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- Publisher Website: 10.1093/nar/gkh536
- Scopus: eid_2-s2.0-2342460272
- PMID: 15087490
- WOS: WOS:000221145400007
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Article: Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene
Title | Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene |
---|---|
Authors | |
Issue Date | 2004 |
Publisher | Oxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/ |
Citation | Nucleic Acids Research, 2004, v. 32 n. 7, p. 2113-2122 How to Cite? |
Abstract | Adenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5′ splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the intronic part of the stem-loop and demonstrated editing at this site in human brain. We have shown that in cell culture, base substitutions to mimic editing at different combinations of the six sites profoundly affect relative splicing at the normal and the upstream alternative splice site, but splicing at the downstream alternative splice site was consistently rare. Editing combinations in different splice variants from human brain were determined and are consistent with the effects of editing on splicing observed in cell culture. As RNA editing usually occurs close to exon/intron boundaries, this is likely to be a general phenomenon and suggests an important novel role for RNA editing. © Oxford University Press 2004; all rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/175937 |
ISSN | 2023 Impact Factor: 16.6 2023 SCImago Journal Rankings: 7.048 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Flomen, R | en_US |
dc.contributor.author | Knight, J | en_US |
dc.contributor.author | Sham, P | en_US |
dc.contributor.author | Kerwin, R | en_US |
dc.contributor.author | Makoff, A | en_US |
dc.date.accessioned | 2012-11-26T09:02:39Z | - |
dc.date.available | 2012-11-26T09:02:39Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Nucleic Acids Research, 2004, v. 32 n. 7, p. 2113-2122 | en_US |
dc.identifier.issn | 0305-1048 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/175937 | - |
dc.description.abstract | Adenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5′ splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the intronic part of the stem-loop and demonstrated editing at this site in human brain. We have shown that in cell culture, base substitutions to mimic editing at different combinations of the six sites profoundly affect relative splicing at the normal and the upstream alternative splice site, but splicing at the downstream alternative splice site was consistently rare. Editing combinations in different splice variants from human brain were determined and are consistent with the effects of editing on splicing observed in cell culture. As RNA editing usually occurs close to exon/intron boundaries, this is likely to be a general phenomenon and suggests an important novel role for RNA editing. © Oxford University Press 2004; all rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/ | en_US |
dc.relation.ispartof | Nucleic Acids Research | en_US |
dc.subject.mesh | Aged | en_US |
dc.subject.mesh | Aged, 80 And Over | en_US |
dc.subject.mesh | Alternative Splicing - Genetics | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Binding Sites - Genetics | en_US |
dc.subject.mesh | Brain - Metabolism | en_US |
dc.subject.mesh | Cos Cells | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Cell Line, Tumor | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Introns - Genetics | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Models, Genetic | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Mutation | en_US |
dc.subject.mesh | Pc12 Cells | en_US |
dc.subject.mesh | Rna - Genetics - Metabolism | en_US |
dc.subject.mesh | Rna Editing | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Receptor, Serotonin, 5-Ht2c - Genetics | en_US |
dc.title | Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sham, P: pcsham@hku.hk | en_US |
dc.identifier.authority | Sham, P=rp00459 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1093/nar/gkh536 | en_US |
dc.identifier.pmid | 15087490 | - |
dc.identifier.scopus | eid_2-s2.0-2342460272 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-2342460272&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 32 | en_US |
dc.identifier.issue | 7 | en_US |
dc.identifier.spage | 2113 | en_US |
dc.identifier.epage | 2122 | en_US |
dc.identifier.isi | WOS:000221145400007 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Flomen, R=6603223003 | en_US |
dc.identifier.scopusauthorid | Knight, J=13002769800 | en_US |
dc.identifier.scopusauthorid | Sham, P=34573429300 | en_US |
dc.identifier.scopusauthorid | Kerwin, R=7102904567 | en_US |
dc.identifier.scopusauthorid | Makoff, A=7006063526 | en_US |
dc.identifier.issnl | 0305-1048 | - |