Biomimic enamel remineralization by hybridization calcium-and phosphate-loaded liposomes with amelogenin-inspired peptide

Abstract

We here report a novel biomimetic mineralization strategy for enamel remineralization by intergration of calcium phosphate loaded and thermally triggered liposomes and a self-assembly amelogenin-inspired peptide. Firstly, calcium and phosphate loaded temperature sensitive liposomes were synthesized by Interdigitation-fusion method with 1,2-bis(palmitoyl)-sn-glycero-3-phosphor- choline (DPPC) and 1,2-bis(myristoyl)-sn-glycero- 3-phosphocholine (DMPC) at mass ratio of 9:1. The liposomes were stable at room temperature, but slowly released calcium and phosphate ions if heated to 37 °C. Secondly, a novel polyanion amelogenin-inspired oligopeptide (Gln-Pro-Ala)4-Thr-Lys- Arg-Glu-Glu-Val-Asp) was synthesized by standard solid-phase. Finally, the mixture of peptide and liposomes solution was exposed to enamel surface at 37 °C. The results showed oriented enamel-like hydroxylapatite evenly deposited on enamel surface. © (2012) Trans Tech Publications.