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- Publisher Website: 10.1113/jphysiol.1989.sp017475
- Scopus: eid_2-s2.0-0024495576
- PMID: 2778738
- WOS: WOS:A1989R860200034
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Article: Pertussis toxin inhibits endothelium-dependent relaxations to certain agonists in porcine coronary arteries
Title | Pertussis toxin inhibits endothelium-dependent relaxations to certain agonists in porcine coronary arteries |
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Authors | |
Issue Date | 1989 |
Publisher | Wiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751 |
Citation | Journal Of Physiology, 1989, v. 408, p. 549-560 How to Cite? |
Abstract | Pertussis toxin inactivates G(i)-protein, which mediates the inhibitory effects of receptors on adenylate cyclase. The effects of the toxin on endothelium-dependent and independent relaxations were determined in porcine coronary arteries. Arterial rings (with and without endothelium) were suspended for isometric tension recording in organ chambers filled with modified Krebs-Ringer bicarbonate solution (maintained at 37°C, gassed with 95% O2 and 5% CO2). Incubation of the tissues with pertussis toxin (100 ng/ml for 60 min) virtually abolished the endothelium-dependent relaxations produced by the α2-adrenergic agonist, UK 14304, and by 5-hydroxytryptamine. Endothelium-dependent relaxations to thrombin and to aggregating platelets were markedly reduced, whereas those produced by bradykinin were only minimally affected. Endothelium-dependent responses produced by the calcium ionophore (A23187) and by adenosine diphosphate were not altered by pertussis toxin. Pertussis toxin did not affect the direct, endothelium-independent relaxations produced by nitric oxide, or by adenosine diphosphate. These experiments demonstrate that pertussis toxin interferes with the release of endothelium-derived relaxing factor(s) evoked by certain, but not all, endothelial activators. The release of endothelium-derived relaxing factor(s) may occur through different pathways involving G(i)-protein-dependent and independent mechanisms. |
Persistent Identifier | http://hdl.handle.net/10722/170948 |
ISSN | 2023 Impact Factor: 4.7 2023 SCImago Journal Rankings: 1.708 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Flavahan, NA | en_US |
dc.contributor.author | Shimokawa, H | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:11:34Z | - |
dc.date.available | 2012-10-30T06:11:34Z | - |
dc.date.issued | 1989 | en_US |
dc.identifier.citation | Journal Of Physiology, 1989, v. 408, p. 549-560 | en_US |
dc.identifier.issn | 0022-3751 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/170948 | - |
dc.description.abstract | Pertussis toxin inactivates G(i)-protein, which mediates the inhibitory effects of receptors on adenylate cyclase. The effects of the toxin on endothelium-dependent and independent relaxations were determined in porcine coronary arteries. Arterial rings (with and without endothelium) were suspended for isometric tension recording in organ chambers filled with modified Krebs-Ringer bicarbonate solution (maintained at 37°C, gassed with 95% O2 and 5% CO2). Incubation of the tissues with pertussis toxin (100 ng/ml for 60 min) virtually abolished the endothelium-dependent relaxations produced by the α2-adrenergic agonist, UK 14304, and by 5-hydroxytryptamine. Endothelium-dependent relaxations to thrombin and to aggregating platelets were markedly reduced, whereas those produced by bradykinin were only minimally affected. Endothelium-dependent responses produced by the calcium ionophore (A23187) and by adenosine diphosphate were not altered by pertussis toxin. Pertussis toxin did not affect the direct, endothelium-independent relaxations produced by nitric oxide, or by adenosine diphosphate. These experiments demonstrate that pertussis toxin interferes with the release of endothelium-derived relaxing factor(s) evoked by certain, but not all, endothelial activators. The release of endothelium-derived relaxing factor(s) may occur through different pathways involving G(i)-protein-dependent and independent mechanisms. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751 | en_US |
dc.relation.ispartof | Journal of Physiology | en_US |
dc.subject.mesh | Adenylate Cyclase Toxin | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Coronary Vessels | en_US |
dc.subject.mesh | Endothelium, Vascular - Physiology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Methylene Blue - Pharmacology | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects | en_US |
dc.subject.mesh | Muscle Relaxation - Drug Effects | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Drug Effects | en_US |
dc.subject.mesh | Oxyhemoglobins | en_US |
dc.subject.mesh | Pertussis Toxin | en_US |
dc.subject.mesh | Platelet Aggregation | en_US |
dc.subject.mesh | Quinoxalines - Pharmacology | en_US |
dc.subject.mesh | Serotonin - Pharmacology | en_US |
dc.subject.mesh | Swine | en_US |
dc.subject.mesh | Thrombin | en_US |
dc.subject.mesh | Virulence Factors, Bordetella - Pharmacology | en_US |
dc.title | Pertussis toxin inhibits endothelium-dependent relaxations to certain agonists in porcine coronary arteries | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1113/jphysiol.1989.sp017475 | - |
dc.identifier.pmid | 2778738 | - |
dc.identifier.scopus | eid_2-s2.0-0024495576 | en_US |
dc.identifier.volume | 408 | en_US |
dc.identifier.spage | 549 | en_US |
dc.identifier.epage | 560 | en_US |
dc.identifier.isi | WOS:A1989R860200034 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Flavahan, NA=7006398882 | en_US |
dc.identifier.scopusauthorid | Shimokawa, H=16684837100 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0022-3751 | - |