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- Publisher Website: 10.1016/j.pep.2011.03.013
- Scopus: eid_2-s2.0-79960133528
- PMID: 21453774
- WOS: WOS:000292996100001
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Article: High level expression, purification and characterization of active fusion human C1q and tumor necrosis factor related protein 2 (hCTRP2) in Escherichia coli
Title | High level expression, purification and characterization of active fusion human C1q and tumor necrosis factor related protein 2 (hCTRP2) in Escherichia coli |
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Authors | |
Keywords | Escherichia coli Expression and purification Fusion protein Human C1q and tumor necrosis factor related protein 2 (hCTRP2) Pichia pastoris |
Issue Date | 2011 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprep |
Citation | Protein Expression And Purification, 2011, v. 79 n. 1, p. 1-6 How to Cite? |
Abstract | C1q and tumor necrosis factor related proteins (CTRPs) are a family of adiponectin paralogues. Among them, CTRP2 is the only CTRP protein that has been shown to possess similar biological activities as adiponectin. To further explore the physiological roles of human CTRP2 and its mechanisms of action, hCTRP2 gene was expressed in Escherichia coli and Pichia pastoris, respectively. In the P. pastoris expression system, recombinant hCTRP2 could be secreted into the culture medium under induction condition, however, the resultant recombinant protein was highly unstable, resulting two main degradation products with molecular masses of approximately 20 and 26 kDa, respectively. In the E. coli expression system, a large amount of soluble thioredoxin (Trx)-hCTRP2 fusion protein could be produced, which accounts about 42% of the total soluble bacterial proteins. The recombinant Trx-hCTRP2 fusion protein was purified to an approximately 95% purity using Ni-NTA affinity chromatography and Superdex G-75 column with a yield of about 15 mg/l protein from 1 l bacterial culture. The purified recombinant Trx-hCTRP2 was shown to be active under in vitro assay conditions. © 2011 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/163385 |
ISSN | 2023 Impact Factor: 1.4 2023 SCImago Journal Rankings: 0.383 |
ISI Accession Number ID | |
References | |
Errata |
DC Field | Value | Language |
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dc.contributor.author | Li, H | en_HK |
dc.contributor.author | Gao, X | en_HK |
dc.contributor.author | Zhou, Y | en_HK |
dc.contributor.author | Li, N | en_HK |
dc.contributor.author | Ge, C | en_HK |
dc.contributor.author | Hui, X | en_HK |
dc.contributor.author | Wang, Y | en_HK |
dc.contributor.author | Xu, A | en_HK |
dc.contributor.author | Jin, S | en_HK |
dc.contributor.author | Wu, D | en_HK |
dc.date.accessioned | 2012-09-05T05:30:46Z | - |
dc.date.available | 2012-09-05T05:30:46Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Protein Expression And Purification, 2011, v. 79 n. 1, p. 1-6 | en_HK |
dc.identifier.issn | 1046-5928 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/163385 | - |
dc.description.abstract | C1q and tumor necrosis factor related proteins (CTRPs) are a family of adiponectin paralogues. Among them, CTRP2 is the only CTRP protein that has been shown to possess similar biological activities as adiponectin. To further explore the physiological roles of human CTRP2 and its mechanisms of action, hCTRP2 gene was expressed in Escherichia coli and Pichia pastoris, respectively. In the P. pastoris expression system, recombinant hCTRP2 could be secreted into the culture medium under induction condition, however, the resultant recombinant protein was highly unstable, resulting two main degradation products with molecular masses of approximately 20 and 26 kDa, respectively. In the E. coli expression system, a large amount of soluble thioredoxin (Trx)-hCTRP2 fusion protein could be produced, which accounts about 42% of the total soluble bacterial proteins. The recombinant Trx-hCTRP2 fusion protein was purified to an approximately 95% purity using Ni-NTA affinity chromatography and Superdex G-75 column with a yield of about 15 mg/l protein from 1 l bacterial culture. The purified recombinant Trx-hCTRP2 was shown to be active under in vitro assay conditions. © 2011 Elsevier Inc. All rights reserved. | en_HK |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprep | en_HK |
dc.relation.ispartof | Protein Expression and Purification | en_HK |
dc.subject | Escherichia coli | en_HK |
dc.subject | Expression and purification | en_HK |
dc.subject | Fusion protein | en_HK |
dc.subject | Human C1q and tumor necrosis factor related protein 2 (hCTRP2) | en_HK |
dc.subject | Pichia pastoris | en_HK |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Escherichia Coli - Genetics | en_US |
dc.subject.mesh | Gene Expression | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Pichia - Genetics | en_US |
dc.subject.mesh | Proteins - Genetics - Isolation & Purification - Metabolism | en_US |
dc.subject.mesh | Recombinant Fusion Proteins - Genetics - Isolation & Purification - Metabolism | en_US |
dc.title | High level expression, purification and characterization of active fusion human C1q and tumor necrosis factor related protein 2 (hCTRP2) in Escherichia coli | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Wang, Y: yuwanghk@hku.hk | en_HK |
dc.identifier.email | Xu, A: amxu@hkucc.hku.hk | en_HK |
dc.identifier.authority | Wang, Y=rp00239 | en_HK |
dc.identifier.authority | Xu, A=rp00485 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.pep.2011.03.013 | en_HK |
dc.identifier.pmid | 21453774 | - |
dc.identifier.scopus | eid_2-s2.0-79960133528 | en_HK |
dc.identifier.hkuros | 206391 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79960133528&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 79 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 1 | en_HK |
dc.identifier.epage | 6 | en_HK |
dc.identifier.isi | WOS:000292996100001 | - |
dc.publisher.place | United States | en_HK |
dc.relation.erratum | doi: 10.1016/j.pep.2011.11.005 | - |
dc.identifier.scopusauthorid | Li, H=40261980700 | en_HK |
dc.identifier.scopusauthorid | Gao, X=26028577700 | en_HK |
dc.identifier.scopusauthorid | Zhou, Y=25654258200 | en_HK |
dc.identifier.scopusauthorid | Li, N=36065390000 | en_HK |
dc.identifier.scopusauthorid | Ge, C=54389034600 | en_HK |
dc.identifier.scopusauthorid | Hui, X=26666795900 | en_HK |
dc.identifier.scopusauthorid | Wang, Y=34973733700 | en_HK |
dc.identifier.scopusauthorid | Xu, A=7202655409 | en_HK |
dc.identifier.scopusauthorid | Jin, S=7401822323 | en_HK |
dc.identifier.scopusauthorid | Wu, D=7404297751 | en_HK |
dc.identifier.citeulike | 9126065 | - |
dc.identifier.issnl | 1046-5928 | - |