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- Publisher Website: 10.1016/S0021-9673(00)00987-0
- Scopus: eid_2-s2.0-0035808709
- PMID: 11206781
- WOS: WOS:000166339600014
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Article: Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography
Title | Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography |
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Authors | |
Keywords | Carotenoids Chlorella vulgaris Counter-current chromatography Lutein Preparative chromatography Vitamins |
Issue Date | 2001 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma |
Citation | Journal Of Chromatography A, 2001, v. 905 n. 1-2, p. 151-155 How to Cite? |
Abstract | High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation. Copyright (C) 2001 Elsevier Science B.V. |
Persistent Identifier | http://hdl.handle.net/10722/178756 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.717 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Chen, F | en_US |
dc.contributor.author | Zhang, TY | en_US |
dc.contributor.author | Yang, FQ | en_US |
dc.contributor.author | Xu, GQ | en_US |
dc.date.accessioned | 2012-12-19T09:49:32Z | - |
dc.date.available | 2012-12-19T09:49:32Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Journal Of Chromatography A, 2001, v. 905 n. 1-2, p. 151-155 | en_US |
dc.identifier.issn | 0021-9673 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178756 | - |
dc.description.abstract | High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation. Copyright (C) 2001 Elsevier Science B.V. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma | en_US |
dc.relation.ispartof | Journal of Chromatography A | en_US |
dc.rights | Journal of Chromatography: Biomedical Applications. Copyright © Elsevier BV. | - |
dc.subject | Carotenoids | - |
dc.subject | Chlorella vulgaris | - |
dc.subject | Counter-current chromatography | - |
dc.subject | Lutein | - |
dc.subject | Preparative chromatography | - |
dc.subject | Vitamins | - |
dc.subject.mesh | Chlorella - Chemistry | en_US |
dc.subject.mesh | Chromatography, High Pressure Liquid - Methods | en_US |
dc.subject.mesh | Lutein - Isolation & Purification | en_US |
dc.subject.mesh | Spectrophotometry, Ultraviolet | en_US |
dc.title | Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0021-9673(00)00987-0 | en_US |
dc.identifier.pmid | 11206781 | - |
dc.identifier.scopus | eid_2-s2.0-0035808709 | en_US |
dc.identifier.hkuros | 61487 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0035808709&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 905 | en_US |
dc.identifier.issue | 1-2 | en_US |
dc.identifier.spage | 151 | en_US |
dc.identifier.epage | 155 | en_US |
dc.identifier.isi | WOS:000166339600014 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.scopusauthorid | Zhang, TY=7404374366 | en_US |
dc.identifier.scopusauthorid | Yang, FQ=7403449964 | en_US |
dc.identifier.scopusauthorid | Xu, GQ=14030714200 | en_US |
dc.identifier.issnl | 0021-9673 | - |