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- Publisher Website: 10.1007/s002160000478
- Scopus: eid_2-s2.0-0034330348
- PMID: 11227533
- WOS: WOS:000165143700014
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Article: A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al 3+-calcein complex
Title | A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al 3+-calcein complex |
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Authors | |
Issue Date | 2000 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm |
Citation | Fresenius' Journal Of Analytical Chemistry, 2000, v. 368 n. 5, p. 501-504 How to Cite? |
Abstract | A highly sensitive fluorimetric method for the determination of fluoride was established. The method was based on quenching of the fluorescence of the Al 3+-calcein complex in CCl 3COOH-CH 3COOK buffer solution by fluoride. The fluorescence emission was measured at λ ex/λ em 480/503 nm, and the experimental variables and interference in this determination were studied. The linear calibration range was 0.8 ng/mL to 150 ng/mL and the detection limit was 0.2 ng/mL. The method was applied to determine fluoride in biological materials. The recovery was in the range of 96.3% to 104.7% and the relative standard deviation was 4.6%. The results obtained from the certified reference material by the present method agreed with the certified values. © Springer-Verlag 2000. |
Persistent Identifier | http://hdl.handle.net/10722/178714 |
ISSN | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:49:17Z | - |
dc.date.available | 2012-12-19T09:49:17Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | Fresenius' Journal Of Analytical Chemistry, 2000, v. 368 n. 5, p. 501-504 | en_US |
dc.identifier.issn | 0937-0633 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178714 | - |
dc.description.abstract | A highly sensitive fluorimetric method for the determination of fluoride was established. The method was based on quenching of the fluorescence of the Al 3+-calcein complex in CCl 3COOH-CH 3COOK buffer solution by fluoride. The fluorescence emission was measured at λ ex/λ em 480/503 nm, and the experimental variables and interference in this determination were studied. The linear calibration range was 0.8 ng/mL to 150 ng/mL and the detection limit was 0.2 ng/mL. The method was applied to determine fluoride in biological materials. The recovery was in the range of 96.3% to 104.7% and the relative standard deviation was 4.6%. The results obtained from the certified reference material by the present method agreed with the certified values. © Springer-Verlag 2000. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm | en_US |
dc.relation.ispartof | Fresenius' Journal of Analytical Chemistry | en_US |
dc.title | A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al 3+-calcein complex | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/s002160000478 | - |
dc.identifier.pmid | 11227533 | - |
dc.identifier.scopus | eid_2-s2.0-0034330348 | en_US |
dc.identifier.hkuros | 61520 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034330348&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 368 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 501 | en_US |
dc.identifier.epage | 504 | en_US |
dc.identifier.isi | WOS:000165143700014 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Li, HB=36066773900 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0937-0633 | - |