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- Publisher Website: 10.1093/ps/86.2.423
- Scopus: eid_2-s2.0-33947509354
- PMID: 17234861
- WOS: WOS:000243841800031
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Article: Cloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissues
Title | Cloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissues |
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Authors | |
Keywords | Chicken Embryo Glucocorticoid receptor Pituitary |
Issue Date | 2007 |
Publisher | Poultry Science Association Inc. The Journal's web site is located at http://ps.fass.org |
Citation | Poultry Science, 2007, v. 86 n. 2, p. 423-430 How to Cite? |
Abstract | Substantial evidence suggests that glucocorticoids play critical roles in the differentiation of somatotroph and lactotroph in embryonic pituitaries of birds. However, the basic information on the expression of glucocorticoid receptor (GR) in avian species is limited. In this study, the full-length cDNA for chicken GR was cloned from the chicken kidney. It encodes 772 amino acids and shares high homology with that of the human (73%), mouse (73%), rat (71%), rabbit (72%), and trout (51%) sequences. Similar to mammals, chicken GR is widely expressed in all adult tissues being investigated. Among the 12 tissues investigated, relatively high expression of GR was detected in pituitary, muscle, ovary, and kidney using reverse transcription-PCR assay. Using semiquantitative reverse transcription-PCR, GR is shown to be abundantly expressed at a more or less constant level during embryonic pituitary development (from d 8 to 20), supporting the hypothesis that the expression of GR is unlikely to be a limiting factor in initiating the differentiation of somatotroph and lactotroph in embryonic pituitary of birds. Moreover, an abundant expression of GR in the whole embryos at earlier developmental stages (from d 2 to 5) was also detected in the present study, though its physiological relevance remains to be determined. ©2007 Poultry Science Association Inc. |
Persistent Identifier | http://hdl.handle.net/10722/178986 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 1.080 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Kwok, AHY | en_US |
dc.contributor.author | Wang, Y | en_US |
dc.contributor.author | Wang, CY | en_US |
dc.contributor.author | Leung, FC | en_US |
dc.date.accessioned | 2012-12-19T09:51:15Z | - |
dc.date.available | 2012-12-19T09:51:15Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Poultry Science, 2007, v. 86 n. 2, p. 423-430 | en_US |
dc.identifier.issn | 0032-5791 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178986 | - |
dc.description.abstract | Substantial evidence suggests that glucocorticoids play critical roles in the differentiation of somatotroph and lactotroph in embryonic pituitaries of birds. However, the basic information on the expression of glucocorticoid receptor (GR) in avian species is limited. In this study, the full-length cDNA for chicken GR was cloned from the chicken kidney. It encodes 772 amino acids and shares high homology with that of the human (73%), mouse (73%), rat (71%), rabbit (72%), and trout (51%) sequences. Similar to mammals, chicken GR is widely expressed in all adult tissues being investigated. Among the 12 tissues investigated, relatively high expression of GR was detected in pituitary, muscle, ovary, and kidney using reverse transcription-PCR assay. Using semiquantitative reverse transcription-PCR, GR is shown to be abundantly expressed at a more or less constant level during embryonic pituitary development (from d 8 to 20), supporting the hypothesis that the expression of GR is unlikely to be a limiting factor in initiating the differentiation of somatotroph and lactotroph in embryonic pituitary of birds. Moreover, an abundant expression of GR in the whole embryos at earlier developmental stages (from d 2 to 5) was also detected in the present study, though its physiological relevance remains to be determined. ©2007 Poultry Science Association Inc. | en_US |
dc.language | eng | en_US |
dc.publisher | Poultry Science Association Inc. The Journal's web site is located at http://ps.fass.org | en_US |
dc.relation.ispartof | Poultry Science | en_US |
dc.subject | Chicken | - |
dc.subject | Embryo | - |
dc.subject | Glucocorticoid receptor | - |
dc.subject | Pituitary | - |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Chick Embryo | en_US |
dc.subject.mesh | Chickens - Genetics | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gene Expression Profiling | en_US |
dc.subject.mesh | Gene Expression Regulation | en_US |
dc.subject.mesh | Kidney - Metabolism | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Muscle, Skeletal - Metabolism | en_US |
dc.subject.mesh | Ovary - Metabolism | en_US |
dc.subject.mesh | Phylogeny | en_US |
dc.subject.mesh | Pituitary Gland - Metabolism | en_US |
dc.subject.mesh | Rna, Messenger - Metabolism | en_US |
dc.subject.mesh | Receptors, Glucocorticoid - Chemistry - Genetics | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.title | Cloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissues | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wang, Y: cdwyj@yahoo.com | en_US |
dc.identifier.email | Leung, FC: fcleung@hkucc.hku.hk | en_US |
dc.identifier.authority | Wang, Y=rp00801 | en_US |
dc.identifier.authority | Leung, FC=rp00731 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1093/ps/86.2.423 | - |
dc.identifier.pmid | 17234861 | - |
dc.identifier.scopus | eid_2-s2.0-33947509354 | en_US |
dc.identifier.hkuros | 133446 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33947509354&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 86 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 423 | en_US |
dc.identifier.epage | 430 | en_US |
dc.identifier.isi | WOS:000243841800031 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Kwok, AHY=27168105100 | en_US |
dc.identifier.scopusauthorid | Wang, Y=36062525200 | en_US |
dc.identifier.scopusauthorid | Wang, CY=16065173000 | en_US |
dc.identifier.scopusauthorid | Leung, FC=7103078633 | en_US |
dc.identifier.issnl | 0032-5791 | - |