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Article: Potassium ions and endothelium-derived hyperpolarizing factor in guinea-pig carotid and porcine coronary arteries
Title | Potassium ions and endothelium-derived hyperpolarizing factor in guinea-pig carotid and porcine coronary arteries |
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Authors | |
Keywords | Barium EDHF Endothelium Endothelium-derived hyperpolarizing factor Inwardly rectifying potassium current Na+/K+ pump Ouabain Potassium Vascular smooth muscle cells |
Issue Date | 1999 |
Publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 |
Citation | British Journal Of Pharmacology, 1999, v. 127 n. 1, p. 27-34 How to Cite? |
Abstract | 1. Experiments were designed to determine in two arteries (the guinea-pig carotid and the porcine coronary arteries) whether or not the endothelium-derived hyperpolarizing factor (EDHF) can be identified as potassium ions, and to determine whether or not the inwardly rectifying potassium current and the Na+/K+ pump are involved in the hyperpolarization mediated by EDHF. 2. The membrane potential of vascular smooth muscle cells was recorded with intracellular microelectrodes in the presence of N(ω)-L-nitro-arginine (L-NA) and indomethacin. 3. In vascular smooth muscle cells of guinea-pig carotid and porcine coronary arteries, acetylcholine and bradykinin induced endothelium-dependent hyperpolarizations (-18 ± 1 mV, n = 39 and -19 ± 1 mV, n = 7, respectively). The hyperpolarizations were not affected significantly by ouabain (1 μM), barium chloride (up to 100 μM) or the combination of ouabain plus barium. 4. In both arteries, increasing extracellular potassium concentration by 5 or 10 mM induced either depolarization or in a very few cases small hyperpolarizations which never exceeded 2 mV. 5. In isolated smooth muscle cells of the guinea-pig carotid artery, patch-clamp experiments shows that only 20% of the vascular smooth muscle cells expressed inwardly rectifying potassium channels. The current density recorded was low (0.5 ± 0.1 pA pF-1, n = 8). 6. These results indicate that, in two different vascular preparations, barium sensitive-inwardly rectifying potassium conductance and the ouabain sensitive-Na+/K+ pump are not involved in the EDHF-mediated hyperpolarization. Furthermore, potassium did not mimic the effect of EDHF pointing out that potassium and EDHF are not the same entity in those arteries. |
Persistent Identifier | http://hdl.handle.net/10722/171224 |
ISSN | 2023 Impact Factor: 6.8 2023 SCImago Journal Rankings: 2.119 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Quignard, JF | en_US |
dc.contributor.author | Félétou, M | en_US |
dc.contributor.author | Thollon, C | en_US |
dc.contributor.author | Vilaine, JP | en_US |
dc.contributor.author | Duhault, J | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:12:48Z | - |
dc.date.available | 2012-10-30T06:12:48Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | British Journal Of Pharmacology, 1999, v. 127 n. 1, p. 27-34 | en_US |
dc.identifier.issn | 0007-1188 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171224 | - |
dc.description.abstract | 1. Experiments were designed to determine in two arteries (the guinea-pig carotid and the porcine coronary arteries) whether or not the endothelium-derived hyperpolarizing factor (EDHF) can be identified as potassium ions, and to determine whether or not the inwardly rectifying potassium current and the Na+/K+ pump are involved in the hyperpolarization mediated by EDHF. 2. The membrane potential of vascular smooth muscle cells was recorded with intracellular microelectrodes in the presence of N(ω)-L-nitro-arginine (L-NA) and indomethacin. 3. In vascular smooth muscle cells of guinea-pig carotid and porcine coronary arteries, acetylcholine and bradykinin induced endothelium-dependent hyperpolarizations (-18 ± 1 mV, n = 39 and -19 ± 1 mV, n = 7, respectively). The hyperpolarizations were not affected significantly by ouabain (1 μM), barium chloride (up to 100 μM) or the combination of ouabain plus barium. 4. In both arteries, increasing extracellular potassium concentration by 5 or 10 mM induced either depolarization or in a very few cases small hyperpolarizations which never exceeded 2 mV. 5. In isolated smooth muscle cells of the guinea-pig carotid artery, patch-clamp experiments shows that only 20% of the vascular smooth muscle cells expressed inwardly rectifying potassium channels. The current density recorded was low (0.5 ± 0.1 pA pF-1, n = 8). 6. These results indicate that, in two different vascular preparations, barium sensitive-inwardly rectifying potassium conductance and the ouabain sensitive-Na+/K+ pump are not involved in the EDHF-mediated hyperpolarization. Furthermore, potassium did not mimic the effect of EDHF pointing out that potassium and EDHF are not the same entity in those arteries. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 | en_US |
dc.relation.ispartof | British Journal of Pharmacology | en_US |
dc.subject | Barium | - |
dc.subject | EDHF | - |
dc.subject | Endothelium | - |
dc.subject | Endothelium-derived hyperpolarizing factor | - |
dc.subject | Inwardly rectifying potassium current | - |
dc.subject | Na+/K+ pump | - |
dc.subject | Ouabain | - |
dc.subject | Potassium | - |
dc.subject | Vascular smooth muscle cells | - |
dc.subject.mesh | Acetylcholine - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Biological Factors - Metabolism | en_US |
dc.subject.mesh | Carotid Arteries - Metabolism | en_US |
dc.subject.mesh | Coronary Vessels - Metabolism | en_US |
dc.subject.mesh | Guinea Pigs | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Potentials | en_US |
dc.subject.mesh | Microelectrodes | en_US |
dc.subject.mesh | Patch-Clamp Techniques | en_US |
dc.subject.mesh | Potassium - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Potassium Channels - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Swine | en_US |
dc.title | Potassium ions and endothelium-derived hyperpolarizing factor in guinea-pig carotid and porcine coronary arteries | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1038/sj.bjp.0702493 | en_US |
dc.identifier.pmid | 10369452 | - |
dc.identifier.scopus | eid_2-s2.0-0032938529 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032938529&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 127 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 27 | en_US |
dc.identifier.epage | 34 | en_US |
dc.identifier.isi | WOS:000080097400004 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Quignard, JF=13606215800 | en_US |
dc.identifier.scopusauthorid | Félétou, M=7006461826 | en_US |
dc.identifier.scopusauthorid | Thollon, C=6602540205 | en_US |
dc.identifier.scopusauthorid | Vilaine, JP=7004617134 | en_US |
dc.identifier.scopusauthorid | Duhault, J=7005108808 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0007-1188 | - |