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- Publisher Website: 10.1111/j.1365-2567.2007.02580.x
- Scopus: eid_2-s2.0-34249327710
- PMID: 17376198
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Article: A role for mitogen-activated protein kinase and Ets-1 in the induction of interleukin-10 transcription by human immunodeficiency virus-1 Tat
Title | A role for mitogen-activated protein kinase and Ets-1 in the induction of interleukin-10 transcription by human immunodeficiency virus-1 Tat |
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Authors | |
Keywords | HIV Tat protein HIV-1 Interleukin-10 Transcription factors |
Issue Date | 2007 |
Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMM |
Citation | Immunology, 2007, v. 121 n. 3, p. 337-348 How to Cite? |
Abstract | The human immunodeficiency virus (HIV) Tat protein has multiple regulatory roles, including trans-activation of the HIV genome and regulation of immune signalling processes, including kinase activation and cytokine expression. We recently demonstrated that HIV-1 Tat induces the expression of interleukin (IL)-10 via p38 mitogen-activated protein kinase (MAPK) activation. We further delineated that the Tat-responsive element of the IL-10 promoter was located within 625 to 595 bp upstream from the transcription start site. Using electrophoretic mobility shift assays, the transcription factors Ets-1 and Sp-1 were shown to bind to the IL-10 promoter to activate transcription of the gene. Furthermore, sequential deletional mutations of the Ets-1- and Sp-1-binding sites in the -625/-595 region reduced the DNA binding and transcription activity of the IL-10 promoter. Our results also showed that both the Tat-induced and Ets-1-regulated IL-10 promoter-driven luciferase activity can be abrogated by inhibitors of the p38 MAPK activity. In conclusion, the coordinated activities of p38 MAPK and the transcription factors, Ets-1 and Sp-1, may play an important role in the HIV-1 Tat-induced IL-10 transcription. © 2007 Blackwell Publishing Ltd. |
Persistent Identifier | http://hdl.handle.net/10722/175964 |
ISSN | 2023 Impact Factor: 4.9 2023 SCImago Journal Rankings: 1.720 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, JCB | en_US |
dc.contributor.author | Lau, ASY | en_US |
dc.date.accessioned | 2012-11-26T09:03:03Z | - |
dc.date.available | 2012-11-26T09:03:03Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Immunology, 2007, v. 121 n. 3, p. 337-348 | en_US |
dc.identifier.issn | 0019-2805 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/175964 | - |
dc.description.abstract | The human immunodeficiency virus (HIV) Tat protein has multiple regulatory roles, including trans-activation of the HIV genome and regulation of immune signalling processes, including kinase activation and cytokine expression. We recently demonstrated that HIV-1 Tat induces the expression of interleukin (IL)-10 via p38 mitogen-activated protein kinase (MAPK) activation. We further delineated that the Tat-responsive element of the IL-10 promoter was located within 625 to 595 bp upstream from the transcription start site. Using electrophoretic mobility shift assays, the transcription factors Ets-1 and Sp-1 were shown to bind to the IL-10 promoter to activate transcription of the gene. Furthermore, sequential deletional mutations of the Ets-1- and Sp-1-binding sites in the -625/-595 region reduced the DNA binding and transcription activity of the IL-10 promoter. Our results also showed that both the Tat-induced and Ets-1-regulated IL-10 promoter-driven luciferase activity can be abrogated by inhibitors of the p38 MAPK activity. In conclusion, the coordinated activities of p38 MAPK and the transcription factors, Ets-1 and Sp-1, may play an important role in the HIV-1 Tat-induced IL-10 transcription. © 2007 Blackwell Publishing Ltd. | en_US |
dc.language | eng | en_US |
dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMM | en_US |
dc.relation.ispartof | Immunology | en_US |
dc.rights | Immunology. Copyright © Blackwell Publishing Ltd. | - |
dc.subject | HIV Tat protein | - |
dc.subject | HIV-1 | - |
dc.subject | Interleukin-10 | - |
dc.subject | Transcription factors | - |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Binding Sites | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Dna-Binding Proteins - Metabolism | en_US |
dc.subject.mesh | Gene Products, Tat - Immunology | en_US |
dc.subject.mesh | Hiv-1 - Immunology | en_US |
dc.subject.mesh | Hela Cells | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Interleukin-10 - Genetics | en_US |
dc.subject.mesh | Promoter Regions, Genetic - Immunology | en_US |
dc.subject.mesh | Proto-Oncogene Protein C-Ets-1 - Genetics - Immunology | en_US |
dc.subject.mesh | Recombinant Proteins - Immunology | en_US |
dc.subject.mesh | Signal Transduction - Genetics - Immunology | en_US |
dc.subject.mesh | Transcription, Genetic - Immunology | en_US |
dc.subject.mesh | P38 Mitogen-Activated Protein Kinases - Immunology | en_US |
dc.subject.mesh | Tat Gene Products, Human Immunodeficiency Virus | en_US |
dc.title | A role for mitogen-activated protein kinase and Ets-1 in the induction of interleukin-10 transcription by human immunodeficiency virus-1 Tat | en_US |
dc.type | Article | en_US |
dc.identifier.email | Li, JCB: jamesli@hku.hk | en_US |
dc.identifier.email | Lau, ASY: asylau@hku.hk | en_US |
dc.identifier.authority | Li, JCB=rp00496 | en_US |
dc.identifier.authority | Lau, ASY=rp00474 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1365-2567.2007.02580.x | en_US |
dc.identifier.pmid | 17376198 | - |
dc.identifier.pmcid | PMC2265950 | - |
dc.identifier.scopus | eid_2-s2.0-34249327710 | en_US |
dc.identifier.hkuros | 126928 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-34249327710&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 121 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 337 | en_US |
dc.identifier.epage | 348 | en_US |
dc.identifier.eissn | 1365-2567 | - |
dc.identifier.isi | WOS:000246793800005 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Li, JCB=23103447500 | en_US |
dc.identifier.scopusauthorid | Lau, ASY=7202626202 | en_US |
dc.identifier.citeulike | 1341416 | - |
dc.identifier.issnl | 0019-2805 | - |