File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1203/00006450-199108000-00011
- Scopus: eid_2-s2.0-0026350137
- PMID: 1896263
- WOS: WOS:A1991FY61100010
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Endothelium-dependent relaxations of piglet pulmonary arteries augment with maturation
Title | Endothelium-dependent relaxations of piglet pulmonary arteries augment with maturation |
---|---|
Authors | |
Issue Date | 1991 |
Publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/ |
Citation | Pediatric Research, 1991, v. 30 n. 2, p. 176-180 How to Cite? |
Abstract | To determine whether maturation alters endothelium-dependent responses in porcine pulmonary arteries, rings, with and without endothelium, of small pulmonary arteries taken from piglets of 3, 10, and 30 d of age were suspended in organ chambers filled with buffered salt solution, bubbled with 95% O2-5% CO2, and maintained at 37°C. These studies were performed in the presence of indomethacin (10-5 M) to inhibit prostaglandin synthesis. In rings without endothelium, potassium chloride (10-2 to 8.5 x 10-2 M) and histamine (10-9 to 10-5 M) caused concentration-dependent contractions. When normalized to maximal contractions achieved to each agonist, the concentration-effect curves to potassium chloride and histamine in rings without endothelium were similar at each age. Rings with endothelium showed a progressive shift to the right of the concentration-effect curve to histamine, possibly secondary to an increase in the basal release of, or responsiveness to, the endothelium-derived relaxing factor with maturation. Relaxations to sodium nitroprusside (10-9 to 10-5 M) were unaffected by age. In precontracted rings, acetylcholine (10-9 to 10-6 M), bradykinin (10-10 to 10-6 M), and the calcium ionophore A23187 (10-9 to 10-6 M) caused relaxations in rings with endothelium, but not in those without endothelium, which were greater at 10 and 30 d compared to 3 d; further augmentation at 30 d compared to 10 d was not observed. In rings without endothelium, changes in the responsiveness to nitric oxide (10-9 to 10-5 M), one of the proposed endothelium-derived relaxing factors, with age were comparable to those observed with endothelium-dependent relaxing agents. These studies demonstrate that endothelium-dependent relaxations increase with age, possibly due to changes in sensitivity of the smooth muscle to the endothelium-derived relaxing factor. |
Persistent Identifier | http://hdl.handle.net/10722/171042 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.184 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zellers, TM | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:11:57Z | - |
dc.date.available | 2012-10-30T06:11:57Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Pediatric Research, 1991, v. 30 n. 2, p. 176-180 | en_US |
dc.identifier.issn | 0031-3998 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171042 | - |
dc.description.abstract | To determine whether maturation alters endothelium-dependent responses in porcine pulmonary arteries, rings, with and without endothelium, of small pulmonary arteries taken from piglets of 3, 10, and 30 d of age were suspended in organ chambers filled with buffered salt solution, bubbled with 95% O2-5% CO2, and maintained at 37°C. These studies were performed in the presence of indomethacin (10-5 M) to inhibit prostaglandin synthesis. In rings without endothelium, potassium chloride (10-2 to 8.5 x 10-2 M) and histamine (10-9 to 10-5 M) caused concentration-dependent contractions. When normalized to maximal contractions achieved to each agonist, the concentration-effect curves to potassium chloride and histamine in rings without endothelium were similar at each age. Rings with endothelium showed a progressive shift to the right of the concentration-effect curve to histamine, possibly secondary to an increase in the basal release of, or responsiveness to, the endothelium-derived relaxing factor with maturation. Relaxations to sodium nitroprusside (10-9 to 10-5 M) were unaffected by age. In precontracted rings, acetylcholine (10-9 to 10-6 M), bradykinin (10-10 to 10-6 M), and the calcium ionophore A23187 (10-9 to 10-6 M) caused relaxations in rings with endothelium, but not in those without endothelium, which were greater at 10 and 30 d compared to 3 d; further augmentation at 30 d compared to 10 d was not observed. In rings without endothelium, changes in the responsiveness to nitric oxide (10-9 to 10-5 M), one of the proposed endothelium-derived relaxing factors, with age were comparable to those observed with endothelium-dependent relaxing agents. These studies demonstrate that endothelium-dependent relaxations increase with age, possibly due to changes in sensitivity of the smooth muscle to the endothelium-derived relaxing factor. | en_US |
dc.language | eng | en_US |
dc.publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/ | en_US |
dc.relation.ispartof | Pediatric Research | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Animals, Newborn | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Histamine - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Drug Effects | en_US |
dc.subject.mesh | Nitric Oxide - Physiology | en_US |
dc.subject.mesh | Nitroprusside - Pharmacology | en_US |
dc.subject.mesh | Potassium Chloride - Pharmacology | en_US |
dc.subject.mesh | Pulmonary Artery - Growth & Development - Ultrastructure | en_US |
dc.subject.mesh | Swine | en_US |
dc.subject.mesh | Vasoconstriction - Drug Effects | en_US |
dc.title | Endothelium-dependent relaxations of piglet pulmonary arteries augment with maturation | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1203/00006450-199108000-00011 | - |
dc.identifier.pmid | 1896263 | - |
dc.identifier.scopus | eid_2-s2.0-0026350137 | en_US |
dc.identifier.volume | 30 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 176 | en_US |
dc.identifier.epage | 180 | en_US |
dc.identifier.isi | WOS:A1991FY61100010 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Zellers, TM=6701423788 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0031-3998 | - |