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- Publisher Website: 10.1007/s00418-011-0876-1
- Scopus: eid_2-s2.0-84857631069
- PMID: 22072420
- WOS: WOS:000299380200006
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Article: MiR-200b is involved in Tgf-β signaling to regulate mammalian palate development
Title | MiR-200b is involved in Tgf-β signaling to regulate mammalian palate development |
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Authors | |
Keywords | Apoptosis Cell proliferation MiR-200b Palatogenesis Smad2 Snail |
Issue Date | 2012 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm |
Citation | Histochemistry And Cell Biology, 2012, v. 137 n. 1, p. 67-78 How to Cite? |
Abstract | Various cellular and molecular events are involved in palatogenesis, including apoptosis, epithelial- mesenchymal transition (EMT), cell proliferation, and cell migration. Smad2 and Snail, which are well-known key mediators of the transforming growth factor beta (Tgf-β) pathway, play a crucial role in the regulation of palate development. Regulatory effects of microRNA 200b (miR- 200b) on Smad2 and Snail in palatogenesis have not yet been elucidated. The aim of this study is to determine the relationship between palate development regulators miR-200b and Tgf-β-mediated genes. Expression of miR-200b, E-cadherin, Smad2, and Snail was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium (MEE) and palatal mesenchyme. After the contact of palatal shelves, miR- 200b was no longer expressed in the mesenchyme around the fusion region. The binding activity of miR-200b to both Smad2 and Snail was examined using a luciferase assay. MiR-200b directly targeted Smad2 and Snail at both cellular and molecular levels. The function of miR-200b was determined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of these Tgf-β-mediated regulators and changes of apoptosis and cell proliferation in the palatal fusion region. These results suggest that miR-200b plays a crucial role in regulating the Smad2, Snail, and in apoptosis during palatogenesis by acting as a direct non-coding, influencing factor. Furthermore, the molecular interactions between miR-200b and Tgf-β signaling are important for proper palatogenesis and especially for palate fusion. Elucidating the mechanism of palatogenesis may aid the design of effective gene-based therapies for the treatment of congenital cleft palate. © Springer-Verlag 2011. |
Persistent Identifier | http://hdl.handle.net/10722/169589 |
ISSN | 2023 Impact Factor: 2.1 2023 SCImago Journal Rankings: 0.712 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, JO | en_US |
dc.contributor.author | Lee, JM | en_US |
dc.contributor.author | Cho, KW | en_US |
dc.contributor.author | Kwak, S | en_US |
dc.contributor.author | Kwon, HJ | en_US |
dc.contributor.author | Lee, MJ | en_US |
dc.contributor.author | Cho, SW | en_US |
dc.contributor.author | Kim, KS | en_US |
dc.contributor.author | Jung, HS | en_US |
dc.date.accessioned | 2012-10-25T04:53:13Z | - |
dc.date.available | 2012-10-25T04:53:13Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Histochemistry And Cell Biology, 2012, v. 137 n. 1, p. 67-78 | en_US |
dc.identifier.issn | 0948-6143 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/169589 | - |
dc.description.abstract | Various cellular and molecular events are involved in palatogenesis, including apoptosis, epithelial- mesenchymal transition (EMT), cell proliferation, and cell migration. Smad2 and Snail, which are well-known key mediators of the transforming growth factor beta (Tgf-β) pathway, play a crucial role in the regulation of palate development. Regulatory effects of microRNA 200b (miR- 200b) on Smad2 and Snail in palatogenesis have not yet been elucidated. The aim of this study is to determine the relationship between palate development regulators miR-200b and Tgf-β-mediated genes. Expression of miR-200b, E-cadherin, Smad2, and Snail was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium (MEE) and palatal mesenchyme. After the contact of palatal shelves, miR- 200b was no longer expressed in the mesenchyme around the fusion region. The binding activity of miR-200b to both Smad2 and Snail was examined using a luciferase assay. MiR-200b directly targeted Smad2 and Snail at both cellular and molecular levels. The function of miR-200b was determined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of these Tgf-β-mediated regulators and changes of apoptosis and cell proliferation in the palatal fusion region. These results suggest that miR-200b plays a crucial role in regulating the Smad2, Snail, and in apoptosis during palatogenesis by acting as a direct non-coding, influencing factor. Furthermore, the molecular interactions between miR-200b and Tgf-β signaling are important for proper palatogenesis and especially for palate fusion. Elucidating the mechanism of palatogenesis may aid the design of effective gene-based therapies for the treatment of congenital cleft palate. © Springer-Verlag 2011. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm | en_US |
dc.relation.ispartof | Histochemistry and Cell Biology | en_US |
dc.subject | Apoptosis | - |
dc.subject | Cell proliferation | - |
dc.subject | MiR-200b | - |
dc.subject | Palatogenesis | - |
dc.subject | Smad2 | - |
dc.subject | Snail | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Apoptosis | en_US |
dc.subject.mesh | Cadherins - Genetics - Metabolism | en_US |
dc.subject.mesh | Cell Proliferation | en_US |
dc.subject.mesh | Hek293 Cells | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunohistochemistry | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred Icr | en_US |
dc.subject.mesh | Micrornas - Genetics - Metabolism | en_US |
dc.subject.mesh | Palate - Cytology - Growth & Development - Metabolism | en_US |
dc.subject.mesh | Real-Time Polymerase Chain Reaction | en_US |
dc.subject.mesh | Signal Transduction - Genetics | en_US |
dc.subject.mesh | Smad2 Protein - Genetics - Metabolism | en_US |
dc.subject.mesh | Transcription Factors - Genetics - Metabolism | en_US |
dc.subject.mesh | Transforming Growth Factor Beta - Genetics - Metabolism | en_US |
dc.title | MiR-200b is involved in Tgf-β signaling to regulate mammalian palate development | en_US |
dc.type | Article | en_US |
dc.identifier.email | Jung, HS: hsjung@yuhs.ac | en_US |
dc.identifier.authority | Jung, HS=rp01683 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/s00418-011-0876-1 | en_US |
dc.identifier.pmid | 22072420 | - |
dc.identifier.scopus | eid_2-s2.0-84857631069 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-84857631069&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 137 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 67 | en_US |
dc.identifier.epage | 78 | en_US |
dc.identifier.isi | WOS:000299380200006 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Shin, JO=37361704500 | en_US |
dc.identifier.scopusauthorid | Lee, JM=41361401200 | en_US |
dc.identifier.scopusauthorid | Cho, KW=7403956665 | en_US |
dc.identifier.scopusauthorid | Kwak, S=7202607781 | en_US |
dc.identifier.scopusauthorid | Kwon, HJ=18836582500 | en_US |
dc.identifier.scopusauthorid | Lee, MJ=36054770900 | en_US |
dc.identifier.scopusauthorid | Cho, SW=32967447200 | en_US |
dc.identifier.scopusauthorid | Kim, KS=52263802700 | en_US |
dc.identifier.scopusauthorid | Jung, HS=7403030195 | en_US |
dc.identifier.citeulike | 10037318 | - |
dc.identifier.issnl | 0948-6143 | - |