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- Publisher Website: 10.1093/biolreprod/63.3.775
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- PMID: 10952920
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Article: Androgen control of cyclooxygenase expression in the rat epididymis
Title | Androgen control of cyclooxygenase expression in the rat epididymis |
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Authors | |
Keywords | Epididymis Signal transduction Testosterone |
Issue Date | 2000 |
Publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ |
Citation | Biology Of Reproduction, 2000, v. 63 n. 3, p. 775-780 How to Cite? |
Abstract | Bradykinin and a number of peptide hormones such as angiotensin, endothelin, and vasopressin stimulate anion secretion in rat epididymis via local formation of PGE 2. These effects are mediated by cyclooxygenase (COX)-1 isozyme. The present study was undertaken to assess the androgen control of COX expression in the epididymis. Adult male Sprague-Dawley rats were bilaterally castrated through a scrotal route. Reverse transcription-polymerase chain reaction was used to measure COX-1 and COX-2 mRNAs in the epididymis in normal and castrated rats. Anion secretion in epithelia grown from the epididymides of these rats was studied by the short-circuit current technique. In normal rats, COX-1 and COX-2 mRNAs were detected in the intact epididymis. Elimination of spermatozoa by the technique of efferent duct ligation or flushing out spermatozoa did not affect the expression of either enzyme in the epididymis, indicating that the epithelium, but not spermatozoa, expressed the enzymes. Castration caused a time-dependent decrease in expression of COX-1 and COX-2 mRNAs, which were partially restored upon testosterone replacement. In epithelia cultured from castrated rats, there was a complete loss of bradykinin-induced anion secretion. This effect was reversible upon testosterone replacement. Although epithelia from castrated rats did not respond to bradykinin, they could respond to cAMP, forskolin, and PGE 2 with only 20% loss of response magnitude when compared with epithelia from normal rats. These results suggest that the expression of COX-1 and COX-2 are dependent on androgen. The loss of COX-1 expression after castration correlates with the specific loss of anion secretion induced by bradykinin and possibly other hormones. |
Persistent Identifier | http://hdl.handle.net/10722/169742 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.022 |
ISI Accession Number ID | |
References | |
Errata |
DC Field | Value | Language |
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dc.contributor.author | Cheuk, BLY | en_US |
dc.contributor.author | Leung, PS | en_US |
dc.contributor.author | Lo, ACT | en_US |
dc.contributor.author | Wong, PYD | en_US |
dc.date.accessioned | 2012-10-25T04:54:47Z | - |
dc.date.available | 2012-10-25T04:54:47Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | Biology Of Reproduction, 2000, v. 63 n. 3, p. 775-780 | en_US |
dc.identifier.issn | 0006-3363 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/169742 | - |
dc.description.abstract | Bradykinin and a number of peptide hormones such as angiotensin, endothelin, and vasopressin stimulate anion secretion in rat epididymis via local formation of PGE 2. These effects are mediated by cyclooxygenase (COX)-1 isozyme. The present study was undertaken to assess the androgen control of COX expression in the epididymis. Adult male Sprague-Dawley rats were bilaterally castrated through a scrotal route. Reverse transcription-polymerase chain reaction was used to measure COX-1 and COX-2 mRNAs in the epididymis in normal and castrated rats. Anion secretion in epithelia grown from the epididymides of these rats was studied by the short-circuit current technique. In normal rats, COX-1 and COX-2 mRNAs were detected in the intact epididymis. Elimination of spermatozoa by the technique of efferent duct ligation or flushing out spermatozoa did not affect the expression of either enzyme in the epididymis, indicating that the epithelium, but not spermatozoa, expressed the enzymes. Castration caused a time-dependent decrease in expression of COX-1 and COX-2 mRNAs, which were partially restored upon testosterone replacement. In epithelia cultured from castrated rats, there was a complete loss of bradykinin-induced anion secretion. This effect was reversible upon testosterone replacement. Although epithelia from castrated rats did not respond to bradykinin, they could respond to cAMP, forskolin, and PGE 2 with only 20% loss of response magnitude when compared with epithelia from normal rats. These results suggest that the expression of COX-1 and COX-2 are dependent on androgen. The loss of COX-1 expression after castration correlates with the specific loss of anion secretion induced by bradykinin and possibly other hormones. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ | en_US |
dc.relation.ispartof | Biology of Reproduction | en_US |
dc.subject | Epididymis | - |
dc.subject | Signal transduction | - |
dc.subject | Testosterone | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Bradykinin - Pharmacology | en_US |
dc.subject.mesh | Cyclic Amp - Pharmacology | en_US |
dc.subject.mesh | Cyclooxygenase 1 | en_US |
dc.subject.mesh | Cyclooxygenase 2 | en_US |
dc.subject.mesh | Dinoprostone - Pharmacology | en_US |
dc.subject.mesh | Epididymis - Enzymology | en_US |
dc.subject.mesh | Epithelium - Enzymology | en_US |
dc.subject.mesh | Forskolin - Pharmacology | en_US |
dc.subject.mesh | Gene Expression Regulation, Enzymologic - Drug Effects | en_US |
dc.subject.mesh | Isoenzymes - Genetics | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Proteins | en_US |
dc.subject.mesh | Orchiectomy | en_US |
dc.subject.mesh | Prostaglandin-Endoperoxide Synthases - Genetics | en_US |
dc.subject.mesh | Rna, Messenger - Analysis | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Testosterone - Pharmacology | en_US |
dc.title | Androgen control of cyclooxygenase expression in the rat epididymis | en_US |
dc.type | Article | en_US |
dc.identifier.email | Cheuk, BLY: bernice@hku.hk | en_US |
dc.identifier.authority | Cheuk, BLY=rp01671 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1093/biolreprod/63.3.775 | - |
dc.identifier.pmid | 10952920 | - |
dc.identifier.scopus | eid_2-s2.0-0033842569 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033842569&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 63 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 775 | en_US |
dc.identifier.epage | 780 | en_US |
dc.identifier.isi | WOS:000088972400016 | - |
dc.publisher.place | United States | en_US |
dc.relation.erratum | eid:eid_2-s2.0-0033761617 | - |
dc.identifier.scopusauthorid | Cheuk, BLY=7801343617 | en_US |
dc.identifier.scopusauthorid | Leung, PS=7401748938 | en_US |
dc.identifier.scopusauthorid | Lo, ACT=36800888200 | en_US |
dc.identifier.scopusauthorid | Wong, PYD=7403980262 | en_US |
dc.identifier.issnl | 0006-3363 | - |