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- Publisher Website: 10.1016/0006-291X(91)91355-G
- Scopus: eid_2-s2.0-0026055970
- PMID: 1883352
- WOS: WOS:A1991GD09200031
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Article: Effect of antiserum to a 99 kDa polypeptide on the uptake of taurocholic acid by rat ileal brush border membrane vesicles
Title | Effect of antiserum to a 99 kDa polypeptide on the uptake of taurocholic acid by rat ileal brush border membrane vesicles |
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Authors | |
Issue Date | 1991 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical And Biophysical Research Communications, 1991, v. 179 n. 1, p. 204-209 How to Cite? |
Abstract | A 99 kDa polypeptide in rat ileal brush border membrane (BBM), regarded as a component of the active bile acid transport system on account of photoaffinity labeling, has been purified by affinity chromatography and preparative gel electrophoresis and utilized as an immunogen for raising polyclonal antibody. Immune serum, but not preimmune serum, specifically recognized a single band of 99 kDa protein on immunoblots of ileal and renal BBM. In contrast, no reactivity was observed with proteins in jejunal BBM. This polyclonal antibody, compared with preimmune serum and anticytosolic bile acid binding protein (14 kDa) serum, significantly inhibited the Na + dependent uptake of [ 3H] taurocholate by BBM vesicles (p < 0.01). [ 14C]D-glucose uptake by BBM vesicles was not influenced by the immune serum (p < 0.01). Thus, these studies provide further support for the specific role of a 99 kDa protein in ileal BBM bile acid transport. |
Persistent Identifier | http://hdl.handle.net/10722/167500 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Gong, YZ | en_US |
dc.contributor.author | Zwarych Jr, PP | en_US |
dc.contributor.author | Lin, MC | en_US |
dc.contributor.author | Wilson, FA | en_US |
dc.date.accessioned | 2012-10-08T03:07:46Z | - |
dc.date.available | 2012-10-08T03:07:46Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 1991, v. 179 n. 1, p. 204-209 | en_US |
dc.identifier.issn | 0006-291X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167500 | - |
dc.description.abstract | A 99 kDa polypeptide in rat ileal brush border membrane (BBM), regarded as a component of the active bile acid transport system on account of photoaffinity labeling, has been purified by affinity chromatography and preparative gel electrophoresis and utilized as an immunogen for raising polyclonal antibody. Immune serum, but not preimmune serum, specifically recognized a single band of 99 kDa protein on immunoblots of ileal and renal BBM. In contrast, no reactivity was observed with proteins in jejunal BBM. This polyclonal antibody, compared with preimmune serum and anticytosolic bile acid binding protein (14 kDa) serum, significantly inhibited the Na + dependent uptake of [ 3H] taurocholate by BBM vesicles (p < 0.01). [ 14C]D-glucose uptake by BBM vesicles was not influenced by the immune serum (p < 0.01). Thus, these studies provide further support for the specific role of a 99 kDa protein in ileal BBM bile acid transport. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_US |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies | en_US |
dc.subject.mesh | Biological Transport | en_US |
dc.subject.mesh | Electrophoresis, Polyacrylamide Gel | en_US |
dc.subject.mesh | Glucose - Metabolism | en_US |
dc.subject.mesh | Ileum - Metabolism | en_US |
dc.subject.mesh | Immunoblotting | en_US |
dc.subject.mesh | Jejunum - Metabolism | en_US |
dc.subject.mesh | Kidney - Metabolism | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Proteins - Immunology - Isolation & Purification - Metabolism | en_US |
dc.subject.mesh | Microvilli - Metabolism | en_US |
dc.subject.mesh | Molecular Weight | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Inbred Strains | en_US |
dc.subject.mesh | Taurocholic Acid - Metabolism | en_US |
dc.title | Effect of antiserum to a 99 kDa polypeptide on the uptake of taurocholic acid by rat ileal brush border membrane vesicles | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, MC:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, MC=rp00746 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0006-291X(91)91355-G | - |
dc.identifier.pmid | 1883352 | - |
dc.identifier.scopus | eid_2-s2.0-0026055970 | en_US |
dc.identifier.volume | 179 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 204 | en_US |
dc.identifier.epage | 209 | en_US |
dc.identifier.isi | WOS:A1991GD09200031 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Gong, YZ=7402475196 | en_US |
dc.identifier.scopusauthorid | Zwarych Jr, PP=8871203900 | en_US |
dc.identifier.scopusauthorid | Lin, MC=7404816359 | en_US |
dc.identifier.scopusauthorid | Wilson, FA=7202849433 | en_US |
dc.identifier.issnl | 0006-291X | - |