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- Publisher Website: 10.1099/00222615-26-2-115
- Scopus: eid_2-s2.0-0023909159
- PMID: 2455055
- WOS: WOS:A1988N885700006
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Article: Characterisation and application of a murine monoclonal antibody specific for the serogroup C2 Salmonella
Title | Characterisation and application of a murine monoclonal antibody specific for the serogroup C2 Salmonella |
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Authors | |
Issue Date | 1988 |
Publisher | Society for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org |
Citation | Journal Of Medical Microbiology, 1988, v. 26 n. 2, p. 115-119 How to Cite? |
Abstract | An IgG3 murine monoclonal antibody (designated MO8) specific for the serogroup C2 Salmonella lipopolysaccharide (LPS) was generated by fusing mouse myeloma cells NS1 with spleen cells of BALB/c mice immunised with heat-killed S. manhattan. MO8 reacted with purified LPS prepared from serogroup C2 Salmonella but did not react with that prepared from other O serogroups, and its reactivity was also specifically absorbed by serogroup C2 Salmonella only. Polyacrylamide gel electrophoresis of the serogroup C2 LPS and subsequent immunoblotting with MO8 yielded multiple reactive bands giving a characteristic ladder pattern. The specificity of MO8 was further demonstrated in the slide agglutination test with 223 bacteria, of which only 25 belonging to serogroup C2 Salmonella reacted with the MO8 ascitic fluid. The specificity of MO8 makes it useful not only for the serological identification of Salmonella but also for the epitope analysis of the serogroup C2 LPS. |
Persistent Identifier | http://hdl.handle.net/10722/172585 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.752 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Luk, JMC | en_US |
dc.contributor.author | Chan, KH | en_US |
dc.contributor.author | Tsang, RSW | en_US |
dc.contributor.author | Ng, MH | en_US |
dc.date.accessioned | 2012-10-30T06:23:33Z | - |
dc.date.available | 2012-10-30T06:23:33Z | - |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | Journal Of Medical Microbiology, 1988, v. 26 n. 2, p. 115-119 | en_US |
dc.identifier.issn | 0022-2615 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172585 | - |
dc.description.abstract | An IgG3 murine monoclonal antibody (designated MO8) specific for the serogroup C2 Salmonella lipopolysaccharide (LPS) was generated by fusing mouse myeloma cells NS1 with spleen cells of BALB/c mice immunised with heat-killed S. manhattan. MO8 reacted with purified LPS prepared from serogroup C2 Salmonella but did not react with that prepared from other O serogroups, and its reactivity was also specifically absorbed by serogroup C2 Salmonella only. Polyacrylamide gel electrophoresis of the serogroup C2 LPS and subsequent immunoblotting with MO8 yielded multiple reactive bands giving a characteristic ladder pattern. The specificity of MO8 was further demonstrated in the slide agglutination test with 223 bacteria, of which only 25 belonging to serogroup C2 Salmonella reacted with the MO8 ascitic fluid. The specificity of MO8 makes it useful not only for the serological identification of Salmonella but also for the epitope analysis of the serogroup C2 LPS. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org | en_US |
dc.relation.ispartof | Journal of Medical Microbiology | en_US |
dc.subject.mesh | Agglutination Tests | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Biosynthesis - Immunology | en_US |
dc.subject.mesh | Antibody Specificity | en_US |
dc.subject.mesh | Antigens, Surface - Immunology | en_US |
dc.subject.mesh | Binding, Competitive | en_US |
dc.subject.mesh | Electrophoresis, Polyacrylamide Gel | en_US |
dc.subject.mesh | Epitopes - Immunology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Hybridomas | en_US |
dc.subject.mesh | Immunoassay | en_US |
dc.subject.mesh | Immunoglobulin G - Immunology | en_US |
dc.subject.mesh | Lipopolysaccharides - Immunology | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Salmonella - Immunology | en_US |
dc.title | Characterisation and application of a murine monoclonal antibody specific for the serogroup C2 Salmonella | en_US |
dc.type | Article | en_US |
dc.identifier.email | Luk, JMC: jmluk@hkucc.hku.hk | en_US |
dc.identifier.authority | Luk, JMC=rp00349 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1099/00222615-26-2-115 | - |
dc.identifier.pmid | 2455055 | - |
dc.identifier.scopus | eid_2-s2.0-0023909159 | en_US |
dc.identifier.volume | 26 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 115 | en_US |
dc.identifier.epage | 119 | en_US |
dc.identifier.isi | WOS:A1988N885700006 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Luk, JMC=7006777791 | en_US |
dc.identifier.scopusauthorid | Chan, KH=35338760600 | en_US |
dc.identifier.scopusauthorid | Tsang, RSW=7102940066 | en_US |
dc.identifier.scopusauthorid | Ng, MH=7202076421 | en_US |
dc.identifier.issnl | 0022-2615 | - |