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- Publisher Website: 10.1099/0022-1317-72-6-1451
- Scopus: eid_2-s2.0-0025869332
- PMID: 1710651
- WOS: WOS:A1991FQ45300032
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Article: 17D yellow fever vaccine virus envelope protein expressed by recombinant baculovirus is antigenically indistinguishable from authentic viral protein
Title | 17D yellow fever vaccine virus envelope protein expressed by recombinant baculovirus is antigenically indistinguishable from authentic viral protein |
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Authors | |
Issue Date | 1991 |
Publisher | Society for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org |
Citation | Journal Of General Virology, 1991, v. 72 n. 6, p. 1451-1454 How to Cite? |
Abstract | We have constructed a recombinant baculovirus containing cloned DNA encoding the membrane and envelope (E) proteins of 17D yellow fever vaccine virus. Spodoptera frugiperda cells infected with this recombinant baculovirus produced a 66K protein which corresponded to the estimated size of the protein encoded by the cloned inserted DNA, and a 54K protein with the same molecular size as that of the authentic 17D yellow fever virus E protein. This recombinant 54K protein was labile, producing E protein-specific breakdown products (45K to 36K). Indirect immunofluorescence, using a panel of E protein-specific monoclonal antibodies, showed that the recombinant protein was presented both inside as well as on the surface of cells and was antigenically indistinguishable from the E protein of 17D yellow fever vaccine virus. |
Persistent Identifier | http://hdl.handle.net/10722/171554 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 0.990 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shiu, SYW | en_US |
dc.contributor.author | Morikawa, S | en_US |
dc.contributor.author | Buckley, A | en_US |
dc.contributor.author | Higgs, S | en_US |
dc.contributor.author | Karunakarannair, V | en_US |
dc.contributor.author | Blachere, C | en_US |
dc.contributor.author | Gould, EA | en_US |
dc.date.accessioned | 2012-10-30T06:15:39Z | - |
dc.date.available | 2012-10-30T06:15:39Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Journal Of General Virology, 1991, v. 72 n. 6, p. 1451-1454 | en_US |
dc.identifier.issn | 0022-1317 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171554 | - |
dc.description.abstract | We have constructed a recombinant baculovirus containing cloned DNA encoding the membrane and envelope (E) proteins of 17D yellow fever vaccine virus. Spodoptera frugiperda cells infected with this recombinant baculovirus produced a 66K protein which corresponded to the estimated size of the protein encoded by the cloned inserted DNA, and a 54K protein with the same molecular size as that of the authentic 17D yellow fever virus E protein. This recombinant 54K protein was labile, producing E protein-specific breakdown products (45K to 36K). Indirect immunofluorescence, using a panel of E protein-specific monoclonal antibodies, showed that the recombinant protein was presented both inside as well as on the surface of cells and was antigenically indistinguishable from the E protein of 17D yellow fever vaccine virus. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org | en_US |
dc.relation.ispartof | Journal of General Virology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Diagnostic Use | en_US |
dc.subject.mesh | Baculoviridae - Genetics | en_US |
dc.subject.mesh | Capsid - Genetics | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Dna, Viral - Genetics | en_US |
dc.subject.mesh | Epitopes - Analysis | en_US |
dc.subject.mesh | Fluorescent Antibody Technique | en_US |
dc.subject.mesh | Genetic Vectors | en_US |
dc.subject.mesh | Immunoblotting | en_US |
dc.subject.mesh | Molecular Weight | en_US |
dc.subject.mesh | Moths | en_US |
dc.subject.mesh | Recombinant Proteins - Immunology | en_US |
dc.subject.mesh | Restriction Mapping | en_US |
dc.subject.mesh | Vaccines, Synthetic | en_US |
dc.subject.mesh | Viral Core Proteins - Genetics | en_US |
dc.subject.mesh | Viral Envelope Proteins - Genetics - Immunology | en_US |
dc.subject.mesh | Viral Nonstructural Proteins | en_US |
dc.subject.mesh | Viral Structural Proteins - Genetics | en_US |
dc.subject.mesh | Viral Vaccines | en_US |
dc.subject.mesh | Yellow Fever Virus - Genetics - Immunology | en_US |
dc.title | 17D yellow fever vaccine virus envelope protein expressed by recombinant baculovirus is antigenically indistinguishable from authentic viral protein | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shiu, SYW:sywshiu@hkucc.hku.hk | en_US |
dc.identifier.authority | Shiu, SYW=rp00384 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1099/0022-1317-72-6-1451 | - |
dc.identifier.pmid | 1710651 | - |
dc.identifier.scopus | eid_2-s2.0-0025869332 | en_US |
dc.identifier.volume | 72 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 1451 | en_US |
dc.identifier.epage | 1454 | en_US |
dc.identifier.isi | WOS:A1991FQ45300032 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Shiu, SYW=7005550655 | en_US |
dc.identifier.scopusauthorid | Morikawa, S=7102226341 | en_US |
dc.identifier.scopusauthorid | Buckley, A=7102458647 | en_US |
dc.identifier.scopusauthorid | Higgs, S=34975082700 | en_US |
dc.identifier.scopusauthorid | Karunakarannair, V=6504279759 | en_US |
dc.identifier.scopusauthorid | Blachere, C=55283749000 | en_US |
dc.identifier.scopusauthorid | Gould, EA=7101857623 | en_US |
dc.identifier.issnl | 0022-1317 | - |