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Article: Effects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitro

TitleEffects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitro
Authors
KeywordsCalcium
cAMP
Common carp
Pituitary adenylate cyclase activating polypeptide (PACAP)
Signal transduction
Issue Date2002
Citation
Acta Biochimica Et Biophysica Sinica, 2002, v. 34 n. 6, p. 790-795 How to Cite?
AbstractIn order to study signal transduction in PACAP-stimulated GH release, effects of ovine PACAPs (oPACAPs) on cAMP synthesis and intracellular free Ca 2+ ([Ca 2+ ] i) levels in primarily cultured common carp (Cyprinus carpio) pituitary cells were investigated using radioimmunoassay (RIA) for cAMP and [Ca 2+ ] i Indo1/AM loading techniques. The main results obtained could be briefly summarized as follows: parallel with their GH-releasing effects, oPACAP-38 (0.01 nmol/L to 1 μmol/L) and oPACAP-27 (0.01 nmol/L to 1 μmol/L) were also effective in increasing cAMP release, cellular cAMP content, and total cAMP production in a dose-dependent manner in common carp pituitary cells. In addition to their cAMP synthesis, 0.5 μmol/L oPACAP-38 and 0.5 μmol/L oPACAP-27 were also effective in elevating [Ca 2+ ] i levels in common carp pituitary cells preloaded with the Ca 2+ -sensitive dye Indo-1; the stimulatory actions were dependent on the presence of extracellular Ca 2+ ([Ca 2+ ] e), as removal of [Ca 2+ ] e by adding the Ca 2+ chelator 4 mmol/L EGTA blocked the rise of [Ca 2+ ] i induced by oPACAP-38 and oPACAP-27, respectively. Besides, the rise in [Ca 2+ ] i induced by 0.5 μmol/L oPACAP-38 was blocked by treatment with 10 μmol/L nifedipine, an inhibitor of "L-type" voltage-sensitive Ca 2+ channels (VSCC); but 0.5 μmol/L oPACAP-38 was not effective in elevating [Ca 2+ ] i levels after the cells treated with Ca 2+ -sensitive dye Indo-1 were challenged with 10 μmol/L nifedipine. These results, taken as a whole, strongly suggest the involvement of the cAMP- and Ca 2+ -dependent pathways in PACAP-stimulated GH release in common carp.
Persistent Identifierhttp://hdl.handle.net/10722/178778
ISSN
2003 Impact Factor: 0.524
References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Den_US
dc.contributor.authorChu, MSen_US
dc.contributor.authorLee, KYen_US
dc.contributor.authorWong, AOen_US
dc.contributor.authorLin, HRen_US
dc.date.accessioned2012-12-19T09:49:42Z-
dc.date.available2012-12-19T09:49:42Z-
dc.date.issued2002en_US
dc.identifier.citationActa Biochimica Et Biophysica Sinica, 2002, v. 34 n. 6, p. 790-795en_US
dc.identifier.issn0582-9879en_US
dc.identifier.urihttp://hdl.handle.net/10722/178778-
dc.description.abstractIn order to study signal transduction in PACAP-stimulated GH release, effects of ovine PACAPs (oPACAPs) on cAMP synthesis and intracellular free Ca 2+ ([Ca 2+ ] i) levels in primarily cultured common carp (Cyprinus carpio) pituitary cells were investigated using radioimmunoassay (RIA) for cAMP and [Ca 2+ ] i Indo1/AM loading techniques. The main results obtained could be briefly summarized as follows: parallel with their GH-releasing effects, oPACAP-38 (0.01 nmol/L to 1 μmol/L) and oPACAP-27 (0.01 nmol/L to 1 μmol/L) were also effective in increasing cAMP release, cellular cAMP content, and total cAMP production in a dose-dependent manner in common carp pituitary cells. In addition to their cAMP synthesis, 0.5 μmol/L oPACAP-38 and 0.5 μmol/L oPACAP-27 were also effective in elevating [Ca 2+ ] i levels in common carp pituitary cells preloaded with the Ca 2+ -sensitive dye Indo-1; the stimulatory actions were dependent on the presence of extracellular Ca 2+ ([Ca 2+ ] e), as removal of [Ca 2+ ] e by adding the Ca 2+ chelator 4 mmol/L EGTA blocked the rise of [Ca 2+ ] i induced by oPACAP-38 and oPACAP-27, respectively. Besides, the rise in [Ca 2+ ] i induced by 0.5 μmol/L oPACAP-38 was blocked by treatment with 10 μmol/L nifedipine, an inhibitor of "L-type" voltage-sensitive Ca 2+ channels (VSCC); but 0.5 μmol/L oPACAP-38 was not effective in elevating [Ca 2+ ] i levels after the cells treated with Ca 2+ -sensitive dye Indo-1 were challenged with 10 μmol/L nifedipine. These results, taken as a whole, strongly suggest the involvement of the cAMP- and Ca 2+ -dependent pathways in PACAP-stimulated GH release in common carp.en_US
dc.languageengen_US
dc.relation.ispartofActa Biochimica et Biophysica Sinicaen_US
dc.subjectCalcium-
dc.subjectcAMP-
dc.subjectCommon carp-
dc.subjectPituitary adenylate cyclase activating polypeptide (PACAP)-
dc.subjectSignal transduction-
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCalcium Channel Blockers - Pharmacologyen_US
dc.subject.meshCarpsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshNeuropeptides - Pharmacologyen_US
dc.subject.meshNifedipine - Pharmacologyen_US
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptideen_US
dc.subject.meshPituitary Gland - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshSheepen_US
dc.subject.meshTime Factorsen_US
dc.titleEffects of pituitary adenylate cyclase activating polypeptide (PACAP) on intracellular cAMP and Ca 2+ levels in common carp (Cyprinus carpio) pituitary cells in vitroen_US
dc.typeArticleen_US
dc.identifier.emailWong, AO: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AO=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12417926-
dc.identifier.scopuseid_2-s2.0-0036944502en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036944502&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume34en_US
dc.identifier.issue6en_US
dc.identifier.spage790en_US
dc.identifier.epage795en_US
dc.identifier.scopusauthoridXiao, D=45561966600en_US
dc.identifier.scopusauthoridChu, MS=7402457255en_US
dc.identifier.scopusauthoridLee, KY=26660356800en_US
dc.identifier.scopusauthoridWong, AO=7403147570en_US
dc.identifier.scopusauthoridLin, HR=7405571313en_US
dc.identifier.issnl0582-9879-

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