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Article: A comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter

TitleA comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter
Authors
MartinGallardo, AMontoyaZavala, MKelder, BTaylor, JChen, HLeung, FCKopchick, JJ
Keywordsanimal cell expression assay
enhancers
eukaryotic viral promoters
plasmids
Recombinant DNA
transient expression
Issue Date1988
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 1988, v. 70 n. 1, p. 51-56 How to Cite?
DOI: http://dx.doi.org/10.1016/0378-1119(88)90103-5
AbstractThree transcriptional regulatory regions including a human cytomegalovirus immediate-early region (CMVIE) promoter, the simian virus-40 early region (SV40E) promoter, and Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) were ligated to the bovine growth hormone (bGH)-coding gene. Using a bGH transient expression system, the CMVIE and SV40E gene transcriptional regulatory regions were found to be approximately two-fold more efficient than the MoMLV LTR in directing expression of bGH in mouse L cells.
Persistent Identifierhttp://hdl.handle.net/10722/178463
ISSN
0378-1119
2023 Impact Factor: 2.6
2023 SCImago Journal Rankings: 0.725
ISI Accession Number ID
WOS:A1988Q891600005

 

DC FieldValueLanguage
dc.contributor.authorMartinGallardo, Aen_US
dc.contributor.authorMontoyaZavala, Men_US
dc.contributor.authorKelder, Ben_US
dc.contributor.authorTaylor, Jen_US
dc.contributor.authorChen, Hen_US
dc.contributor.authorLeung, FCen_US
dc.contributor.authorKopchick, JJen_US
dc.date.accessioned2012-12-19T09:47:50Z-
dc.date.available2012-12-19T09:47:50Z-
dc.date.issued1988en_US
dc.identifier.citationGene, 1988, v. 70 n. 1, p. 51-56en_US
dc.identifier.issn0378-1119en_US
dc.identifier.urihttp://hdl.handle.net/10722/178463-
dc.description.abstractThree transcriptional regulatory regions including a human cytomegalovirus immediate-early region (CMVIE) promoter, the simian virus-40 early region (SV40E) promoter, and Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) were ligated to the bovine growth hormone (bGH)-coding gene. Using a bGH transient expression system, the CMVIE and SV40E gene transcriptional regulatory regions were found to be approximately two-fold more efficient than the MoMLV LTR in directing expression of bGH in mouse L cells.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_US
dc.relation.ispartofGeneen_US
dc.subjectanimal cell expression assay-
dc.subjectenhancers-
dc.subjecteukaryotic viral promoters-
dc.subjectplasmids-
dc.subjectRecombinant DNA-
dc.subjecttransient expression-
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshCytomegalovirus - Geneticsen_US
dc.subject.meshDna, Recombinanten_US
dc.subject.meshGenes, Regulatoren_US
dc.subject.meshGenetic Vectorsen_US
dc.subject.meshGrowth Hormone - Geneticsen_US
dc.subject.meshL Cells (Cell Line)en_US
dc.subject.meshMiceen_US
dc.subject.meshMoloney Murine Leukemia Virus - Geneticsen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshSimian Virus 40 - Geneticsen_US
dc.subject.meshTransfectionen_US
dc.titleA comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoteren_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0378-1119(88)90103-5-
dc.identifier.pmid2853691-
dc.identifier.scopuseid_2-s2.0-0023691796en_US
dc.identifier.volume70en_US
dc.identifier.issue1en_US
dc.identifier.spage51en_US
dc.identifier.epage56en_US
dc.identifier.isiWOS:A1988Q891600005-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridMartinGallardo, A=6603831056en_US
dc.identifier.scopusauthoridMontoyaZavala, M=6506531096en_US
dc.identifier.scopusauthoridKelder, B=6701847446en_US
dc.identifier.scopusauthoridTaylor, J=7405405625en_US
dc.identifier.scopusauthoridChen, H=7501612240en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US
dc.identifier.scopusauthoridKopchick, JJ=7005438073en_US
dc.identifier.issnl0378-1119-

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