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Article: In vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liver
Title | In vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liver 超順磁性氧化鐵標記大鼠骨髓基質細胞經門靜脈移植MRI活體示蹤的研究 |
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Authors | |
Keywords | Animals, Laboratory Cell Transplantation Magnetic Resonance Imaging Portal Vein Stem Cells |
Issue Date | 2006 |
Publisher | Zhonghua Yixuehui Zazhishe. The Journal's web site is located at http://med.wanfangdata.com.cn/Journal/zhfsx.aspx |
Citation | Chinese Journal Of Radiology, 2006, v. 40 n. 2, p. 133-138 How to Cite? 中華放射學雜誌, 2006, v. 40 n. 2, p. 133-138 How to Cite? |
Abstract | Objective: To evaluate in vivo magnetic resonance imaging with a conventional 1.5-T system for tracking of intra-portal vein transplantation nanometer magnetically labeled BMSCs in rat liver. Methods: BMSCs were isolated from 5 SD rats bone marrow with the density gradient centrifugation method. Then BMSCs were labeled with nanometer superpara-magnetic iron oxide and transfection agent. Cell labeling efficiency was assessed with determination of the percentage of Perls Prussian blue stain. Then BMSCs transplanted into normal rats' livers via portal vein. The receipts were divided into 5 groups, including sham control,2 h,3 d,7 d and 2 w after transplantation. Follow-up serial T1 WI, T2 WI and T2*-weighted gradient-echo MR imaging were performed at 1.5 T MRI system. MR imaging findings were compared with histology. Results: Cell labeling efficiency was more than 95% by Perls Prussian blue stain. After transplantation of labeled BMSCs via portal vein, livers had diffuse granular signal intensity appearance in T2* WI MRI. Cells were detected for up to 2 w in receipts' livers. At histologic analysis, signal intensity loss correlated with iron-loaded cells. Conclusion: MR imaging could aid in monitoring of magnetically labeled BMSCs administered via portal vein in vivo. |
Persistent Identifier | http://hdl.handle.net/10722/172908 |
ISSN | 2023 SCImago Journal Rankings: 0.111 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, P | en_US |
dc.contributor.author | Wang, JH | en_US |
dc.contributor.author | Yan, ZP | en_US |
dc.contributor.author | Hu, MY | en_US |
dc.contributor.author | Xu, PJ | en_US |
dc.contributor.author | Zhou, ML | en_US |
dc.contributor.author | Yan, FH | en_US |
dc.contributor.author | Fan, ST | en_US |
dc.contributor.author | Luk, JM | en_US |
dc.date.accessioned | 2012-10-30T06:25:44Z | - |
dc.date.available | 2012-10-30T06:25:44Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Chinese Journal Of Radiology, 2006, v. 40 n. 2, p. 133-138 | en_US |
dc.identifier.citation | 中華放射學雜誌, 2006, v. 40 n. 2, p. 133-138 | - |
dc.identifier.issn | 1005-1201 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172908 | - |
dc.description.abstract | Objective: To evaluate in vivo magnetic resonance imaging with a conventional 1.5-T system for tracking of intra-portal vein transplantation nanometer magnetically labeled BMSCs in rat liver. Methods: BMSCs were isolated from 5 SD rats bone marrow with the density gradient centrifugation method. Then BMSCs were labeled with nanometer superpara-magnetic iron oxide and transfection agent. Cell labeling efficiency was assessed with determination of the percentage of Perls Prussian blue stain. Then BMSCs transplanted into normal rats' livers via portal vein. The receipts were divided into 5 groups, including sham control,2 h,3 d,7 d and 2 w after transplantation. Follow-up serial T1 WI, T2 WI and T2*-weighted gradient-echo MR imaging were performed at 1.5 T MRI system. MR imaging findings were compared with histology. Results: Cell labeling efficiency was more than 95% by Perls Prussian blue stain. After transplantation of labeled BMSCs via portal vein, livers had diffuse granular signal intensity appearance in T2* WI MRI. Cells were detected for up to 2 w in receipts' livers. At histologic analysis, signal intensity loss correlated with iron-loaded cells. Conclusion: MR imaging could aid in monitoring of magnetically labeled BMSCs administered via portal vein in vivo. | en_US |
dc.language | chi | en_US |
dc.publisher | Zhonghua Yixuehui Zazhishe. The Journal's web site is located at http://med.wanfangdata.com.cn/Journal/zhfsx.aspx | - |
dc.relation.ispartof | Chinese Journal of Radiology | en_US |
dc.relation.ispartof | 中華放射學雜誌 | - |
dc.subject | Animals, Laboratory | en_US |
dc.subject | Cell Transplantation | en_US |
dc.subject | Magnetic Resonance Imaging | en_US |
dc.subject | Portal Vein | en_US |
dc.subject | Stem Cells | en_US |
dc.title | In vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liver | en_US |
dc.title | 超順磁性氧化鐵標記大鼠骨髓基質細胞經門靜脈移植MRI活體示蹤的研究 | - |
dc.type | Article | en_US |
dc.identifier.email | Fan, ST: stfan@hku.hk | en_US |
dc.identifier.email | Luk, JM: jmluk@hkucc.hku.hk | en_US |
dc.identifier.authority | Fan, ST=rp00355 | en_US |
dc.identifier.authority | Luk, JM=rp00349 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.3760/j.issn:1005-1201.2006.02.005 | - |
dc.identifier.scopus | eid_2-s2.0-33645546579 | en_US |
dc.identifier.hkuros | 137565 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33645546579&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 40 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 133 | en_US |
dc.identifier.epage | 138 | en_US |
dc.publisher.place | China | en_US |
dc.identifier.scopusauthorid | Wang, P=7405460611 | en_US |
dc.identifier.scopusauthorid | Wang, JH=8898010700 | en_US |
dc.identifier.scopusauthorid | Yan, ZP=7402519656 | en_US |
dc.identifier.scopusauthorid | Hu, MY=7402639252 | en_US |
dc.identifier.scopusauthorid | Xu, PJ=7202215783 | en_US |
dc.identifier.scopusauthorid | Zhou, ML=7403506132 | en_US |
dc.identifier.scopusauthorid | Yan, FH=12789764400 | en_US |
dc.identifier.scopusauthorid | Fan, ST=7402678224 | en_US |
dc.identifier.scopusauthorid | Luk, JM=7006777791 | en_US |
dc.identifier.issnl | 1005-1201 | - |