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- Publisher Website: 10.1210/en.2002-0211
- Scopus: eid_2-s2.0-0037374822
- PMID: 12639893
- WOS: WOS:000182188900002
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Article: TGF-β3 regulates the blood-testis barrier dynamics via the p38 mitogen activated protein (MAP) kinase pathway: An in vivo study
Title | TGF-β3 regulates the blood-testis barrier dynamics via the p38 mitogen activated protein (MAP) kinase pathway: An in vivo study |
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Authors | |
Issue Date | 2003 |
Publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org |
Citation | Endocrinology, 2003, v. 144 n. 4, p. 1139-1142 How to Cite? |
Abstract | Recent studies using Sertoli cells cultured tn vitro to permit tight junction (TJ) assembly have shown that TJ dynamics are regulated, at least in part, by TGF-β3 via the p38 mitogen activated protein (MAP) kinase pathway. This in turn regulates the production of occludin, a TJ-integral membrane protein, by Sertoli cells. Yet it is not known if this pathway is used by Sertoli cells to regulate the blood-testis barrier (BTB) function in vivo. Using an in vivo model for studying BTB dynamics, we report herein the CdCl 2-induced BTB damage in rats was associated with a significant reduction in testieular oceludin along with a loss of immunoreaetive oeeludin in the seminiferous epithelium at the site of the BTB. Also, this CdCl 2-induced occludin loss from the BTB coincided with a surge in testicular TGF-β3, as well as p-p38 MAP kinase (the phosphorylated/activated form of p38), but not p38 MAP kinase and neither extracellular signal-regulated kinase nor its phosphorylated form (ERK/p-ERK), consistent with results of in vitro studies. More important, intratesticular administration of SB202190, a specific p38 MAP kinase inhibitor, could block the CdCl 2-induced occludin loss from the BTB. These results illustrate that BTB dynamics in vivo are regulated by the TGF-β3/p38 MAP kinase pathway, which in turn determines the level of occludin at the site of Sertoli cell TJs. |
Persistent Identifier | http://hdl.handle.net/10722/178790 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 1.285 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lui, WY | en_US |
dc.contributor.author | Wong, CH | en_US |
dc.contributor.author | Mruk, DD | en_US |
dc.contributor.author | Cheng, CY | en_US |
dc.date.accessioned | 2012-12-19T09:49:46Z | - |
dc.date.available | 2012-12-19T09:49:46Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Endocrinology, 2003, v. 144 n. 4, p. 1139-1142 | en_US |
dc.identifier.issn | 0013-7227 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178790 | - |
dc.description.abstract | Recent studies using Sertoli cells cultured tn vitro to permit tight junction (TJ) assembly have shown that TJ dynamics are regulated, at least in part, by TGF-β3 via the p38 mitogen activated protein (MAP) kinase pathway. This in turn regulates the production of occludin, a TJ-integral membrane protein, by Sertoli cells. Yet it is not known if this pathway is used by Sertoli cells to regulate the blood-testis barrier (BTB) function in vivo. Using an in vivo model for studying BTB dynamics, we report herein the CdCl 2-induced BTB damage in rats was associated with a significant reduction in testieular oceludin along with a loss of immunoreaetive oeeludin in the seminiferous epithelium at the site of the BTB. Also, this CdCl 2-induced occludin loss from the BTB coincided with a surge in testicular TGF-β3, as well as p-p38 MAP kinase (the phosphorylated/activated form of p38), but not p38 MAP kinase and neither extracellular signal-regulated kinase nor its phosphorylated form (ERK/p-ERK), consistent with results of in vitro studies. More important, intratesticular administration of SB202190, a specific p38 MAP kinase inhibitor, could block the CdCl 2-induced occludin loss from the BTB. These results illustrate that BTB dynamics in vivo are regulated by the TGF-β3/p38 MAP kinase pathway, which in turn determines the level of occludin at the site of Sertoli cell TJs. | en_US |
dc.language | eng | en_US |
dc.publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | en_US |
dc.relation.ispartof | Endocrinology | en_US |
dc.rights | Endocrinology . Copyright © The Endocrine Society. | - |
dc.title | TGF-β3 regulates the blood-testis barrier dynamics via the p38 mitogen activated protein (MAP) kinase pathway: An in vivo study | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lui, WY: wylui@hku.hk | en_US |
dc.identifier.authority | Lui, WY=rp00756 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1210/en.2002-0211 | en_US |
dc.identifier.pmid | 12639893 | - |
dc.identifier.scopus | eid_2-s2.0-0037374822 | en_US |
dc.identifier.hkuros | 100785 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037374822&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 144 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 1139 | en_US |
dc.identifier.epage | 1142 | en_US |
dc.identifier.isi | WOS:000182188900002 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lui, WY=35220192400 | en_US |
dc.identifier.scopusauthorid | Wong, CH=8849630400 | en_US |
dc.identifier.scopusauthorid | Mruk, DD=6701823934 | en_US |
dc.identifier.scopusauthorid | Cheng, CY=7404797787 | en_US |
dc.identifier.issnl | 0013-7227 | - |