File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.2144/000112051
- Scopus: eid_2-s2.0-33645513978
- PMID: 16526408
- WOS: WOS:000235355300010
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Simultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCR
Title | Simultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCR |
---|---|
Authors | |
Issue Date | 2006 |
Publisher | Eaton Publishing Co. The Journal's web site is located at http://www.biotechniques.com |
Citation | Biotechniques, 2006, v. 40 n. 2, p. 187-189 How to Cite? |
Abstract | A new approach for simultaneously amplifying the 5′ and 3′ ends of a desired cDNA is described. The method combines the template-switching effect with inverse PCR, which generates the flanking 5′ and 3′ regions of a certain cDNA with very low or no background. It requires only minimal amounts of total RNA for the synthesis of first-strand cDNA, while the same cDNA can be used to amplify flanking sequences of any cDNA species present in the sample. This method is reliable and easy to perform, which is very useful for isolating cDNA species of rare transcripts. |
Persistent Identifier | http://hdl.handle.net/10722/178935 |
ISSN | 2023 Impact Factor: 2.2 2023 SCImago Journal Rankings: 0.557 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Huang, JC | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:50:51Z | - |
dc.date.available | 2012-12-19T09:50:51Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Biotechniques, 2006, v. 40 n. 2, p. 187-189 | en_US |
dc.identifier.issn | 0736-6205 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178935 | - |
dc.description.abstract | A new approach for simultaneously amplifying the 5′ and 3′ ends of a desired cDNA is described. The method combines the template-switching effect with inverse PCR, which generates the flanking 5′ and 3′ regions of a certain cDNA with very low or no background. It requires only minimal amounts of total RNA for the synthesis of first-strand cDNA, while the same cDNA can be used to amplify flanking sequences of any cDNA species present in the sample. This method is reliable and easy to perform, which is very useful for isolating cDNA species of rare transcripts. | en_US |
dc.language | eng | en_US |
dc.publisher | Eaton Publishing Co. The Journal's web site is located at http://www.biotechniques.com | en_US |
dc.relation.ispartof | BioTechniques | en_US |
dc.title | Simultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCR | en_US |
dc.type | Article | en_US |
dc.identifier.email | Huang, JC: huangjc@hku.hk | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Huang, JC=rp00710 | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.2144/000112051 | en_US |
dc.identifier.pmid | 16526408 | - |
dc.identifier.scopus | eid_2-s2.0-33645513978 | en_US |
dc.identifier.hkuros | 122423 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33645513978&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 40 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 187 | en_US |
dc.identifier.epage | 189 | en_US |
dc.identifier.isi | WOS:000235355300010 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Huang, JC=7408108735 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0736-6205 | - |