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- Publisher Website: 10.1038/sj.bjp.0701629
- Scopus: eid_2-s2.0-0031984877
- PMID: 9504399
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Article: Epoxyeicosatrienoic acids, potassium channel blockers and endothelium-dependent hyperpolarization in the guinea-pig carotid artery
Title | Epoxyeicosatrienoic acids, potassium channel blockers and endothelium-dependent hyperpolarization in the guinea-pig carotid artery |
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Authors | |
Keywords | 17-ODYA Acetylcholine Cytochrome P450 EDHF Endothelium Epoxyeicosatrienoic acids Hyperpolarization Iberiotoxin Smooth muscle |
Issue Date | 1998 |
Publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 |
Citation | British Journal Of Pharmacology, 1998, v. 123 n. 3, p. 574-580 How to Cite? |
Abstract | 1. Using intracellular microelectrodes, we investigated the effects of 17-octadecynoic acid (17-ODYA) on the endothelium-dependent hyperpolarization induced by acetylcholine in the guinea-pig isolated internal carotid artery with endothelium. 2. In the presence of N(ω)-nitro-L-arginine (L-NOARG, 100 μM) and indomethacin (5 μM) to inhibit nitric oxide synthase and cyclo-oxygenase, acetylcholine (1 μM) evoked an endothelium-dependent hyperpolarization which averaged -16.4 mV starting from a resting membrane potential of -56.8 mV. There was a negative correlation between the amplitude of the hyperpolarization and the absolute values of the resting membrane potential. 3. The acetylcholine-induced endothelium-dependent hyperpolarization was not altered by charybdotoxin (0.1 μM) or iberiotoxin (30 nM). It was partially but significantly reduced by apamin (0.5 μM) to -12.8 ± 1.2 mV (n = 10) or the combination of apamin plus iberiotoxin (-14.3 ± 3.4 mV, n = 4). However, the combination of charybdotoxin and apamin abolished the hyperpolarization and under these conditions, acetylcholine evoked a depolarization (+7.1 ± 3.7 mV, n = 8). 4. 17-ODYA (10 μM) produced a significant hyperpolarization of the resting membrane potential which averaged -59.6 mV and a partial but significant inhibition of the acetylcholine-induced endothelium-dependent hyperpolarization (-10.9 mV). 5. Apamin did not modify the effects of 17-ODYA but in the presence of charybdotoxin or iberiotoxin, 17-ODYA no longer influenced the resting membrane potential or the acetylcholine-induced hyperpolarization. 6. When compared to solvent (ethanol, 1% v/v), epoxyeicosatrienoic acids (EpETrEs) (5,6-, 8,9-, 11,12- and 14,15-EpETrE, 3 μM) did not affect the cell membrane potential and did not relax the guinea-pig isolated internal carotid artery. 7. These results indicate that, in the guinea-pig internal carotid artery, the involvement of metabolites of arachidonic acid through the cytochrome P450 pathway in endothelium-dependent hyperpolarization is unlikely. Furthermore, the hyperpolarization mediated by the endothelium-derived hyperpolarizing factor (EDHF) is probably not due to the opening of BK(Ca) channels. |
Persistent Identifier | http://hdl.handle.net/10722/171211 |
ISSN | 2023 Impact Factor: 6.8 2023 SCImago Journal Rankings: 2.119 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chataigneau, T | en_US |
dc.contributor.author | Félétou, M | en_US |
dc.contributor.author | Duhault, J | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:12:43Z | - |
dc.date.available | 2012-10-30T06:12:43Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | British Journal Of Pharmacology, 1998, v. 123 n. 3, p. 574-580 | en_US |
dc.identifier.issn | 0007-1188 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171211 | - |
dc.description.abstract | 1. Using intracellular microelectrodes, we investigated the effects of 17-octadecynoic acid (17-ODYA) on the endothelium-dependent hyperpolarization induced by acetylcholine in the guinea-pig isolated internal carotid artery with endothelium. 2. In the presence of N(ω)-nitro-L-arginine (L-NOARG, 100 μM) and indomethacin (5 μM) to inhibit nitric oxide synthase and cyclo-oxygenase, acetylcholine (1 μM) evoked an endothelium-dependent hyperpolarization which averaged -16.4 mV starting from a resting membrane potential of -56.8 mV. There was a negative correlation between the amplitude of the hyperpolarization and the absolute values of the resting membrane potential. 3. The acetylcholine-induced endothelium-dependent hyperpolarization was not altered by charybdotoxin (0.1 μM) or iberiotoxin (30 nM). It was partially but significantly reduced by apamin (0.5 μM) to -12.8 ± 1.2 mV (n = 10) or the combination of apamin plus iberiotoxin (-14.3 ± 3.4 mV, n = 4). However, the combination of charybdotoxin and apamin abolished the hyperpolarization and under these conditions, acetylcholine evoked a depolarization (+7.1 ± 3.7 mV, n = 8). 4. 17-ODYA (10 μM) produced a significant hyperpolarization of the resting membrane potential which averaged -59.6 mV and a partial but significant inhibition of the acetylcholine-induced endothelium-dependent hyperpolarization (-10.9 mV). 5. Apamin did not modify the effects of 17-ODYA but in the presence of charybdotoxin or iberiotoxin, 17-ODYA no longer influenced the resting membrane potential or the acetylcholine-induced hyperpolarization. 6. When compared to solvent (ethanol, 1% v/v), epoxyeicosatrienoic acids (EpETrEs) (5,6-, 8,9-, 11,12- and 14,15-EpETrE, 3 μM) did not affect the cell membrane potential and did not relax the guinea-pig isolated internal carotid artery. 7. These results indicate that, in the guinea-pig internal carotid artery, the involvement of metabolites of arachidonic acid through the cytochrome P450 pathway in endothelium-dependent hyperpolarization is unlikely. Furthermore, the hyperpolarization mediated by the endothelium-derived hyperpolarizing factor (EDHF) is probably not due to the opening of BK(Ca) channels. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1 | en_US |
dc.relation.ispartof | British Journal of Pharmacology | en_US |
dc.subject | 17-ODYA | - |
dc.subject | Acetylcholine | - |
dc.subject | Cytochrome P450 | - |
dc.subject | EDHF | - |
dc.subject | Endothelium | - |
dc.subject | Epoxyeicosatrienoic acids | - |
dc.subject | Hyperpolarization | - |
dc.subject | Iberiotoxin | - |
dc.subject | Smooth muscle | - |
dc.subject.mesh | 8,11,14-Eicosatrienoic Acid - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Carotid Arteries - Drug Effects - Physiology | en_US |
dc.subject.mesh | Charybdotoxin - Pharmacology | en_US |
dc.subject.mesh | Cytochrome P-450 Enzyme System - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Electromyography | en_US |
dc.subject.mesh | Endothelium, Vascular - Drug Effects - Physiology | en_US |
dc.subject.mesh | Enzyme Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Fatty Acids, Unsaturated - Pharmacology | en_US |
dc.subject.mesh | Guinea Pigs | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Potentials - Drug Effects | en_US |
dc.subject.mesh | Peptides - Pharmacology | en_US |
dc.subject.mesh | Potassium Channel Blockers | en_US |
dc.title | Epoxyeicosatrienoic acids, potassium channel blockers and endothelium-dependent hyperpolarization in the guinea-pig carotid artery | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1038/sj.bjp.0701629 | en_US |
dc.identifier.pmid | 9504399 | - |
dc.identifier.scopus | eid_2-s2.0-0031984877 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0031984877&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 123 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 574 | en_US |
dc.identifier.epage | 580 | en_US |
dc.identifier.isi | WOS:000071891100026 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Chataigneau, T=6602561430 | en_US |
dc.identifier.scopusauthorid | Félétou, M=7006461826 | en_US |
dc.identifier.scopusauthorid | Duhault, J=7005108808 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0007-1188 | - |