File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1515/bchm.1998.379.4-5.437
- Scopus: eid_2-s2.0-0031596401
- PMID: 9628335
- WOS: WOS:000073686200010
- Find via
Supplementary
- Citations:
- Appears in Collections:
Conference Paper: Sequence comparison of the EcoHK31 I and EaeI restriction-modification systems suggests an intergenic transfer of genetic material
Title | Sequence comparison of the EcoHK31 I and EaeI restriction-modification systems suggests an intergenic transfer of genetic material |
---|---|
Authors | |
Issue Date | 1998 |
Publisher | Walter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/bc |
Citation | Biological Chemistry, 1998, v. 379 n. 4-5, p. 437-441 How to Cite? |
Abstract | The genes coding for the EcoHK31I and EaeI restriction-modification (R-M) systems from Escherichia coli strain HK31 and Enterobacter aerogenes, respectively, have been cloned and sequenced. Both ENases recognize and cleave Y↓GGCCR leaving 4 nucleotide 5'-protruding ends, while the MTases modify the internal cytosine. The systems were isolated on a 2.3 kb AseI fragment for EcoHK31I, and a 4.6 kb HindIII fragment for EaeI. The R and M genes of both systems converge and overlap by 14 nucleotides. Previously, we found that M.EcoHK31I consisted of two subunits, (α and β), with the β subunit being translated from an alternative open reading frame within the gene encoding the or subunit. Sequence comparison between the EcoHK31I and EaeI systems reveals striking similarity. The eaeIM gene also encodes α and β polypeptides of 309 and 176 amino acids which share 96% and 97% identity, respectively, with those of ecoHK31IM, ecoHK31IR and eaeIR encode proteins of 318 and 315 aa, respectively, which share 92% identity but are otherwise unique in the GenBank database. The EaeI and the EcoHK31I R-M systems were found to be flanked by genes coding for integrases. It is possible that these integrases have facilitated the transfer of this system among different bacterial species. |
Persistent Identifier | http://hdl.handle.net/10722/173566 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 1.172 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, KF | en_US |
dc.contributor.author | Shaw, PC | en_US |
dc.contributor.author | Picone, SJ | en_US |
dc.contributor.author | Wilson, GG | en_US |
dc.contributor.author | Lunnen, KD | en_US |
dc.date.accessioned | 2012-10-30T06:33:24Z | - |
dc.date.available | 2012-10-30T06:33:24Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Biological Chemistry, 1998, v. 379 n. 4-5, p. 437-441 | en_US |
dc.identifier.issn | 1431-6730 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/173566 | - |
dc.description.abstract | The genes coding for the EcoHK31I and EaeI restriction-modification (R-M) systems from Escherichia coli strain HK31 and Enterobacter aerogenes, respectively, have been cloned and sequenced. Both ENases recognize and cleave Y↓GGCCR leaving 4 nucleotide 5'-protruding ends, while the MTases modify the internal cytosine. The systems were isolated on a 2.3 kb AseI fragment for EcoHK31I, and a 4.6 kb HindIII fragment for EaeI. The R and M genes of both systems converge and overlap by 14 nucleotides. Previously, we found that M.EcoHK31I consisted of two subunits, (α and β), with the β subunit being translated from an alternative open reading frame within the gene encoding the or subunit. Sequence comparison between the EcoHK31I and EaeI systems reveals striking similarity. The eaeIM gene also encodes α and β polypeptides of 309 and 176 amino acids which share 96% and 97% identity, respectively, with those of ecoHK31IM, ecoHK31IR and eaeIR encode proteins of 318 and 315 aa, respectively, which share 92% identity but are otherwise unique in the GenBank database. The EaeI and the EcoHK31I R-M systems were found to be flanked by genes coding for integrases. It is possible that these integrases have facilitated the transfer of this system among different bacterial species. | en_US |
dc.language | eng | en_US |
dc.publisher | Walter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/bc | en_US |
dc.relation.ispartof | Biological Chemistry | en_US |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Conjugation, Genetic | en_US |
dc.subject.mesh | Dna, Bacterial | en_US |
dc.subject.mesh | Deoxyribonucleases, Type Ii Site-Specific - Genetics | en_US |
dc.subject.mesh | Enterobacter - Genetics | en_US |
dc.subject.mesh | Escherichia Coli - Genetics | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Recombination, Genetic | en_US |
dc.subject.mesh | Sequence Homology, Amino Acid | en_US |
dc.title | Sequence comparison of the EcoHK31 I and EaeI restriction-modification systems suggests an intergenic transfer of genetic material | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Lee, KF:ckflee@hku.hk | en_US |
dc.identifier.authority | Lee, KF=rp00458 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1515/bchm.1998.379.4-5.437 | - |
dc.identifier.pmid | 9628335 | en_US |
dc.identifier.scopus | eid_2-s2.0-0031596401 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0031596401&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 379 | en_US |
dc.identifier.issue | 4-5 | en_US |
dc.identifier.spage | 437 | en_US |
dc.identifier.epage | 441 | en_US |
dc.identifier.isi | WOS:000073686200010 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Lee, KF=26643097500 | en_US |
dc.identifier.scopusauthorid | Shaw, PC=35599523600 | en_US |
dc.identifier.scopusauthorid | Picone, SJ=7801638815 | en_US |
dc.identifier.scopusauthorid | Wilson, GG=7404529877 | en_US |
dc.identifier.scopusauthorid | Lunnen, KD=6603092080 | en_US |
dc.customcontrol.immutable | sml 170523 amended | - |
dc.identifier.issnl | 1431-6730 | - |