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- Publisher Website: 10.1016/S0021-9673(00)00724-X
- Scopus: eid_2-s2.0-0034622676
- PMID: 11043784
- WOS: WOS:000089286000004
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Article: Determination of vitamin B12 in multivitamin tablets and fermentation medium by high-performance liquid chromatography with fluorescence detection
Title | Determination of vitamin B12 in multivitamin tablets and fermentation medium by high-performance liquid chromatography with fluorescence detection |
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Authors | |
Keywords | Pharmaceutical analysis Vitamins |
Issue Date | 2000 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma |
Citation | Journal Of Chromatography A, 2000, v. 891 n. 2, p. 243-247 How to Cite? |
Abstract | A novel method for the determination of vitamin B12 by high- performance liquid chromatography with fluorescence detection is reported. The method was simple and highly sensitive with good precision. Vitamin B12 was analyzed by HPLC on a μBondapak C18 column (300 x 3.9 mm, 10 μm) with methanol-water (30:70) as mobile phase and fluorescence detection at 305 nm (with excitation at 275 nm). The calibration graph was linear from 1.000 to 100.0 ng ml-1 for vitamin B12 with a correlation coefficient of 0.998 (n = 6). The detection limit was 0.1 ng ml-1. The method was successfully applied to the determination of vitamin B12 in vitamin B12 tablets, multivitamin tablets and fermentation medium. The recovery was from 94 to 102% and the relative standard deviation was in the range of 1.8 to 4.1%. (C) 2000 Elsevier Science B.V. |
Persistent Identifier | http://hdl.handle.net/10722/178722 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.717 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Chen, F | en_US |
dc.contributor.author | Jiang, Y | en_US |
dc.date.accessioned | 2012-12-19T09:49:20Z | - |
dc.date.available | 2012-12-19T09:49:20Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | Journal Of Chromatography A, 2000, v. 891 n. 2, p. 243-247 | en_US |
dc.identifier.issn | 0021-9673 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178722 | - |
dc.description.abstract | A novel method for the determination of vitamin B12 by high- performance liquid chromatography with fluorescence detection is reported. The method was simple and highly sensitive with good precision. Vitamin B12 was analyzed by HPLC on a μBondapak C18 column (300 x 3.9 mm, 10 μm) with methanol-water (30:70) as mobile phase and fluorescence detection at 305 nm (with excitation at 275 nm). The calibration graph was linear from 1.000 to 100.0 ng ml-1 for vitamin B12 with a correlation coefficient of 0.998 (n = 6). The detection limit was 0.1 ng ml-1. The method was successfully applied to the determination of vitamin B12 in vitamin B12 tablets, multivitamin tablets and fermentation medium. The recovery was from 94 to 102% and the relative standard deviation was in the range of 1.8 to 4.1%. (C) 2000 Elsevier Science B.V. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma | en_US |
dc.relation.ispartof | Journal of Chromatography A | en_US |
dc.subject | Pharmaceutical analysis | - |
dc.subject | Vitamins | - |
dc.subject.mesh | Chromatography, High Pressure Liquid - Methods | en_US |
dc.subject.mesh | Culture Media - Chemistry | en_US |
dc.subject.mesh | Fermentation | en_US |
dc.subject.mesh | Sensitivity And Specificity | en_US |
dc.subject.mesh | Spectrometry, Fluorescence | en_US |
dc.subject.mesh | Tablets - Chemistry | en_US |
dc.subject.mesh | Vitamin B 12 - Analysis | en_US |
dc.subject.mesh | Vitamins - Chemistry | en_US |
dc.title | Determination of vitamin B12 in multivitamin tablets and fermentation medium by high-performance liquid chromatography with fluorescence detection | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0021-9673(00)00724-X | en_US |
dc.identifier.pmid | 11043784 | - |
dc.identifier.scopus | eid_2-s2.0-0034622676 | en_US |
dc.identifier.hkuros | 61526 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034622676&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 891 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 243 | en_US |
dc.identifier.epage | 247 | en_US |
dc.identifier.isi | WOS:000089286000004 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Li, HB=36066773900 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.scopusauthorid | Jiang, Y=24605346600 | en_US |
dc.identifier.issnl | 0021-9673 | - |