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- Publisher Website: 10.1016/S0024-3205(99)00152-6
- Scopus: eid_2-s2.0-0033597342
- PMID: 10374930
- WOS: WOS:000080107200009
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Article: Two distinct pathways for refilling Ca2+ stores in permeabilized bovine trachealis muscle
Title | Two distinct pathways for refilling Ca2+ stores in permeabilized bovine trachealis muscle |
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Authors | |
Keywords | Bovine tracheal smooth muscle Calcium Dihydropyridine-sensitive pathway Sarcoplasmic reticulum |
Issue Date | 1999 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie |
Citation | Life Sciences, 1999, v. 64 n. 22, p. 2049-2059 How to Cite? |
Abstract | Calcium entry from extracellular space to acetylcholine (ACh)-sensitive internal stores was investigated in β-escin permeabilized bovine tracheal smooth muscle. Cyclopiazonic acid (CPA), a selective inhibitor of the sarcoplasmic reticulum (SR) calcium pump, and nifedipine, both inhibited the refilling, and inhibition was larger when these compounds were used simultaneously. BayK 8644 enhanced the refilling and completely reversed the inhibition induced by cyclopiazonic acid. In pCa 7 solution containing CPA, there was a spontaneous time-dependent decrease of ACh-induced transient contraction. In the presence of nifedipine or verapamil in the incubation solution reduced this time-dependent decrease in contractile responses to ACh stimulation, suggesting that these calcium-entry blockers decreased calcium leakage from internal stores to the extracellular space. These results suggest that in addition to the active calcium uptake in the SR, another pathway controlled by an L-type like calcium channel (dihydropyridine- sensitive) may exist between the extracellular compartment and the lumen of the SR in airway smooth muscle, and contributes significantly to the loading of ACh-sensitive calcium stores. |
Persistent Identifier | http://hdl.handle.net/10722/171672 |
ISSN | 2023 Impact Factor: 5.2 2023 SCImago Journal Rankings: 1.257 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Qian, Y | en_US |
dc.contributor.author | Bourreau, JP | en_US |
dc.date.accessioned | 2012-10-30T06:16:16Z | - |
dc.date.available | 2012-10-30T06:16:16Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Life Sciences, 1999, v. 64 n. 22, p. 2049-2059 | en_US |
dc.identifier.issn | 0024-3205 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171672 | - |
dc.description.abstract | Calcium entry from extracellular space to acetylcholine (ACh)-sensitive internal stores was investigated in β-escin permeabilized bovine tracheal smooth muscle. Cyclopiazonic acid (CPA), a selective inhibitor of the sarcoplasmic reticulum (SR) calcium pump, and nifedipine, both inhibited the refilling, and inhibition was larger when these compounds were used simultaneously. BayK 8644 enhanced the refilling and completely reversed the inhibition induced by cyclopiazonic acid. In pCa 7 solution containing CPA, there was a spontaneous time-dependent decrease of ACh-induced transient contraction. In the presence of nifedipine or verapamil in the incubation solution reduced this time-dependent decrease in contractile responses to ACh stimulation, suggesting that these calcium-entry blockers decreased calcium leakage from internal stores to the extracellular space. These results suggest that in addition to the active calcium uptake in the SR, another pathway controlled by an L-type like calcium channel (dihydropyridine- sensitive) may exist between the extracellular compartment and the lumen of the SR in airway smooth muscle, and contributes significantly to the loading of ACh-sensitive calcium stores. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie | en_US |
dc.relation.ispartof | Life Sciences | en_US |
dc.rights | Life Sciences. Copyright © Elsevier Inc. | - |
dc.subject | Bovine tracheal smooth muscle | - |
dc.subject | Calcium | - |
dc.subject | Dihydropyridine-sensitive pathway | - |
dc.subject | Sarcoplasmic reticulum | - |
dc.subject.mesh | Acetylcholine - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Calcium - Metabolism | en_US |
dc.subject.mesh | Calcium Channel Blockers - Pharmacology | en_US |
dc.subject.mesh | Calcium Channels - Metabolism | en_US |
dc.subject.mesh | Calcium Signaling - Drug Effects | en_US |
dc.subject.mesh | Calcium-Transporting Atpases - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Cattle | en_US |
dc.subject.mesh | Cell Membrane Permeability - Drug Effects | en_US |
dc.subject.mesh | Enzyme Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Escin - Pharmacology | en_US |
dc.subject.mesh | Indoles - Pharmacology | en_US |
dc.subject.mesh | Ion Transport - Drug Effects | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects | en_US |
dc.subject.mesh | Muscle, Smooth - Drug Effects - Metabolism - Physiology | en_US |
dc.subject.mesh | Nifedipine - Pharmacology | en_US |
dc.subject.mesh | Sarcoplasmic Reticulum - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Trachea - Drug Effects - Metabolism - Physiology | en_US |
dc.subject.mesh | Verapamil - Pharmacology | en_US |
dc.title | Two distinct pathways for refilling Ca2+ stores in permeabilized bovine trachealis muscle | en_US |
dc.type | Article | en_US |
dc.identifier.email | Bourreau, JP:bourreau@hkucc.hku.hk | en_US |
dc.identifier.authority | Bourreau, JP=rp00389 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0024-3205(99)00152-6 | en_US |
dc.identifier.pmid | 10374930 | - |
dc.identifier.scopus | eid_2-s2.0-0033597342 | en_US |
dc.identifier.hkuros | 44391 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033597342&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 64 | en_US |
dc.identifier.issue | 22 | en_US |
dc.identifier.spage | 2049 | en_US |
dc.identifier.epage | 2059 | en_US |
dc.identifier.isi | WOS:000080107200009 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Qian, Y=37019487300 | en_US |
dc.identifier.scopusauthorid | Bourreau, JP=7003927886 | en_US |
dc.identifier.issnl | 0024-3205 | - |