitutive expression of a plant APase gene. These findings could have significant implications for improving crop yield on soils low in available P, which is a serious agricultural limitation worldwide. © 2009 American Society of Plant Biologists. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org | en_US |
dc.relation.ispartof | Plant Physiology | en_US |
dc.subject.mesh | 6-Phytase - Metabolism | en_US |
dc.subject.mesh | Acid Phosphatase - Genetics - Metabolism | en_US |
dc.subject.mesh | Arabidopsis Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Multienzyme Complexes - Genetics - Metabolism | en_US |
dc.subject.mesh | Phosphorus - Metabolism | en_US |
dc.subject.mesh | Plant Roots - Genetics - Metabolism | en_US |
dc.subject.mesh | Plants, Genetically Modified | en_US |
dc.subject.mesh | Soybeans - Enzymology - Genetics - Growth & Development - Metabolism | en_US |
dc.title | Overexpressing AtPAP15 enhances phosphorus efficiency in soybean | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lim, BL: bllim@hkucc.hku.hk | en_US |
dc.identifier.authority | Lim, BL=rp00744 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1104/pp.109.138891 | en_US |
dc.identifier.pmid | 19587103 | - |
dc.identifier.pmcid | PMC2736008 | - |
dc.identifier.scopus | eid_2-s2.0-70349213934 | en_US |
dc.identifier.hkuros | 164344 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-70349213934&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 151 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 233 | en_US |
dc.identifier.epage | 240 | en_US |
dc.identifier.isi | WOS:000269522200019 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wang, X=8695873600 | en_US |
dc.identifier.scopusauthorid | Wang, Y=7601494131 | en_US |
dc.identifier.scopusauthorid | Tian, J=55111185900 | en_US |
dc.identifier.scopusauthorid | Lim, BL=7201983917 | en_US |
dc.identifier.scopusauthorid | Yan, X=7403596595 | en_US |
dc.identifier.scopusauthorid | Liao, H=55121516700 | en_US |
dc.identifier.issnl | 0032-0889 | - |