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Article: Rapid and sensitive detection of Salmonella (O:6,7) by immunomagnetic monoclonal antibody-based assays

TitleRapid and sensitive detection of Salmonella (O:6,7) by immunomagnetic monoclonal antibody-based assays
Authors
Luk, JMCLindberg, AA
KeywordsEnzyme immunoassay
Magnetic bead
Monoclonal antibody
Salmonella O:6,7 lipopolysaccharide
Issue Date1991
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
Citation
Journal Of Immunological Methods, 1991, v. 137 n. 1, p. 1-8 How to Cite?
DOI: http://dx.doi.org/10.1016/0022-1759(91)90387-U
AbstractAn immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange staining and measured by enzyme immunoassays with a conjugate anti-LPS MAb as the detector probe. The whole detection process required 2-3 h and the sensitivity was 103-104 bacteria/ml. The presence of blood (10%, v/v) or stool (1%, w/v) components did not interfere with the immunomagnetic assay performance.
Persistent Identifierhttp://hdl.handle.net/10722/172655
ISSN
0022-1759
2023 Impact Factor: 1.6
2023 SCImago Journal Rankings: 0.555
ISI Accession Number ID
WOS:A1991FB24000001

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorLindberg, AAen_US
dc.date.accessioned2012-10-30T06:24:03Z-
dc.date.available2012-10-30T06:24:03Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of Immunological Methods, 1991, v. 137 n. 1, p. 1-8en_US
dc.identifier.issn0022-1759en_US
dc.identifier.urihttp://hdl.handle.net/10722/172655-
dc.description.abstractAn immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange staining and measured by enzyme immunoassays with a conjugate anti-LPS MAb as the detector probe. The whole detection process required 2-3 h and the sensitivity was 103-104 bacteria/ml. The presence of blood (10%, v/v) or stool (1%, w/v) components did not interfere with the immunomagnetic assay performance.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jimen_US
dc.relation.ispartofJournal of Immunological Methodsen_US
dc.subjectEnzyme immunoassay-
dc.subjectMagnetic bead-
dc.subjectMonoclonal antibody-
dc.subjectSalmonella O:6,7 lipopolysaccharide-
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Diagnostic Useen_US
dc.subject.meshAntigens, Bacterial - Analysis - Immunologyen_US
dc.subject.meshFeces - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshLatex Fixation Testsen_US
dc.subject.meshMagneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshO Antigensen_US
dc.subject.meshSalmonella - Immunology - Isolation & Purificationen_US
dc.titleRapid and sensitive detection of Salmonella (O:6,7) by immunomagnetic monoclonal antibody-based assaysen_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0022-1759(91)90387-U-
dc.identifier.pmid1707080-
dc.identifier.scopuseid_2-s2.0-0026034328en_US
dc.identifier.volume137en_US
dc.identifier.issue1en_US
dc.identifier.spage1en_US
dc.identifier.epage8en_US
dc.identifier.isiWOS:A1991FB24000001-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridLindberg, AA=7101776377en_US
dc.identifier.issnl0022-1759-

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