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- Publisher Website: 10.1016/0003-2697(84)90085-X
- Scopus: eid_2-s2.0-0021142301
- PMID: 6731813
- WOS: WOS:A1984SK77400002
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Article: Alterations of lysophosphatidylcholine levels of canine heart: Modes of extraction and storage
Title | Alterations of lysophosphatidylcholine levels of canine heart: Modes of extraction and storage |
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Authors | |
Keywords | canine heart determination extraction lysophosphatidylcholine phospholipids |
Issue Date | 1984 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio |
Citation | Analytical Biochemistry, 1984, v. 137 n. 2, p. 277-281 How to Cite? |
Abstract | The extraction of lysophosphatidylcholine from canine heart and the storage of lysophosphatidylcholine in total lipid extracts were investigated. The lysolipid was effectively extracted from canine heart by chloroform/methanol (1/2) and the maximum recovery of the lysolipid was 89-92%. Changes in levels of the lysolipid were observed when the tissue was stored at 0°C for 60 min or at -20°C for 7 days. An alteration in the lysophosphatidylcholine level was also observed when the lipid extract from the heart was stored in theoretical lower phase (chloroform/methanol/water; 86/14/1). In order to accurately assess the level of lysophosphatidylcholine in the canine heart, the lipid should be examined immediately, from fresh tissue and the lipid extract stored in chloroform/methanol (2/1) or without solvent under nitrogen prior to separation and determination. |
Persistent Identifier | http://hdl.handle.net/10722/170722 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.493 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Mock, T | en_US |
dc.contributor.author | Pelletier, MPJ | en_US |
dc.contributor.author | Man, RYK | en_US |
dc.contributor.author | Choy, PC | en_US |
dc.date.accessioned | 2012-10-30T06:10:35Z | - |
dc.date.available | 2012-10-30T06:10:35Z | - |
dc.date.issued | 1984 | en_US |
dc.identifier.citation | Analytical Biochemistry, 1984, v. 137 n. 2, p. 277-281 | en_US |
dc.identifier.issn | 0003-2697 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/170722 | - |
dc.description.abstract | The extraction of lysophosphatidylcholine from canine heart and the storage of lysophosphatidylcholine in total lipid extracts were investigated. The lysolipid was effectively extracted from canine heart by chloroform/methanol (1/2) and the maximum recovery of the lysolipid was 89-92%. Changes in levels of the lysolipid were observed when the tissue was stored at 0°C for 60 min or at -20°C for 7 days. An alteration in the lysophosphatidylcholine level was also observed when the lipid extract from the heart was stored in theoretical lower phase (chloroform/methanol/water; 86/14/1). In order to accurately assess the level of lysophosphatidylcholine in the canine heart, the lipid should be examined immediately, from fresh tissue and the lipid extract stored in chloroform/methanol (2/1) or without solvent under nitrogen prior to separation and determination. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio | en_US |
dc.relation.ispartof | Analytical Biochemistry | en_US |
dc.subject | canine heart | - |
dc.subject | determination | - |
dc.subject | extraction | - |
dc.subject | lysophosphatidylcholine | - |
dc.subject | phospholipids | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Lysophosphatidylcholines - Isolation & Purification | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Myocardium - Analysis | en_US |
dc.subject.mesh | Solvents | en_US |
dc.subject.mesh | Specimen Handling | en_US |
dc.subject.mesh | Time Factors | en_US |
dc.title | Alterations of lysophosphatidylcholine levels of canine heart: Modes of extraction and storage | en_US |
dc.type | Article | en_US |
dc.identifier.email | Man, RYK:rykman@hkucc.hku.hk | en_US |
dc.identifier.authority | Man, RYK=rp00236 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0003-2697(84)90085-X | - |
dc.identifier.pmid | 6731813 | - |
dc.identifier.scopus | eid_2-s2.0-0021142301 | en_US |
dc.identifier.volume | 137 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 277 | en_US |
dc.identifier.epage | 281 | en_US |
dc.identifier.isi | WOS:A1984SK77400002 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Mock, T=35954418100 | en_US |
dc.identifier.scopusauthorid | Pelletier, MPJ=7102314760 | en_US |
dc.identifier.scopusauthorid | Man, RYK=7004986435 | en_US |
dc.identifier.scopusauthorid | Choy, PC=7006633002 | en_US |
dc.identifier.issnl | 0003-2697 | - |