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- Publisher Website: 10.1016/0003-2697(85)90179-4
- Scopus: eid_2-s2.0-0022335230
- PMID: 2420226
- WOS: WOS:A1985AWR2800012
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Article: Measurement of dimethylglycine in biological fluids
Title | Measurement of dimethylglycine in biological fluids |
---|---|
Authors | |
Keywords | amino acids dimethylglycine analysis dimethylglycine dehydrogenase enzymatic assay HPLC plasma urine |
Issue Date | 1985 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio |
Citation | Analytical Biochemistry, 1985, v. 151 n. 2, p. 299-303 How to Cite? |
Abstract | The method of quantitating N,N-dimethylglycine involves cation-exchange high-performance liquid chromatography and detection of dimethylglycine with dimethylglycine dehydrogenase. Dimethylglycine was added to plasma and urine and samples were assayed for dimethylglycine. Plasma and urine to which no dimethylglycine was added were also assayed. Recoveries of added dimethylglycine were 99 to 104% with no endogenous dimethylglycine found in rat plasma or normal human urine. The human plasma used contained a small amount of endogenous dimethylglycine. The cation-exchange chromatography separates dimethylglycine from other compounds which can serve as substrates for dimethylglycine dehydrogenase. Repeatability of the assay is ±10%. Using this method we have identified dimethylglycine in the urine of a 1-month-old female human patient. |
Persistent Identifier | http://hdl.handle.net/10722/167467 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.493 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Porter, DH | en_US |
dc.contributor.author | Lin, M | en_US |
dc.contributor.author | Wagner, C | en_US |
dc.date.accessioned | 2012-10-08T03:07:23Z | - |
dc.date.available | 2012-10-08T03:07:23Z | - |
dc.date.issued | 1985 | en_US |
dc.identifier.citation | Analytical Biochemistry, 1985, v. 151 n. 2, p. 299-303 | en_US |
dc.identifier.issn | 0003-2697 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167467 | - |
dc.description.abstract | The method of quantitating N,N-dimethylglycine involves cation-exchange high-performance liquid chromatography and detection of dimethylglycine with dimethylglycine dehydrogenase. Dimethylglycine was added to plasma and urine and samples were assayed for dimethylglycine. Plasma and urine to which no dimethylglycine was added were also assayed. Recoveries of added dimethylglycine were 99 to 104% with no endogenous dimethylglycine found in rat plasma or normal human urine. The human plasma used contained a small amount of endogenous dimethylglycine. The cation-exchange chromatography separates dimethylglycine from other compounds which can serve as substrates for dimethylglycine dehydrogenase. Repeatability of the assay is ±10%. Using this method we have identified dimethylglycine in the urine of a 1-month-old female human patient. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio | en_US |
dc.relation.ispartof | Analytical Biochemistry | en_US |
dc.subject | amino acids | - |
dc.subject | dimethylglycine analysis | - |
dc.subject | dimethylglycine dehydrogenase | - |
dc.subject | enzymatic assay | - |
dc.subject | HPLC | - |
dc.subject | plasma | - |
dc.subject | urine | - |
dc.subject.mesh | Carbon Radioisotopes | en_US |
dc.subject.mesh | Chromatography, High Pressure Liquid - Methods | en_US |
dc.subject.mesh | Dimethylglycine Dehydrogenase | en_US |
dc.subject.mesh | Glycine - Analogs & Derivatives | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Mitochondrial Proteins | en_US |
dc.subject.mesh | Oxidoreductases, N-Demethylating - Metabolism | en_US |
dc.subject.mesh | Radioisotope Dilution Technique | en_US |
dc.subject.mesh | Sarcosine - Analogs & Derivatives - Blood - Urine | en_US |
dc.subject.mesh | Substrate Specificity | en_US |
dc.title | Measurement of dimethylglycine in biological fluids | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, M:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, M=rp00746 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0003-2697(85)90179-4 | - |
dc.identifier.pmid | 2420226 | - |
dc.identifier.scopus | eid_2-s2.0-0022335230 | en_US |
dc.identifier.volume | 151 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 299 | en_US |
dc.identifier.epage | 303 | en_US |
dc.identifier.isi | WOS:A1985AWR2800012 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Porter, DH=36847248400 | en_US |
dc.identifier.scopusauthorid | Lin, M=7404816359 | en_US |
dc.identifier.scopusauthorid | Wagner, C=7402148945 | en_US |
dc.identifier.issnl | 0003-2697 | - |