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Conference Paper: Response of the human peritoneal mesothelial cell to injury: An in vitro model of peritoneal wound healing
Title | Response of the human peritoneal mesothelial cell to injury: An in vitro model of peritoneal wound healing |
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Authors | |
Keywords | Cell behavior Dialysate End-stage renal failure Infection Peritoneal injury |
Issue Date | 1998 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/ki/index.html |
Citation | Meeting of the International Society of Nephrology, Do¨lln, Germany, 1998. In Kidney International, 1998, v. 54 n. 6, p. 2160-2169 How to Cite? |
Abstract | Background. The denudation of the peritoneal mesothelium and damage to the underlying interstitium is a frequent finding in patients receiving continuous ambulatory peritoneal dialysis as a treatment for end-stage renal failure. The response of the mesothelium to injury from repeated episodes of infection or from exposure to dialysis fluids has not been extensively studied. The present study describes a simple and reproducible method with which to investigate the response of human mesothelial cells to injury. Methods. The model of peritoneal injury consists of mechanically wounding a monolayer of human peritoneal mesothelial cells with a glass probe and following the repopulation of the denuded area by time-lapse photomicroscopy. In addition immunohistochemistry was used to follow the response of marker proteins for stress fibers and focal adhesions as well as macromolecules associated with the extracellular matrix. Results. Under serum-free conditions the wound (0.58 ± 0.094 mm; mean ± SD; N = 20) closed within 72 ± 5 hours (N = 8). This rate of healing was enhanced by fetal calf serum, by human serum (10%) and by undiluted spent non-infected dialysate. The repair process over the first 48 hours was the result of cell migration, was independent of cell proliferation and involved the de novo synthesis of several different extracellular matrix components. An early event in the healing process was the rapid reorganization of intracellular stress fibers together with the formation of associated focal adhesions in cells at the wound edge. Conclusion. This in vitro model should prove invaluable in characterizing the process of wound healing within the peritoneal cavity, thus allowing a better understanding of the response to infection as well as any effect of dialysis fluids in this pattern of cell behavior. |
Description | Conference Theme: Genes and Genetics in Hypertension and Vascular Disease Session: Basic Science – Biocompatibility |
Persistent Identifier | http://hdl.handle.net/10722/163546 |
ISSN | 2023 Impact Factor: 14.8 2023 SCImago Journal Rankings: 3.886 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yung, S | en_US |
dc.contributor.author | Davies, M | en_US |
dc.date.accessioned | 2012-09-05T05:37:18Z | - |
dc.date.available | 2012-09-05T05:37:18Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Meeting of the International Society of Nephrology, Do¨lln, Germany, 1998. In Kidney International, 1998, v. 54 n. 6, p. 2160-2169 | en_US |
dc.identifier.issn | 0085-2538 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/163546 | - |
dc.description | Conference Theme: Genes and Genetics in Hypertension and Vascular Disease | - |
dc.description | Session: Basic Science – Biocompatibility | - |
dc.description.abstract | Background. The denudation of the peritoneal mesothelium and damage to the underlying interstitium is a frequent finding in patients receiving continuous ambulatory peritoneal dialysis as a treatment for end-stage renal failure. The response of the mesothelium to injury from repeated episodes of infection or from exposure to dialysis fluids has not been extensively studied. The present study describes a simple and reproducible method with which to investigate the response of human mesothelial cells to injury. Methods. The model of peritoneal injury consists of mechanically wounding a monolayer of human peritoneal mesothelial cells with a glass probe and following the repopulation of the denuded area by time-lapse photomicroscopy. In addition immunohistochemistry was used to follow the response of marker proteins for stress fibers and focal adhesions as well as macromolecules associated with the extracellular matrix. Results. Under serum-free conditions the wound (0.58 ± 0.094 mm; mean ± SD; N = 20) closed within 72 ± 5 hours (N = 8). This rate of healing was enhanced by fetal calf serum, by human serum (10%) and by undiluted spent non-infected dialysate. The repair process over the first 48 hours was the result of cell migration, was independent of cell proliferation and involved the de novo synthesis of several different extracellular matrix components. An early event in the healing process was the rapid reorganization of intracellular stress fibers together with the formation of associated focal adhesions in cells at the wound edge. Conclusion. This in vitro model should prove invaluable in characterizing the process of wound healing within the peritoneal cavity, thus allowing a better understanding of the response to infection as well as any effect of dialysis fluids in this pattern of cell behavior. | en_US |
dc.language | eng | en_US |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/ki/index.html | en_US |
dc.relation.ispartof | Kidney International | en_US |
dc.subject | Cell behavior | - |
dc.subject | Dialysate | - |
dc.subject | End-stage renal failure | - |
dc.subject | Infection | - |
dc.subject | Peritoneal injury | - |
dc.subject.mesh | Blood Physiological Phenomena | en_US |
dc.subject.mesh | Cell Division - Physiology | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Cytoskeleton - Physiology | en_US |
dc.subject.mesh | Dialysis Solutions - Pharmacology | en_US |
dc.subject.mesh | Epithelium - Drug Effects - Pathology - Physiopathology | en_US |
dc.subject.mesh | Extracellular Matrix - Physiology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Peritoneum - Drug Effects - Injuries - Pathology - Physiopathology | en_US |
dc.subject.mesh | Time Factors | en_US |
dc.subject.mesh | Wound Healing - Drug Effects - Physiology | en_US |
dc.title | Response of the human peritoneal mesothelial cell to injury: An in vitro model of peritoneal wound healing | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Yung, S:ssyyung@hku.hk | en_US |
dc.identifier.authority | Yung, S=rp00455 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1046/j.1523-1755.1998.00177.x | en_US |
dc.identifier.pmid | 9853283 | - |
dc.identifier.scopus | eid_2-s2.0-0031786809 | en_US |
dc.identifier.hkuros | 46254 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0031786809&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 54 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 2160 | en_US |
dc.identifier.epage | 2169 | en_US |
dc.identifier.isi | WOS:000077129000039 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Yung, S=22636568800 | en_US |
dc.identifier.scopusauthorid | Davies, M=7404207291 | en_US |
dc.customcontrol.immutable | sml 170223 amended | - |
dc.identifier.issnl | 0085-2538 | - |