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Article: Isolation and proteomic alalysis of cell wall-deficient Haematococcus pluvialis mutants

TitleIsolation and proteomic alalysis of cell wall-deficient Haematococcus pluvialis mutants
Authors
KeywordsCell wall proteins
Cell wall-deficient mutant
Haematococcus pluvialis
Microalgae
PMF
Issue Date2005
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2005, v. 5 n. 18, p. 4839-4851 How to Cite?
AbstractThe green alga Haematococcus pluvialis has a plant-like cell wall consisting of glycoproteins and cellulose that is modified during the cell cycle and under various conditions. These features allow Haematococcus to be used as a model organism for studying cell wall biology. Development of the Haematococcus model is hampered by the absence of mutants that could provide insight into the biosynthesis and assembly of wall components. Haematococcus mutants (WM#537 and WM#2978) (WM-wall mutant) with defective cell walls were obtained by chemical mutagenesis. WM#537 features a secondary wall of considerably reduced thickness, whereas WM#2978 possesses a somewhat reduced secondary wall with little intervening space between the wall and plasmalemma. 2-DE revealed that a majority of the cell wall proteins were present in the wild-type and mutant cell walls throughout the cell cycle. PMF identified 55 wall protein orthologs from these strains, including a subset of induced proteins known to be involved in wall construction, remodeling, and defense. Down-regulation of certain wall proteins in the two mutants was associated with the wall defects, whereas overexpression of other proteins may have compensated for the defective walls in the two mutants. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/178919
ISSN
2023 Impact Factor: 3.4
2023 SCImago Journal Rankings: 1.011
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, SBen_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorSommerfeld, Men_US
dc.contributor.authorHu, Qen_US
dc.date.accessioned2012-12-19T09:50:44Z-
dc.date.available2012-12-19T09:50:44Z-
dc.date.issued2005en_US
dc.identifier.citationProteomics, 2005, v. 5 n. 18, p. 4839-4851en_US
dc.identifier.issn1615-9853en_US
dc.identifier.urihttp://hdl.handle.net/10722/178919-
dc.description.abstractThe green alga Haematococcus pluvialis has a plant-like cell wall consisting of glycoproteins and cellulose that is modified during the cell cycle and under various conditions. These features allow Haematococcus to be used as a model organism for studying cell wall biology. Development of the Haematococcus model is hampered by the absence of mutants that could provide insight into the biosynthesis and assembly of wall components. Haematococcus mutants (WM#537 and WM#2978) (WM-wall mutant) with defective cell walls were obtained by chemical mutagenesis. WM#537 features a secondary wall of considerably reduced thickness, whereas WM#2978 possesses a somewhat reduced secondary wall with little intervening space between the wall and plasmalemma. 2-DE revealed that a majority of the cell wall proteins were present in the wild-type and mutant cell walls throughout the cell cycle. PMF identified 55 wall protein orthologs from these strains, including a subset of induced proteins known to be involved in wall construction, remodeling, and defense. Down-regulation of certain wall proteins in the two mutants was associated with the wall defects, whereas overexpression of other proteins may have compensated for the defective walls in the two mutants. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_US
dc.relation.ispartofProteomicsen_US
dc.subjectCell wall proteins-
dc.subjectCell wall-deficient mutant-
dc.subjectHaematococcus pluvialis-
dc.subjectMicroalgae-
dc.subjectPMF-
dc.subject.meshAlgal Proteins - Biosynthesis - Chemistryen_US
dc.subject.meshCell Wall - Metabolism - Ultrastructureen_US
dc.subject.meshChlorophyta - Chemistry - Drug Effects - Genetics - Metabolism - Ultrastructureen_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshMethylnitronitrosoguanidine - Pharmacologyen_US
dc.subject.meshMutationen_US
dc.subject.meshProteome - Analysisen_US
dc.subject.meshUp-Regulationen_US
dc.titleIsolation and proteomic alalysis of cell wall-deficient Haematococcus pluvialis mutantsen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/pmic.200400092en_US
dc.identifier.pmid16281177-
dc.identifier.scopuseid_2-s2.0-29544452188en_US
dc.identifier.hkuros122427-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-29544452188&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume5en_US
dc.identifier.issue18en_US
dc.identifier.spage4839en_US
dc.identifier.epage4851en_US
dc.identifier.isiWOS:000234413400020-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridWang, SB=7410347419en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridSommerfeld, M=7007025132en_US
dc.identifier.scopusauthoridHu, Q=26666082400en_US
dc.identifier.issnl1615-9853-

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