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- Scopus: eid_2-s2.0-0025911581
- PMID: 2008439
- WOS: WOS:A1991FE55900004
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Article: Elevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs
Title | Elevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs |
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Authors | |
Issue Date | 1991 |
Publisher | Society for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/ |
Citation | Proceedings Of The Society For Experimental Biology And Medicine, 1991, v. 196 n. 4, p. 385-389 How to Cite? |
Abstract | The objective of this study is to examine and characterize epidermal growth factor receptor (EGF-R) binding in inhaled plutonium-induced canine lung-tumor tissue and to compare it with that in normal canine lung tissue. Crude membrane preparations from normal and lung-tumor tissue from beagle dogs were examined in a radioreceptor assay, using 125I-labeled epidermal growth factor (EGF) as a ligand. Specific EGF receptor binding was determined in the presence of excess unlabeled EGF. We have examined EGF receptor binding in eight lung-tumor samples obtained from six dogs. Epidermal growth factor receptor binding was significantly greater in lung-tumor samples (31.38%) compared with that in normal lung tissue (3.76%). Scatchard plot analysis from the displacement assay revealed that there was no statistical difference in the binding affinity but significantly higher concentration of EGF-R sites in the lung-tumor tissue (619 fmol/mg) than in normal lung tissue (53 fmol/mg). The increase in EGF-R number in plutonium-induced dog lung tumors does not seem to correlate with increase in the initial lung burden exposure to plutonium. Our results demonstrate that there is a significant increase in EGF-R binding in inhaled plutonium-induced dog lung tumors. |
Persistent Identifier | http://hdl.handle.net/10722/178507 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Leung, FC | en_US |
dc.contributor.author | Bohn, LR | en_US |
dc.contributor.author | Dagle, GE | en_US |
dc.date.accessioned | 2012-12-19T09:48:06Z | - |
dc.date.available | 2012-12-19T09:48:06Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Proceedings Of The Society For Experimental Biology And Medicine, 1991, v. 196 n. 4, p. 385-389 | en_US |
dc.identifier.issn | 0037-9727 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178507 | - |
dc.description.abstract | The objective of this study is to examine and characterize epidermal growth factor receptor (EGF-R) binding in inhaled plutonium-induced canine lung-tumor tissue and to compare it with that in normal canine lung tissue. Crude membrane preparations from normal and lung-tumor tissue from beagle dogs were examined in a radioreceptor assay, using 125I-labeled epidermal growth factor (EGF) as a ligand. Specific EGF receptor binding was determined in the presence of excess unlabeled EGF. We have examined EGF receptor binding in eight lung-tumor samples obtained from six dogs. Epidermal growth factor receptor binding was significantly greater in lung-tumor samples (31.38%) compared with that in normal lung tissue (3.76%). Scatchard plot analysis from the displacement assay revealed that there was no statistical difference in the binding affinity but significantly higher concentration of EGF-R sites in the lung-tumor tissue (619 fmol/mg) than in normal lung tissue (53 fmol/mg). The increase in EGF-R number in plutonium-induced dog lung tumors does not seem to correlate with increase in the initial lung burden exposure to plutonium. Our results demonstrate that there is a significant increase in EGF-R binding in inhaled plutonium-induced dog lung tumors. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/ | en_US |
dc.relation.ispartof | Proceedings of the Society for Experimental Biology and Medicine | en_US |
dc.subject.mesh | Adenocarcinoma, Papillary - Metabolism | en_US |
dc.subject.mesh | Administration, Inhalation | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Binding, Competitive | en_US |
dc.subject.mesh | Carcinoma, Bronchogenic - Metabolism | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Epidermal Growth Factor - Metabolism | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Lung Neoplasms - Etiology - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Proteins - Metabolism | en_US |
dc.subject.mesh | Neoplasms, Radiation-Induced | en_US |
dc.subject.mesh | Plutonium | en_US |
dc.subject.mesh | Radioligand Assay | en_US |
dc.subject.mesh | Receptor, Epidermal Growth Factor - Metabolism | en_US |
dc.title | Elevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs | en_US |
dc.type | Article | en_US |
dc.identifier.email | Leung, FC: fcleung@hkucc.hku.hk | en_US |
dc.identifier.authority | Leung, FC=rp00731 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 2008439 | - |
dc.identifier.scopus | eid_2-s2.0-0025911581 | en_US |
dc.identifier.volume | 196 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 385 | en_US |
dc.identifier.epage | 389 | en_US |
dc.identifier.isi | WOS:A1991FE55900004 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Leung, FC=7103078633 | en_US |
dc.identifier.scopusauthorid | Bohn, LR=7003466457 | en_US |
dc.identifier.scopusauthorid | Dagle, GE=7004357391 | en_US |
dc.identifier.issnl | 0037-9727 | - |