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postgraduate thesis: Improving engraftment potential of hMSCs after encapsulation in collagen microsphere: an in vitro and in vivostudy
Title | Improving engraftment potential of hMSCs after encapsulation in collagen microsphere: an in vitro and in vivostudy |
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Authors | |
Advisors | Advisor(s):Chan, BP |
Issue Date | 2012 |
Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
Citation | Wong, M. [王美兒]. (2012). Improving engraftment potential of hMSCs after encapsulation in collagen microsphere : an in vitro and in vivo study. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775308 |
Abstract | Stem cell-based therapies are promising in regenerative medicine. However, the
success of cell therapy is greatly limited by the low engraftment rate to the target
tissues.
The present study demonstrated that human mesenchymal stem cells (hMSCs)
were subjected to a self selection process via microencapsulation in collagen
barrier when they were induced to migrate out from this barrier. While retaining
the immuophenotype and self renewal capacity, the selected hMSCs showed a
significantly better in vitro migratory response of than those cultured in
traditional monolayer. The migratory response could be controlled by varying the
fabrication parameters of the collagen barrier, including initial collagen
concentration and cells seeding density. Affinity to adhere on endothelial cells
layer is another engraftment related property. Significant difference was observed
between these selected hMSCs and hMSCs in monolayer culture.
In order to investigate the engraftment potential of the selected hMSCs, an
animal model was performed. The selected hMSCs were transplanted
intravenously into NOD/SCID mice under partial hepatectomy. Presence of
human cells in the residual liver was determined by the presence of human
HLA-ABC using flow cytometry after 48 hours, 1 week and 1 month.
Engraftment of the selected hMSCs was significantly higher than that of
monolayer cultured hMSCs in time point of 1 month. It demonstrated that the
selected hMSCs favor the engraftment to the injured liver. Further investigation
is required to determine the fate of the engrafted hMSCs in order to truly confirm
their therapeutic potential.
The current work demonstrated that collagen-hMSCs microsphere could act as a
barrier to select hMSCs with enhanced in vitro migratory response and in vivo
engraftment properties. These findings may contribute towards the development
of better stem cell therapies. |
Degree | Master of Philosophy |
Subject | Stem cells - Transplantation. Transplantation of organs, tissues, etc. |
Dept/Program | Mechanical Engineering |
Persistent Identifier | http://hdl.handle.net/10722/174488 |
HKU Library Item ID | b4775308 |
DC Field | Value | Language |
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dc.contributor.advisor | Chan, BP | - |
dc.contributor.author | Wong, Mei-yi. | - |
dc.contributor.author | 王美兒. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Wong, M. [王美兒]. (2012). Improving engraftment potential of hMSCs after encapsulation in collagen microsphere : an in vitro and in vivo study. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775308 | - |
dc.identifier.uri | http://hdl.handle.net/10722/174488 | - |
dc.description.abstract | Stem cell-based therapies are promising in regenerative medicine. However, the success of cell therapy is greatly limited by the low engraftment rate to the target tissues. The present study demonstrated that human mesenchymal stem cells (hMSCs) were subjected to a self selection process via microencapsulation in collagen barrier when they were induced to migrate out from this barrier. While retaining the immuophenotype and self renewal capacity, the selected hMSCs showed a significantly better in vitro migratory response of than those cultured in traditional monolayer. The migratory response could be controlled by varying the fabrication parameters of the collagen barrier, including initial collagen concentration and cells seeding density. Affinity to adhere on endothelial cells layer is another engraftment related property. Significant difference was observed between these selected hMSCs and hMSCs in monolayer culture. In order to investigate the engraftment potential of the selected hMSCs, an animal model was performed. The selected hMSCs were transplanted intravenously into NOD/SCID mice under partial hepatectomy. Presence of human cells in the residual liver was determined by the presence of human HLA-ABC using flow cytometry after 48 hours, 1 week and 1 month. Engraftment of the selected hMSCs was significantly higher than that of monolayer cultured hMSCs in time point of 1 month. It demonstrated that the selected hMSCs favor the engraftment to the injured liver. Further investigation is required to determine the fate of the engrafted hMSCs in order to truly confirm their therapeutic potential. The current work demonstrated that collagen-hMSCs microsphere could act as a barrier to select hMSCs with enhanced in vitro migratory response and in vivo engraftment properties. These findings may contribute towards the development of better stem cell therapies. | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong (Pokfulam, Hong Kong) | - |
dc.relation.ispartof | HKU Theses Online (HKUTO) | - |
dc.rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.source.uri | http://hub.hku.hk/bib/B47753080 | - |
dc.subject.lcsh | Stem cells - Transplantation. | - |
dc.subject.lcsh | Transplantation of organs, tissues, etc. | - |
dc.title | Improving engraftment potential of hMSCs after encapsulation in collagen microsphere: an in vitro and in vivostudy | - |
dc.type | PG_Thesis | - |
dc.identifier.hkul | b4775308 | - |
dc.description.thesisname | Master of Philosophy | - |
dc.description.thesislevel | Master | - |
dc.description.thesisdiscipline | Mechanical Engineering | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.5353/th_b4775308 | - |
dc.date.hkucongregation | 2012 | - |
dc.identifier.mmsid | 991033467619703414 | - |