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Article: Effect of CD8+ T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infection
Title | Effect of CD8+ T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infection 體內CD8+T細胞剔除對無癥狀期SHIV感染猴的影 |
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Authors | |
Keywords | Macaca mulatta (恒河猴) HIV T-Lymphocytes Infection Viruses |
Issue Date | 2011 |
Publisher | 中國實驗動物學會 |
Citation | 中國比較醫學雜誌, 2011, v. 21 n. 4, p. 1-5 How to Cite? Chinese Journal of Comparative Medicine, 2011, v. 21 n. 4, p. 1-5 How to Cite? |
Abstract | 目的 CD8+T細胞在一些病毒感染疾病的免疫反應中起著重要的作用,但CD8+T細胞在HIV無癥狀期的作用尚不明確,本研究通過體內CD8+T細胞剔除,研究CD8+T細胞對SHIV感染猴的影響,進一步了解艾滋病的發病機制。方法選擇8只SHIV病毒感染的恒河猴,均處于無癥狀期,隨機分成兩組,實驗組4只恒河猴在0、3、7 d注射抗CD8+T抗體cM-T807,不同的時間取外周血、腹股溝淋巴結。流式細胞術測定恒河猴外周血和淋巴結中CD8+T細胞數目,Real-time RT-PCR法測定實驗猴血漿病毒載量,并使用IFN-γElispot方法測定其對猴細胞免疫的影響。結果 CD8+T細胞敲除后,4只猴的病毒載量都轉陽,但反應性不一,HIV-1的靶細胞CD4+T細胞有輕微下降,后反彈,與病毒載量無相關性;CD8敲除猴的感染情況(血漿病毒載量和CD4細胞)比SHIV病毒急性感染輕,這與ELIPOT結果一致。結論 CD8+T細胞在HIV無癥狀期發揮重要的作用,但其作用具有個體差別。 Objective CD8+ T cells play an important role in some viral diseases,but the role of CD8+ T cells is not yet fully clear in the asymptomatic phase of HIV infection.The aim of this study was to elucidate the impact of CD8 + T cell in SHIV-infected monkeys with in vivo CD8 + T cells depletion,and to further understand the pathogenesis of AIDS.Methods Eight SHIV-infected rhesus monkeys in the asymptomatic phase were selected and randomly divided into two groups.Anti-CD8 + T antibody cM-T807 were injected intravenously into four monkeys on days 0,3,and 7.Then the CD8 + T cell number in peripheral blood and lymph nodes in the monkeys were detected by flow cytometry,the plasma viral load by real-time RT-PCR,and the cellular immunity by IFN-γ Elispot assay.Results The viral load of the four monkeys was elevated to above of detected after the CD8 + T cell depletion,but the reactions were not uniform.CD4 + T cells,the HIV-1 target cells,rebound and dropped slightly.The infection of monkeys with CD8 + T cell depletion was lighter than that after the SHIV virus first infection(plasma viral load and CD4 cells),which was consistent with the Elispot results.Conclusions CD8 + T cells play an important role in the asymptomatic phase of HIV-1 infection,but with some interindividual variation. |
Persistent Identifier | http://hdl.handle.net/10722/164388 |
ISSN |
DC Field | Value | Language |
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dc.contributor.author | Wang, W | en_US |
dc.contributor.author | Cong, Z | en_US |
dc.contributor.author | Liu, H | en_US |
dc.contributor.author | Tao, Z | en_US |
dc.contributor.author | Liu, Q | en_US |
dc.contributor.author | Wei, Q | en_US |
dc.contributor.author | Chen, Z | en_US |
dc.contributor.author | Qin, C | en_US |
dc.date.accessioned | 2012-09-20T07:58:49Z | - |
dc.date.available | 2012-09-20T07:58:49Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | 中國比較醫學雜誌, 2011, v. 21 n. 4, p. 1-5 | en_US |
dc.identifier.citation | Chinese Journal of Comparative Medicine, 2011, v. 21 n. 4, p. 1-5 | - |
dc.identifier.issn | 1671-7856 | - |
dc.identifier.uri | http://hdl.handle.net/10722/164388 | - |
dc.description.abstract | 目的 CD8+T細胞在一些病毒感染疾病的免疫反應中起著重要的作用,但CD8+T細胞在HIV無癥狀期的作用尚不明確,本研究通過體內CD8+T細胞剔除,研究CD8+T細胞對SHIV感染猴的影響,進一步了解艾滋病的發病機制。方法選擇8只SHIV病毒感染的恒河猴,均處于無癥狀期,隨機分成兩組,實驗組4只恒河猴在0、3、7 d注射抗CD8+T抗體cM-T807,不同的時間取外周血、腹股溝淋巴結。流式細胞術測定恒河猴外周血和淋巴結中CD8+T細胞數目,Real-time RT-PCR法測定實驗猴血漿病毒載量,并使用IFN-γElispot方法測定其對猴細胞免疫的影響。結果 CD8+T細胞敲除后,4只猴的病毒載量都轉陽,但反應性不一,HIV-1的靶細胞CD4+T細胞有輕微下降,后反彈,與病毒載量無相關性;CD8敲除猴的感染情況(血漿病毒載量和CD4細胞)比SHIV病毒急性感染輕,這與ELIPOT結果一致。結論 CD8+T細胞在HIV無癥狀期發揮重要的作用,但其作用具有個體差別。 | - |
dc.description.abstract | Objective CD8+ T cells play an important role in some viral diseases,but the role of CD8+ T cells is not yet fully clear in the asymptomatic phase of HIV infection.The aim of this study was to elucidate the impact of CD8 + T cell in SHIV-infected monkeys with in vivo CD8 + T cells depletion,and to further understand the pathogenesis of AIDS.Methods Eight SHIV-infected rhesus monkeys in the asymptomatic phase were selected and randomly divided into two groups.Anti-CD8 + T antibody cM-T807 were injected intravenously into four monkeys on days 0,3,and 7.Then the CD8 + T cell number in peripheral blood and lymph nodes in the monkeys were detected by flow cytometry,the plasma viral load by real-time RT-PCR,and the cellular immunity by IFN-γ Elispot assay.Results The viral load of the four monkeys was elevated to above of detected after the CD8 + T cell depletion,but the reactions were not uniform.CD4 + T cells,the HIV-1 target cells,rebound and dropped slightly.The infection of monkeys with CD8 + T cell depletion was lighter than that after the SHIV virus first infection(plasma viral load and CD4 cells),which was consistent with the Elispot results.Conclusions CD8 + T cells play an important role in the asymptomatic phase of HIV-1 infection,but with some interindividual variation. | - |
dc.language | chi | en_US |
dc.publisher | 中國實驗動物學會 | - |
dc.relation.ispartof | 中國比較醫學雜誌 | en_US |
dc.relation.ispartof | Chinese Journal of Comparative Medicine | - |
dc.subject | Macaca mulatta (恒河猴) | - |
dc.subject | HIV | - |
dc.subject | T-Lymphocytes | - |
dc.subject | Infection | - |
dc.subject | Viruses | - |
dc.title | Effect of CD8+ T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infection | en_US |
dc.title | 體內CD8+T細胞剔除對無癥狀期SHIV感染猴的影 | - |
dc.type | Article | en_US |
dc.identifier.email | Chen, Z: zchenai@hku.hk | en_US |
dc.identifier.authority | Chen, Z=rp00243 | en_US |
dc.identifier.doi | 10.3969/j.issn.1671.7856.2011.04.001 | - |
dc.identifier.hkuros | 206277 | en_US |
dc.identifier.volume | 21 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 1 | en_US |
dc.identifier.epage | 5 | en_US |
dc.publisher.place | China | - |
dc.identifier.issnl | 1671-7856 | - |