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- Publisher Website: 10.1021/jf801404y
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- PMID: 18683947
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Article: Lipid characterization of Mortierella alpina grown at different NaCl concentrations
Title | Lipid characterization of Mortierella alpina grown at different NaCl concentrations |
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Authors | |
Keywords | Diacylglycerol acyltransferase 2 Mortierella alpina Polyunsaturated fatty acids Triacylglycerol |
Issue Date | 2008 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau |
Citation | Journal Of Agricultural And Food Chemistry, 2008, v. 56 n. 17, p. 7903-7909 How to Cite? |
Abstract | Effects of sodium chloride (NaCl) concentration on the lipid and fatty acid profiles of the polyunsaturated fatty acid (PUFA)-producing fungus, Mortierella alpina SC9, were investigated. The cells were cultivated in the medium with 4 different NaCl concentrations (0, 1, 2, 4%) for 6 days. The lipid and fatty acid profiles were analyzed by thin layer chromatography and gas chromatography. In the cultures with NaCl concentration up to 2%, PUFAs accounted for over 50% of the total fatty acids (TFAs) of the cells. Triacylglycerol (TAG) was the major lipid class, followed by monoacylglycerol (MAG) and diacylglycerol (DAG). TAG was found to contain the highest proportion of arachidonic acid (C20:4n-6, AA), suggesting that AA was mainly stored in the TAG. Comparing cultures at different NaCl concentrations indicated that TFA and TAG contents were higher in the cells grown at 2% NaCl. Similar results were found when 2% NaCl was added at day 3 of cultivation (late-log phase). In addition, the gene expression level of a TAG biosynthesis enzyme, diacylglycerol acyltransferase 2 (DGAT2), was also higher in the NaCl treated cells. This suggested that the increase of TFA and TAG contents might be related to the NaCl-stimulated DGAT2 expression. © 2008 American Chemical Society. |
Persistent Identifier | http://hdl.handle.net/10722/179086 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.114 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Ho, SY | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:51:51Z | - |
dc.date.available | 2012-12-19T09:51:51Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Journal Of Agricultural And Food Chemistry, 2008, v. 56 n. 17, p. 7903-7909 | en_US |
dc.identifier.issn | 0021-8561 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179086 | - |
dc.description.abstract | Effects of sodium chloride (NaCl) concentration on the lipid and fatty acid profiles of the polyunsaturated fatty acid (PUFA)-producing fungus, Mortierella alpina SC9, were investigated. The cells were cultivated in the medium with 4 different NaCl concentrations (0, 1, 2, 4%) for 6 days. The lipid and fatty acid profiles were analyzed by thin layer chromatography and gas chromatography. In the cultures with NaCl concentration up to 2%, PUFAs accounted for over 50% of the total fatty acids (TFAs) of the cells. Triacylglycerol (TAG) was the major lipid class, followed by monoacylglycerol (MAG) and diacylglycerol (DAG). TAG was found to contain the highest proportion of arachidonic acid (C20:4n-6, AA), suggesting that AA was mainly stored in the TAG. Comparing cultures at different NaCl concentrations indicated that TFA and TAG contents were higher in the cells grown at 2% NaCl. Similar results were found when 2% NaCl was added at day 3 of cultivation (late-log phase). In addition, the gene expression level of a TAG biosynthesis enzyme, diacylglycerol acyltransferase 2 (DGAT2), was also higher in the NaCl treated cells. This suggested that the increase of TFA and TAG contents might be related to the NaCl-stimulated DGAT2 expression. © 2008 American Chemical Society. | en_US |
dc.language | eng | en_US |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau | en_US |
dc.relation.ispartof | Journal of Agricultural and Food Chemistry | en_US |
dc.subject | Diacylglycerol acyltransferase 2 | - |
dc.subject | Mortierella alpina | - |
dc.subject | Polyunsaturated fatty acids | - |
dc.subject | Triacylglycerol | - |
dc.subject.mesh | Fatty Acids - Analysis | en_US |
dc.subject.mesh | Fatty Acids, Unsaturated - Analysis | en_US |
dc.subject.mesh | Lipids - Analysis | en_US |
dc.subject.mesh | Mortierella - Chemistry - Growth & Development | en_US |
dc.subject.mesh | Sodium Chloride - Administration & Dosage | en_US |
dc.subject.mesh | Triglycerides - Analysis | en_US |
dc.title | Lipid characterization of Mortierella alpina grown at different NaCl concentrations | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1021/jf801404y | en_US |
dc.identifier.pmid | 18683947 | - |
dc.identifier.scopus | eid_2-s2.0-52649144274 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-52649144274&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 56 | en_US |
dc.identifier.issue | 17 | en_US |
dc.identifier.spage | 7903 | en_US |
dc.identifier.epage | 7909 | en_US |
dc.identifier.isi | WOS:000258938000048 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Ho, SY=16426196000 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0021-8561 | - |