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- Publisher Website: 10.1016/j.bios.2006.08.006
- Scopus: eid_2-s2.0-33749236579
- PMID: 16971109
- WOS: WOS:000241547500008
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Article: Flow analysis coupled with PQC/DNA biosensor for assay of E. coli based on detecting DNA products from PCR amplification
Title | Flow analysis coupled with PQC/DNA biosensor for assay of E. coli based on detecting DNA products from PCR amplification |
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Authors | |
Keywords | Biosensor E. coli Flow analysis PCR DNA sensor Piezoelectric quartz crystal |
Issue Date | 2006 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bios |
Citation | Biosensors And Bioelectronics, 2006, v. 22 n. 4 SPEC. ISS., p. 506-512 How to Cite? |
Abstract | A flow-through PQC/DNA biosensor system is developed by combining sequential flow polymerase chain reaction (PCR) products denaturing prior to piezoelectric quartz crystal (PQC) detection via hybridization of ssDNA. The PQC/DNA biosensor is fabricated based on complex formation of neutravidin/biotinylated probe in 0.2 M NaCl in TE buffer (10 mM Tris, 1 mM EDTA, pH 7.5). Results show that the coating fabricated provides a desirable quality with satisfactory performance. Its application for Escherichia coli detection under controlled flow at 0.02 mL/min for denaturing PCR products and 10 mL/min for transferring solution between reactors and delivering samples to detector to reduce rehybridization leads to significant improvement in repeatability (R.S.D. < 6%, n = 5) and sensitivity (ΔF = 34 Hz/1000 E. coli cells) as compared to existing manual method (R.S.D. = 19%, n = 5 and ΔF = 26 Hz/1000 E. coli cells, respectively). Down to 23 E. coli cells are detected, satisfying the HKEPD requirements for E. coli count in beach water. © 2006 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/168057 |
ISSN | 2023 Impact Factor: 10.7 2023 SCImago Journal Rankings: 2.052 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Sun, H | en_US |
dc.contributor.author | Zhang, Y | en_US |
dc.contributor.author | Fung, Y | en_US |
dc.date.accessioned | 2012-10-08T03:14:37Z | - |
dc.date.available | 2012-10-08T03:14:37Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Biosensors And Bioelectronics, 2006, v. 22 n. 4 SPEC. ISS., p. 506-512 | en_US |
dc.identifier.issn | 0956-5663 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/168057 | - |
dc.description.abstract | A flow-through PQC/DNA biosensor system is developed by combining sequential flow polymerase chain reaction (PCR) products denaturing prior to piezoelectric quartz crystal (PQC) detection via hybridization of ssDNA. The PQC/DNA biosensor is fabricated based on complex formation of neutravidin/biotinylated probe in 0.2 M NaCl in TE buffer (10 mM Tris, 1 mM EDTA, pH 7.5). Results show that the coating fabricated provides a desirable quality with satisfactory performance. Its application for Escherichia coli detection under controlled flow at 0.02 mL/min for denaturing PCR products and 10 mL/min for transferring solution between reactors and delivering samples to detector to reduce rehybridization leads to significant improvement in repeatability (R.S.D. < 6%, n = 5) and sensitivity (ΔF = 34 Hz/1000 E. coli cells) as compared to existing manual method (R.S.D. = 19%, n = 5 and ΔF = 26 Hz/1000 E. coli cells, respectively). Down to 23 E. coli cells are detected, satisfying the HKEPD requirements for E. coli count in beach water. © 2006 Elsevier B.V. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bios | en_US |
dc.relation.ispartof | Biosensors and Bioelectronics | en_US |
dc.subject | Biosensor | - |
dc.subject | E. coli | - |
dc.subject | Flow analysis | - |
dc.subject | PCR DNA sensor | - |
dc.subject | Piezoelectric quartz crystal | - |
dc.subject.mesh | Biosensing Techniques - Instrumentation - Methods | en_US |
dc.subject.mesh | Colony Count, Microbial - Instrumentation - Methods | en_US |
dc.subject.mesh | Dna, Bacterial - Analysis | en_US |
dc.subject.mesh | Electrochemistry - Instrumentation - Methods | en_US |
dc.subject.mesh | Environmental Monitoring - Instrumentation | en_US |
dc.subject.mesh | Equipment Design | en_US |
dc.subject.mesh | Equipment Failure Analysis | en_US |
dc.subject.mesh | Escherichia Coli - Genetics - Isolation & Purification | en_US |
dc.subject.mesh | Flow Injection Analysis - Instrumentation - Methods | en_US |
dc.subject.mesh | Polymerase Chain Reaction - Instrumentation | en_US |
dc.subject.mesh | Reproducibility Of Results | en_US |
dc.subject.mesh | Sensitivity And Specificity | en_US |
dc.subject.mesh | Water Microbiology | en_US |
dc.subject.mesh | Water Pollution - Analysis | en_US |
dc.title | Flow analysis coupled with PQC/DNA biosensor for assay of E. coli based on detecting DNA products from PCR amplification | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sun, H:sunhui@hku.hk | en_US |
dc.identifier.email | Fung, Y:ysfung@hku.hk | en_US |
dc.identifier.authority | Sun, H=rp00778 | en_US |
dc.identifier.authority | Fung, Y=rp00697 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.bios.2006.08.006 | en_US |
dc.identifier.pmid | 16971109 | - |
dc.identifier.scopus | eid_2-s2.0-33749236579 | en_US |
dc.identifier.hkuros | 134663 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33749236579&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 22 | en_US |
dc.identifier.issue | 4 SPEC. ISS. | en_US |
dc.identifier.spage | 506 | en_US |
dc.identifier.epage | 512 | en_US |
dc.identifier.eissn | 1873-4235 | - |
dc.identifier.isi | WOS:000241547500008 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Sun, H=37032038600 | en_US |
dc.identifier.scopusauthorid | Zhang, Y=49762465000 | en_US |
dc.identifier.scopusauthorid | Fung, Y=13309754700 | en_US |
dc.identifier.issnl | 0956-5663 | - |