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Article: Identification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry
Title | Identification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry |
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Authors | |
Keywords | 5-Fluorouracil Response Proteins Colorectal Cancer Mass Spectrometry Sw480 Two-Dimensional Electrophoresis |
Issue Date | 2008 |
Publisher | Demetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htm |
Citation | Oncology Reports, 2008, v. 20 n. 1, p. 89-98 How to Cite? |
Abstract | Colorectal cancer (CRC) is the second most prevalent cause of cancer-related deaths in the Western world. 5-Fluorouracil (5-FU) is a standard chemotherapeutic drug to treat CRC. However, the response rate is less than 20% and patients who have responded to 5-FU may become resistant. Therefore there is an urgent need to examine the 5-FU response proteins so that patients with no response to 5-FU can change to other treatment strategies promptly. In this study, the proteomic expression profile in a CRC cell line SW480 before and after 5-FU treatment was examined using 2-dimensional electrophoresis technology. Fourteen proteins with differential expression were identified using mass spectrometry and 7 of them were validated using immunocytochemical (ICC) staining. Protein identification indicated that cyclophilin A, cytokeratin 19 (CK19), cytokeratin 8 (CK8), ras-related nuclear protein, heat shock protein 27 (hsp27) and peroxiredoxin 6 (Prx 6) were upregulated whereas heat shock protein 60 (hsp60), cytokeratin 18 (CK18), cytokeratin 9 (CK9), carbamoylphosphate synthetase I, α-enolase, heat shock protein 70 (hsp70), nm23 and β-actin were down-regulated. Seven of the 14 proteins detected were validated by ICC staining, which showed that the expression of hsp27, Prx 6 and hsp70 correlated with that from proteomics profiling. Our results suggest that hsp27, Prx 6 and hsp70 are potential 5-FU response proteins and they may represent potential targets for further evaluation in other 5-FU-sensitive and -resistant CRC cell lines. |
Persistent Identifier | http://hdl.handle.net/10722/168320 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.864 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, CSZ | en_US |
dc.contributor.author | WaiKi Wong, V | en_US |
dc.contributor.author | MingLok Chan, C | en_US |
dc.contributor.author | BuigYue Ma, B | en_US |
dc.contributor.author | Pun Hui, E | en_US |
dc.contributor.author | ChiKeung Wong, M | en_US |
dc.contributor.author | YanYee Lam, M | en_US |
dc.contributor.author | ChiChuen, T | en_US |
dc.contributor.author | Chan, WH | en_US |
dc.contributor.author | Cheuk, W | en_US |
dc.contributor.author | TakCheung Chan, A | en_US |
dc.date.accessioned | 2012-10-08T03:17:32Z | - |
dc.date.available | 2012-10-08T03:17:32Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Oncology Reports, 2008, v. 20 n. 1, p. 89-98 | en_US |
dc.identifier.issn | 1021-335X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/168320 | - |
dc.description.abstract | Colorectal cancer (CRC) is the second most prevalent cause of cancer-related deaths in the Western world. 5-Fluorouracil (5-FU) is a standard chemotherapeutic drug to treat CRC. However, the response rate is less than 20% and patients who have responded to 5-FU may become resistant. Therefore there is an urgent need to examine the 5-FU response proteins so that patients with no response to 5-FU can change to other treatment strategies promptly. In this study, the proteomic expression profile in a CRC cell line SW480 before and after 5-FU treatment was examined using 2-dimensional electrophoresis technology. Fourteen proteins with differential expression were identified using mass spectrometry and 7 of them were validated using immunocytochemical (ICC) staining. Protein identification indicated that cyclophilin A, cytokeratin 19 (CK19), cytokeratin 8 (CK8), ras-related nuclear protein, heat shock protein 27 (hsp27) and peroxiredoxin 6 (Prx 6) were upregulated whereas heat shock protein 60 (hsp60), cytokeratin 18 (CK18), cytokeratin 9 (CK9), carbamoylphosphate synthetase I, α-enolase, heat shock protein 70 (hsp70), nm23 and β-actin were down-regulated. Seven of the 14 proteins detected were validated by ICC staining, which showed that the expression of hsp27, Prx 6 and hsp70 correlated with that from proteomics profiling. Our results suggest that hsp27, Prx 6 and hsp70 are potential 5-FU response proteins and they may represent potential targets for further evaluation in other 5-FU-sensitive and -resistant CRC cell lines. | en_US |
dc.language | eng | en_US |
dc.publisher | Demetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htm | en_US |
dc.relation.ispartof | Oncology Reports | en_US |
dc.subject | 5-Fluorouracil Response Proteins | en_US |
dc.subject | Colorectal Cancer | en_US |
dc.subject | Mass Spectrometry | en_US |
dc.subject | Sw480 | en_US |
dc.subject | Two-Dimensional Electrophoresis | en_US |
dc.title | Identification of 5-fluorouracil response proteins in colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chan, WH:waichan@hku.hk | en_US |
dc.identifier.authority | Chan, WH=rp00667 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-49849090629 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-49849090629&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 20 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 89 | en_US |
dc.identifier.epage | 98 | en_US |
dc.publisher.place | Greece | en_US |
dc.identifier.scopusauthorid | Wong, CSZ=26039647800 | en_US |
dc.identifier.scopusauthorid | WaiKi Wong, V=25626846000 | en_US |
dc.identifier.scopusauthorid | MingLok Chan, C=25626783000 | en_US |
dc.identifier.scopusauthorid | BuigYue Ma, B=7403301016 | en_US |
dc.identifier.scopusauthorid | Pun Hui, E=7005081895 | en_US |
dc.identifier.scopusauthorid | ChiKeung Wong, M=25626292900 | en_US |
dc.identifier.scopusauthorid | YanYee Lam, M=25626915200 | en_US |
dc.identifier.scopusauthorid | ChiChuen, T=25626079400 | en_US |
dc.identifier.scopusauthorid | Chan, WH=13310083000 | en_US |
dc.identifier.scopusauthorid | Cheuk, W=7003958432 | en_US |
dc.identifier.scopusauthorid | TakCheung Chan, A=25626859700 | en_US |
dc.identifier.issnl | 1021-335X | - |