Capillary zone electrophoresis investigation of the interaction between heparin and granulocyte-colony stimulating factor

Abstract

The interaction between standard heparin, low-molecular-weight heparin (LMWH), and granulocyte-colony stimulating factor (G-CSF) was studied by capillary zone electrophoresis. Both qualitative and quantitative characterizations of the heparin-protein binding were determined. The binding constants of the two different groups of heparins with G-CSF, calculated from the Scatchard plot by regression, were 4.805 × 105 M-1 and 4.579 × 105 M-1, respectively. The two binding constants measured are of the same order of magnitude at 105 M-1, indicating that LMWH contains most of the functional groups bound to G-CSF by standard heparin. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.