File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1104/pp.109.143180
- Scopus: eid_2-s2.0-70349209373
- PMID: 19633233
- WOS: WOS:000269522200016
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Molecular and biochemical characterization of AtPAP15, a purple acid phosphatase with phytase activity, in Arabidopsis
Title | Molecular and biochemical characterization of AtPAP15, a purple acid phosphatase with phytase activity, in Arabidopsis |
---|---|
Authors | |
Issue Date | 2009 |
Publisher | American Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org |
Citation | Plant Physiology, 2009, v. 151 n. 1, p. 199-209 How to Cite? |
Abstract | Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with soybean (Glycine max; GmPHY) and tobacco (Nicotiana tabacum) PAP (NtPAP) with phytase activity than the other AtPAPs. In this study, transgenic Arabidopsis that expressed an AtPAP15 promoter:: b-glucuronidase (GUS) fusion protein showed that AtPAP15 expression was developmentally and temporally regulated, with strong GUS staining at the early stages of seedling growth and pollen germination. The expression was also organ/tissue specific, with strongest GUS staining in the vasculature, pollen grains, and roots. The recombinant AtPAP purified from transgenic tobacco exhibited broad substrate specificity with moderate phytase activity. AtPAP15 T-DNA insertion lines exhibited a lower phytase and phosphatase activity in seedling and germinating pollen and lower pollen germination rate compared with the wild type and their complementation lines. Therefore, AtPAP15 likely mobilizes phosphorus reserves in plants, particularly during seed and pollen germination. Since AtPAP15 is not expressed in the root hair or in the epidermal cells, it is unlikely to play any role in external phosphorus assimilation. © 2009 American Society of Plant Biologists. |
Persistent Identifier | http://hdl.handle.net/10722/179157 |
ISSN | 2023 Impact Factor: 6.5 2023 SCImago Journal Rankings: 2.101 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kuang, R | en_US |
dc.contributor.author | Chan, KH | en_US |
dc.contributor.author | Yeung, E | en_US |
dc.contributor.author | Lim, BL | en_US |
dc.date.accessioned | 2012-12-19T09:52:26Z | - |
dc.date.available | 2012-12-19T09:52:26Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Plant Physiology, 2009, v. 151 n. 1, p. 199-209 | en_US |
dc.identifier.issn | 0032-0889 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179157 | - |
dc.description.abstract | Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with soybean (Glycine max; GmPHY) and tobacco (Nicotiana tabacum) PAP (NtPAP) with phytase activity than the other AtPAPs. In this study, transgenic Arabidopsis that expressed an AtPAP15 promoter:: b-glucuronidase (GUS) fusion protein showed that AtPAP15 expression was developmentally and temporally regulated, with strong GUS staining at the early stages of seedling growth and pollen germination. The expression was also organ/tissue specific, with strongest GUS staining in the vasculature, pollen grains, and roots. The recombinant AtPAP purified from transgenic tobacco exhibited broad substrate specificity with moderate phytase activity. AtPAP15 T-DNA insertion lines exhibited a lower phytase and phosphatase activity in seedling and germinating pollen and lower pollen germination rate compared with the wild type and their complementation lines. Therefore, AtPAP15 likely mobilizes phosphorus reserves in plants, particularly during seed and pollen germination. Since AtPAP15 is not expressed in the root hair or in the epidermal cells, it is unlikely to play any role in external phosphorus assimilation. © 2009 American Society of Plant Biologists. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org | en_US |
dc.relation.ispartof | Plant Physiology | en_US |
dc.subject.mesh | Acid Phosphatase - Genetics - Metabolism | en_US |
dc.subject.mesh | Arabidopsis - Enzymology | en_US |
dc.subject.mesh | Arabidopsis Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Dna, Bacterial - Genetics | en_US |
dc.subject.mesh | Gene Expression Regulation, Enzymologic - Physiology | en_US |
dc.subject.mesh | Gene Expression Regulation, Plant - Physiology | en_US |
dc.subject.mesh | Genetic Complementation Test | en_US |
dc.subject.mesh | Multienzyme Complexes - Genetics - Metabolism | en_US |
dc.subject.mesh | Mutagenesis, Insertional | en_US |
dc.subject.mesh | Mutation | en_US |
dc.subject.mesh | Plants, Genetically Modified | en_US |
dc.subject.mesh | Substrate Specificity | en_US |
dc.subject.mesh | Tobacco - Genetics | en_US |
dc.title | Molecular and biochemical characterization of AtPAP15, a purple acid phosphatase with phytase activity, in Arabidopsis | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lim, BL: bllim@hkucc.hku.hk | en_US |
dc.identifier.authority | Lim, BL=rp00744 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1104/pp.109.143180 | en_US |
dc.identifier.pmid | 19633233 | - |
dc.identifier.pmcid | PMC2735976 | - |
dc.identifier.scopus | eid_2-s2.0-70349209373 | en_US |
dc.identifier.hkuros | 164336 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-70349209373&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 151 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 199 | en_US |
dc.identifier.epage | 209 | en_US |
dc.identifier.eissn | 1532-2548 | - |
dc.identifier.isi | WOS:000269522200016 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Kuang, R=41361483500 | en_US |
dc.identifier.scopusauthorid | Chan, KH=41361030400 | en_US |
dc.identifier.scopusauthorid | Yeung, E=7101971768 | en_US |
dc.identifier.scopusauthorid | Lim, BL=7201983917 | en_US |
dc.identifier.issnl | 0032-0889 | - |